237 results about "Mouse Testis" patented technology

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the testis size character of Hu sheep as well as a screening method and application of the SNP molecular marker, and belongs to the fields of genetic breeding and molecular biology. The marker is located in an intron region of a Hu sheep chromosome 3 LRRIQ1 gene and is remarkably related to the Hu sheep testis size character, and the accuracy and reliability of the SNP molecular marker are ensured through a scientific and rigorous screening method; according to the marker, Hu sheep individuals related to testis size characters can be rapidly and accurately screened out, the breeding efficiency and accuracy are improved, the breeding process is accelerated, the breeding cost is reduced, and the economic benefits are improved; the genetic diversity is protected, the accuracy of seminal fluid quality evaluation is improved, a powerful tool is provided for Hu sheep breeding work, and important basic data is also provided for researching a genetic mechanism of testis development and functions; the method can also be used as an auxiliary diagnosis tool to assist in identifying potential genetic defects or disease risks and promote progress and development of the breeding technology.

The invention relates to the technical field of biology, in particular to multifunctional belly-expanding hippocampal peptide as well as a preparation method and application thereof. Comprising a polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The hippocampus antioxidative peptide is prepared by adopting an ultrahigh pressure pretreatment and enzymolysis technology, the adopted extraction process is simple in route, and the time and the production cost are effectively saved; an animal model is utilized to prove the anti-oxidation effect and the effect of improving male reproductive disorder and testis injury of the multifunctional belly-expanding hippocampal peptide; and on the basis, a novel peptide fragment with high activity, no toxicity and no sensitization is obtained.

The invention relates to the technical field of biological medicines, in particular to an umbilical cord mesenchymal stem cell exosome and application of the umbilical cord mesenchymal stem cell exosome in preparation of a medicine for treating male infertility caused by hidden testis. The prepared high-concentration umbilical cord mesenchymal stem cell exosome suspension preparation is directly used for treating cultured spermatogonial cells and spermatoblasts in vitro, is injected into the testis of a mouse, and is used for improving the seminiferous tubule and spermatogenic microenvironment of testis tissue and promoting division and proliferation of the spermatogonial cells and the spermatoblasts. The method can effectively reverse oligospermia and azoospermia caused by the hidden testis, and compared with a model without intervention of treatment means, the method can effectively promote increase and even regeneration of sperms in the testis.

The invention belongs to the technical field of medicines, and particularly discloses application of niclosamide in preparation of a medicine for treating epididymis and testis fibrosis caused by bacterial infection. A UPEG-mouse epididymis / orchitis model is firstly constructed, a result shows that a monocyte subset highly expressed by S100a4 appears in a testis, the group of cells mainly express CD11b monocyte characteristics in the early stage when entering the testis, and the group of cells are partially converted into F4 / 80 + and alpha-SMA + fibroblast characteristics in the later stage. A myeloid macrophage knockout mouse is used for constructing a UPEG-epididymis / orchitis model, and the fibrosis degree of testis and epididymis is obviously reduced; a molecular mechanism shows that S100a4 can strengthen a TGF beta-STAT3 signal channel to promote the transformation of macrophages to fibroblasts, and niclosamide can inhibit the process. Mouse in-vivo experiment results show that niclosamide can obviously inhibit UPEG-mouse epididymis and testis fibrosis.

The invention provides a kit for detecting spermatogenic ability of azoospermia patients and application thereof, and belongs to the technical field of reproductive medicines.The kit comprises the following reagents: testis tissue digestive juice and testis single cell flow staining fluid; the testis single cell flow type staining solution comprises a DNA (Deoxyribose Nucleic Acid) staining solution Hoechst 33342 and an RNA (Ribonucleic Acid) staining solution # imgabs0 # RNASelectTM green fluorescent cell staining agent; according to the kit, double staining of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) is carried out on testis single cells, and a fluorescence activated cell sorting technique is matched, so that refining of testis single cell subgroups of azoospermia patients is realized, and the spermatogenic ability of the azoospermia patients is determined according to a testis single cell subgroup subdivision result. The kit disclosed by the invention is used for pre-operation evaluation of a microscopic sperm extraction operation, is used for judging whether a patient has spermatogenic ability and is suitable for microscopic sperm extraction or not, can also be used for guiding the patient to further clarify the cause of disease, and has important clinical significance.

The invention relates to the technical field of animal vaccines, and discloses an animal recombinant castration vaccine and a preparation method and application thereof, the animal recombinant castration vaccine comprises an animal castration vaccine antigen and an adjuvant, and the content of the animal castration vaccine antigen in the vaccine is 0.5-1 mg / mL. A gel type vaccine is prepared by adopting an animal castration vaccine antigen with an amino acid sequence as shown in SEQ ID No.2-4, and testis growth and development of rats are obviously inhibited after the rats are immunized. The animal castration vaccine antigen is also used for preparing vaccines in three dosage forms, namely an emulsion type vaccine, an aluminum salt type vaccine and a gel type vaccine, after the three vaccines are used for immunizing rats, the anti-GnRH antibody titer in the rats is obviously increased, the testosterone concentration is reduced (Plt; 0.05), and the testis growth and development are inhibited. The three dosage forms of vaccines all have good immune castration effect, and can be applied to castration or estrus inhibition of pets and economic animals in the future.

The invention discloses a traditional Chinese medicine compound oral preparation for treating male oligoasthenospermia, and belongs to the technical field of traditional Chinese medicine preparations. The rhizoma polygonati processed with wine, the prepared rehmannia root and the like are used for nourishing the liver and kidney to consolidate congenital, the codonopsis pilosula, the astragalus membranaceus and the like are used for invigorating spleen and replenishing qi to complement postnatal, the fried cowherb seed is used for activating blood circulation, and the salted semen plantaginis is used for promoting diuresis, so that the problems of insufficient spermatogenesis and low vitality caused by deficiency of the spleen and the kidney are fundamentally solved. Experiments and clinical researches show that the preparation can significantly improve the sperm quantity, activity and activity rate of oligoasthenospermia model mice and patients, the clinical total effective rate reaches 90%, the pregnancy rate reaches 53.3%, and the curative effect is superior to that of Wuzi Yanzong pills in a control group. The action mechanism covers regulation of blood lipid metabolism, improvement of hypothalamus-pituitary-gonad axis functions, repair of testicular seminiferous tubule structural damage and reduction of lipid deposition, and meanwhile, oxidative stress and inflammatory injury are relieved and spermatogenic cell functions are protected by regulating and controlling a PPAR signal channel and inhibiting molecular pathways such as Cga / Sting mediated cell apoptosis.

The invention relates to the technical field of reproductive biology, in particular to a method for efficiently constructing pig testis organs in vitro. Comprising the following steps: S1, enzymolysis of testicular tissues of piglets; and S2, testicular cell resuspension counting: uniformly suspending the testicular cells obtained after enzymolysis in the step S1, sequentially collecting the cells into a 96-well plate with a U-shaped bottom, and adding a DMEM base culture medium containing 5% of FBS, 10% of KSR and 1% of P / S into each well to complete resuspension culture of the cells. S3, testicular organ in-vitro culture: separating testicular cell masses, transferring the separated cell masses into a 96-well plate with a U-shaped bottom, and adding the cell masses into a DMEM base culture medium containing 5% of FBS, 10% of KSR and 1% of P / S for culture; and the quantity of cells in the 96-well plate with the U-shaped bottom is 5 * 104 cells per well. After the culture system is optimized, the experimental process is simplified, the testicular organs can be directly cultured in the 96-well plate with the U-shaped bottom, the replacement frequency is reduced, the pollution risk is reduced, the number of adopted cells is remarkably reduced, and the culture efficiency is improved.

The invention provides application of a CD47 gene in detection of pig litter size phenotype and application of an SNP site of the CD47 gene in gene expression regulation. It is found that the CD47 gene is regulated and controlled by the SNP site rs323354626, it is further found that the rs323354626 site and the phenotype of the total litter size of sows show the highest significance, and it is indicated that the CD47 gene and the total litter size of the sows have large correlation. In three main commercial varieties of pigs, namely Duroc, Landrace and Yorkshire, the CD47 expression level in testis tissues is negatively correlated with the litter size, which indicates that the expression mode of the CD47 gene may influence the reproductive performance of the pigs, and the litter size phenotype of the pigs can be detected by detecting the CD47 gene expression level in the testis of the breeding pigs or somatic cells of the sows. Or the litter size of the pigs is influenced by regulating and controlling the expression level of the CD47 gene through the rs323354626 site, and the discovery lays a foundation for improving the breeding efficiency of the pigs, especially for a breeding method for increasing the litter size.

The invention provides a method for culturing primary spermatogonial stem cells of Tibetan sheep, and belongs to the technical field of cell separation and culture. The culture method provided by the invention comprises the following steps: disinfecting and cleaning testis tissues of Tibetan sheep lambs; shearing tissue blocks, and digesting and cleaning to obtain a single-cell suspension of the seminiferous tubule; purifying the single-cell suspension, and culturing without a feeding layer or with the feeding layer; the culture without the feeding layer refers to culture in a laminin-coated culture container by using a feeding-layer-free MEM alpha culture medium or a StemProTM-34SFM complete culture medium; culture with a feeding layer refers to culture in a mouse fibroblast line feeding layer or a Tibetan sheep primary testicular sertoli feeding layer, and a feeding layer MEMS alpha culture medium is added. According to the culture method provided by the invention, the Tibetan sheep spermatogonial stem cells with higher purity can be obtained through separation, the subsequent proliferation of the spermatogonial stem cells can be remarkably promoted, and the apoptosis of the spermatogonial stem cells can be inhibited.

The invention relates to the technical field of biology, in particular to application of rosa roxburghii tratt active extract in preparation of a medicine for treating dyszoospermia. The extract provided by the invention is prepared from roxburgh rose fruits or roxburgh rose pomace through extraction and enrichment, the raw material resources are rich, the price is low, and the toxic and side effects are low. Animal experiments show that the roxburgh rose active extract has the activity of improving dyszoospermia, and the roxburgh rose active extract can obviously increase the sperm concentration and motility rate of mice with dyszoospermia, reduce the malformation rate and obviously increase the thickness of spermatogenic epithelium, the number of supporting cells and the number of interstitial cells of testicular tissues of the mice with dyszoospermia. WB detection analysis shows that the action way of improving the dyszoospermia by the roxburgh rose active extract is to inhibit activation of an oxidative stress Keap1 / Nrf2 signal channel. The rosa roxburghii tratt active extract has a new function of improving dyszoospermia and can be applied as a medicine for improving dyszoospermia.

The invention relates to application of SMAD6 in auxiliary diagnosis and prognosis evaluation of TGCT (Transforming Growth Computed Tomography). According to the invention, a novel TGCT prognosis biomarker (SMAD6) is identified through difference analysis, Cox regression and survival analysis on the basis of a TGCT sample in multiple centers. Subsequently, the expression level of SMAD6 in normal testicular tissues and TGCT is evaluated through immunohistochemical staining. Finally, the relationship between the SMAD6 and biological characteristics, mutation landscape, immune cell infiltration and immunotherapy response is also studied. The invention defines that SMAD6 is a risk factor of poor TGCT prognosis. An immunohistochemical result shows that the SMAD6 is highly expressed in the TGCT tissue. GSEA shows that SMAD6 is related to activation of tumor-related pathways and inhibition of immune-related pathways. In addition, high SMAD6 expression is associated with lower CD8 + T cell infiltration, while patients with low SMAD6 expression gain more benefits in immunotherapy. The invention fully reveals the potential of SMAD6 as a TGCT prognosis and immunotherapy reaction biomarker, and provides a new scientific basis and theoretical basis for personalized treatment of TGCT.

The invention discloses a lactobacillus gasseri strain capable of improving memory and promoting growth and a metagen thereof, and belongs to the technical field of microbial fermentation. A strain of lactobacillus gasseri is obtained through screening, thalli and metabiotics of the thalli are obtained through culture, the metabiotics and the reed rhizome extract form the compound, and the compound shows a synergistic interaction effect which is specifically shown as follows: in a water maze test, the incubation period of a mouse is shortened; in a rat growth test, the body length, the body width and the testis weight of the rat are all improved. The lactobacillus gasseri and the preparation method thereof have practical value on comprehensive utilization of lactobacillus gasseri and the metaplasts thereof, and also have important value in the aspects of improving memory of an organism and promoting growth.

The present disclosure relates to methods for characterizing cancer testis antigen burden (“CTAB”), for predicting cancer survival outcomes using CTAB analysis, and for recommending and / or treating cancer using CTAB analysis. Particularly, aspects are directed to measuring expression of a panel of cancer testis antigen (CTA) gene markers in a tumor sample from a subject, determining a CTAB based on the measured expression of the CTA gene markers, classifying, by comparing to a reference CTAB score from the same cancer or tumor type, the CTAB score as a high-CTAB score or a low-CTAB score, and identifying the subject as responsive to a treatment wherein (i) the high-CTAB score is indicative of responsiveness to the treatment, or (ii) the low-CTAB score is indicative of responsiveness to the treatment.

The invention discloses an application of uridine in promotion of injury regeneration or proliferation of spermatogonial stem cells, and discloses a new application of uridine in promotion of injury regeneration or proliferation of spermatogonial stem cells for the first time. The same effect is also achieved in the aspects of promoting proliferation of spermatogonial stem cells cultured in vitro, spermatogonial stem cells in testis organs cultured in vitro and spermatogonial stem cells in senescent testis, and the problem that the number of spermatogonial stem cells is reduced due to testis injury caused by chemotherapeutic drugs can be solved; therefore, a new solution is provided for clinical treatment of male infertility and male fertility protection.

The invention discloses novel application of a GJB5 gene, a construction method of a GJB5 gene overexpression vector and a method for improving high temperature resistance of economic animals, and relates to the technical field of molecular biology. The preparation for overexpressing the GJB5 gene improves the heat resistance of the economic animal sertoli cells through overexpression of the GJB5 gene. Meanwhile, the invention also discloses a construction method of the GJB5 gene overexpression vector and a method for improving the high temperature resistance of economic animals. The effect of the GJB5 gene in heat stress is found for the first time, the gene is expected to be overexpressed in pigs or genetically modified into economic animals such as cattle and sheep in the future, so that the heat resistance of the pigs is improved, the semen quality of the pigs under the heat stress condition is further improved, and the GJB5 gene has important social benefits and economic benefits.

The invention discloses construction of a Guangdong small-ear spotted pig spermatogonial stem cell in-vitro culture system and screening of surface markers, and belongs to the technical field of cell culture.The technical scheme includes that testis are broken firstly, then enzyme digestion, density gradient centrifugal purification and differential adherent purification are conducted, and purified spermatogonial stem cells are obtained; inoculating the obtained spermatogonial stem cells to feeder layer cells and culturing in a culture medium; the culture medium is a DMEM culture medium added with FBS and growth factors; the mass fraction of the FBS in the DMEM culture medium is 8-12%, an enzyme digestion method, a density gradient centrifugation method and a differential adhesion method are combined for application, the Guangdong small-ear spotted pig spermatogonia stem cells with certain purity are obtained, cells enriched and purified in vitro can proliferate massively, and an in-vitro culture system of the Guangdong small-ear spotted pig spermatogonia stem cells is established.

The invention relates to the technical field of biological medicine, and discloses rapamycin-loaded microemulsion gel as well as a preparation method and application thereof. The preparation method of the microemulsion gel comprises the following steps: dissolving N, N-dimethyl octadecyl carboxymethyl chitosan in a water phase, then adding an oil phase, a cosurfactant and rapamycin, and stirring to obtain rapamycin-loaded microemulsion; the preparation method comprises the following steps: dissolving a high polymer material in a PBS solution, heating until the high polymer material is completely dissolved, then adding dopamine, stirring for reaction, and finally dialyzing and freeze-drying to obtain the adhesive. And adding a gel matrix containing a thickening agent and an adhesive into the microemulsion, and stirring to obtain the rapamycin-loaded microemulsion gel. According to the prepared microemulsion gel, transdermal drug delivery of hydrophobic drugs is achieved, testis are coated with the microemulsion gel loaded with rapamycin, the blocking effect of damp-heat stress on differentiation and development of testis interstitial cells can be relieved, and the functionality of a male reproduction endocrine system is recovered.

The invention relates to a real-time testis rare sperm auxiliary identification method and device which are used for testis incision sperm extraction under a microscope, and the method comprises the following steps: obtaining first real-time image dynamic data; inputting the first real-time image dynamic data to a rare testis sperm auxiliary identification model to obtain rare testis sperm data; and updating the first real-time image dynamic data based on the rare testis sperm data to obtain second real-time image dynamic data. The method has the advantages that due to the fact that the auxiliary identification model for the rare sperms of the testis is high in response speed, the first real-time image dynamic data can be rapidly identified and updated, and the problems of labeling dislocation, repeated identification and the like cannot occur.

The invention discloses an IQUB gene molecular marker related to the sizes of Hu sheep testis and epididymis and application of the molecular marker. The molecular marker is obtained by amplifying an IQUB gene sequence through a designed specific primer, the nucleotide sequence of the molecular marker is shown as SEQ ID NO.1 in a sequence table, the 147bp position of the SEQ ID NO.1 in the sequence table has base mutation of A / C, and the mutation causes Mlu I-RFLP enzyme digestion polymorphism. By analyzing the correlation between Hu sheep IQUB genotyping and testis and epididymis sizes, the result shows that genotypes of Hu sheep individuals are divided into an AA genotype, an AC genotype and a CC genotype, and the testis weight and epididymis total weight of the AC genotype individuals are obviously higher than those of the AA genotype individuals and those of the CC genotype individuals. The molecular marker is used for Hu sheep seed selection, the accuracy is high, Hu sheep individuals with high reproductive capacity can be screened out when hu sheep rams are young, early breeding of the Hu sheep variety is achieved, and therefore the breeding process is accelerated.

The invention relates to the technical field of cell culture media, in particular to a sheep testis somatic cell culture medium and a culture method.The sheep testis somatic cell culture medium is prepared from, by weight, 10-15 parts of MEMS alpha, 4-7 parts of KSR, 2-4 parts of sodium chloride, 3-5 parts of recombinant human transferrin, 1-3 parts of ferric citrate, 2-4 parts of L-glutamine, 5-10 parts of water and 4-7 parts of hydroxyapatite. According to the cell culture medium, MEM and KSR are adopted as matrix raw materials, sodium chloride and other auxiliaries are added, the hydroxyapatite agent is matched, the performance change of the cell culture medium on the survival rate and the survival amount of testicular somatic cells is enhanced, meanwhile, hydroxyapatite serves as a matrix of the hydroxyapatite agent, the performance of the cell culture medium is improved through a specific modification solution, and the cell culture medium is prepared. And the function of hydroxyapatite blended kaolin in the system is optimized, so that the functional effect of the cell culture medium is enhanced, and the performance of the product is further improved.

The invention discloses application of semen cuscutae-fructus lycii in preparation of a medicine for regulating and controlling an SIRT1 / Nrf2 signal channel to improve oligoasthenospermia, and belongs to the technical field of biology. According to the application of the semen cuscutae-fructus lycii in preparing the medicine for regulating and controlling the SIRT1 / Nrf2 signal channel to improve the oligoasthenospermia, disclosed by the invention, the semen cuscutae-fructus lycii regulates and controls the SIRT1 / Nrf2 expression of testicular tissues, enhances the anti-oxidative stress capability, up-regulates the BTB related protein expression level and repairs BTB damage, so that the spermatogenic function of OAS rats is improved, and the effect of improving the oligoasthenospermia is achieved. And the total flavonoids can be a material basis for playing a drug treatment effect. A powerful experimental support is provided for research on a mechanism of treating OAS by exerting the anti-oxidative stress effect of the semen cuscutae-fructus lycii, and a theoretical basis is provided for research on Ningxia genuine medicinal materials.

The present disclosure provides an iterative process for identifying culture conditions that maintain identity, growth, and survival of testicular cells in vitro. Testicular cell culturing systems for supporting human spermatogenesis and culture using identified culture conditions are also provided. The methods and the culture conditions can be used to culture healthy and viable spermatozoa with lower rates of deleterious or de novo mutations or epigenetic perturbations from fertile and infertile men for future use with assisted reproductive technologies.

This invention provides a biomarker for spermatogenesis disorders and its application, wherein the biomarker is the MNDA molecule. This application characterizes the functional abnormalities or spermatogenesis disorders of Sertoli cells by detecting the expression level of MNDA molecules on cells in testicular tissue sections, and can therefore be used for the clinical diagnosis of non-obstructive azoospermia.

The invention relates to a method for the in vitro determination of the immunogenicity of a first antigen, which is referred to as the test antigen, wherein the method comprises: (a) Selecting at least one first molecule by incubating the test antigen with a mixture of molecules containing the at least one first molecule and at least one second molecule to enable the adsorption of the at least one first molecule or of the at least one second molecule onto the test antigen, (b) Performing a neutralization test using a second antigen, referred to as the target antigen, and using at least one first molecule selected in step (a), obtaining a neutralization titer; and (c) Determining the immunogenicity of the test antigen using the neutralization titer obtained in step (b).

The invention discloses application of combination of ginsenoside Rb1 and luteolin in prevention and treatment of male reproductive toxicity caused by organophosphorus pesticides, and belongs to the field of medicines. A high-toxicity organophosphorus pesticide glyphosate is used as a representative, the reproductive toxicity effect of the glyphosate on male mice and the relieving effect of the ginsenoside Rb1 and the composition of the ginsenoside Rb1 and the luteolin on the male reproductive toxicity of the glyphosate are observed through tests, and the result shows that the ginsenoside Rb1 and the luteolin composition have the effect of inhibiting the reproductive toxicity of the glyphosate. The ginsenoside Rb1 and the composition of the ginsenoside Rb1 and the luteolin have a remarkable relieving effect on male reproductive toxic diseases caused by organophosphorus pesticides, wherein the male reproductive toxic diseases comprise testis diseases and sperm malformation rate increase, and antioxidant index GSH decrease and T-SOD increase caused by the organophosphorus pesticides. Effective drugs and treatment strategies are provided for preventing and treating organophosphorus pesticide induced male reproductive toxicity and diseases induced by organophosphorus pesticide induced male reproductive toxicity.

The invention belongs to the technical field of biological medicines, and particularly discloses application of roxburgh rose glycoside in preparation of a medicine for improving dyszoospermia. Pharmacodynamic verification is carried out by constructing various animal models with dyszoospermia, and it is found that the roxburgh rose glycoside can significantly improve reproductive function indexes, for example, the roxburgh rose glycoside can significantly increase the density of testicular interstitial cells, the thickness of spermatogenic epithelium and the density of sperms in epididymis lumens. As a main active component of a medicinal and edible plant roxburgh rose, the roxburgh rose glycoside has good biological safety and human body tolerance. The new application of the roxburgh rose glycoside in the field of reproductive medicine is disclosed for the first time, a novel candidate medicine is provided for treating dyszoospermia, and meanwhile, an application system of the roxburgh rose glycoside in functional health care products and healthy food additives is constructed.

This invention relates to the finding that testis development related protein (TDRP; also termed Immuno-moodulin or Imood herein) is a circulatory anxiogenic factor that modulates anxiety-like behaviour in mammalian models through the regulation of the immune system. Methods of treatment of mental disorders, such as anxiety, and TDRP antagonists for use in such methods are provided. Methods of diagnosing or monitoring a mental disorder in an individual by determining levels of TDRP in a sample and methods of screening for compounds that reduce levels of TDRP are provided.

The application belongs to the technical field of biological pharmacy, and particularly relates to a bovine-derived mouse anti-Brucella antibody and a preparation method and application thereof. A bovine-derived mouse anti-Brucella antibody is prepared by synthesizing a tandem epitope protein of Brucella OMP25 proteins of bovine species, sheep testis species and canine species, immunizing mice, preparing a monoclonal antibody, cloning CDR fragments in Hv and Lv regions of the antibody to corresponding positions of bovine-derived IgG, and then performing overall mutation modification on the recombinant IgG to perform bovine-derived modification. The bovine-derived mouse anti-Brucella antibody preparation has good immunogenicity and protection effect, and can block and inhibit the propagation and spread of Brucella of bovine species, canine species and sheep testis species in an animal body.

The invention discloses a reagent for editing a pig INSL3 gene and application of the reagent. The reagent for editing the pig INSL3 gene contains an INSL3-sgRNA A and an INSL3-sgRNA B, wherein the INSL3-sgRNA A and the INSL3-sgRNA The target sequence of the INSL3-sgRNA A is as shown in SEQ ID No.5; the target sequence of the INSL3-sgRNA B is shown in SEQ ID No. 6. Experiments prove that the INSL3-sgRNA A and the INSL3-sgRNA B are combined with a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 system, so that the pig INSL3 gene can be efficiently edited, and the editing efficiency of the INSL3 gene of pig testicular cells and the editing efficiency of the INSL3 gene of pig fetal fibroblasts can reach 8.47% and 11.45% respectively. The invention has important significance on the function research of the INSL3 gene of pig cells or individuals and the breeding of pig genetic disease resistance.