Use of Activin A in human embryo stem cell trophoblast-free cell culture

A technology of human embryonic stem cells and trophoblast cells, applied in the field of biomedicine, can solve the problem that the in vitro culture conditions of human embryonic stem cells are not optimal, etc.
CN101008002AActive Publication Date: 2007-08-01浙江煦顼技术有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
浙江煦顼技术有限公司
Publication Date
2007-08-01

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Abstract

The invention discloses the application of Activin A for cell culture in human blast stem cell panoistic layer, belonging to biomedical technology field. It is necessary and adequate for Activin A to maintain self- refresh and multi-function for human blast stem cell, it can induce expression of transcription factor Oct4 and Nanog. The stem cell still possess differentiation potence in mouse body for teratoma after 10- generation culture with panoistic cell culture medium containing 5 ng / ml Activin A, and it still maintains normal karyotype after 150- day culture and more than 20- generation.
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Description

technical field

[0001] The invention belongs to the technical field of biomedicine, in particular, the invention relates to the application of transforming growth factor Activin A for culture of human embryonic stem cells without trophoblast. Background technique

[0002] Human embryonic stem cells are cells obtained from the inner cell mass of early human embryos under in vitro culture conditions and have the potential to develop into any type of cell in the body and have unlimited proliferation potential (Thomson JA., Itskovitz-Eldor J., Shapiro SS., et al. Science. Nov 6 1998;282(5391):1145-1147). Human embryonic stem cells have the ability to expand infinitely under specific culture conditions in vitro, maintain a normal karyotype and differentiate into any type of cell in the body, which provides an unlimited source of cells for the treatment of human diseases and damaged tissues. These diseases that may be treated with human embryonic stem cells include: diabetes, Alz...

Claims

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