187 results about "Mouse Testis" patented technology

The invention relates to the technical field of biology, in particular to multifunctional belly-expanding hippocampal peptide as well as a preparation method and application thereof. Comprising a polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The hippocampus antioxidative peptide is prepared by adopting an ultrahigh pressure pretreatment and enzymolysis technology, the adopted extraction process is simple in route, and the time and the production cost are effectively saved; an animal model is utilized to prove the anti-oxidation effect and the effect of improving male reproductive disorder and testis injury of the multifunctional belly-expanding hippocampal peptide; and on the basis, a novel peptide fragment with high activity, no toxicity and no sensitization is obtained.

The invention relates to the technical field of biological medicines, in particular to an umbilical cord mesenchymal stem cell exosome and application of the umbilical cord mesenchymal stem cell exosome in preparation of a medicine for treating male infertility caused by hidden testis. The prepared high-concentration umbilical cord mesenchymal stem cell exosome suspension preparation is directly used for treating cultured spermatogonial cells and spermatoblasts in vitro, is injected into the testis of a mouse, and is used for improving the seminiferous tubule and spermatogenic microenvironment of testis tissue and promoting division and proliferation of the spermatogonial cells and the spermatoblasts. The method can effectively reverse oligospermia and azoospermia caused by the hidden testis, and compared with a model without intervention of treatment means, the method can effectively promote increase and even regeneration of sperms in the testis.

The invention discloses a traditional Chinese medicine compound oral preparation for treating male oligoasthenospermia, and belongs to the technical field of traditional Chinese medicine preparations. The rhizoma polygonati processed with wine, the prepared rehmannia root and the like are used for nourishing the liver and kidney to consolidate congenital, the codonopsis pilosula, the astragalus membranaceus and the like are used for invigorating spleen and replenishing qi to complement postnatal, the fried cowherb seed is used for activating blood circulation, and the salted semen plantaginis is used for promoting diuresis, so that the problems of insufficient spermatogenesis and low vitality caused by deficiency of the spleen and the kidney are fundamentally solved. Experiments and clinical researches show that the preparation can significantly improve the sperm quantity, activity and activity rate of oligoasthenospermia model mice and patients, the clinical total effective rate reaches 90%, the pregnancy rate reaches 53.3%, and the curative effect is superior to that of Wuzi Yanzong pills in a control group. The action mechanism covers regulation of blood lipid metabolism, improvement of hypothalamus-pituitary-gonad axis functions, repair of testicular seminiferous tubule structural damage and reduction of lipid deposition, and meanwhile, oxidative stress and inflammatory injury are relieved and spermatogenic cell functions are protected by regulating and controlling a PPAR signal channel and inhibiting molecular pathways such as Cga / Sting mediated cell apoptosis.

The invention provides application of a CD47 gene in detection of pig litter size phenotype and application of an SNP site of the CD47 gene in gene expression regulation. It is found that the CD47 gene is regulated and controlled by the SNP site rs323354626, it is further found that the rs323354626 site and the phenotype of the total litter size of sows show the highest significance, and it is indicated that the CD47 gene and the total litter size of the sows have large correlation. In three main commercial varieties of pigs, namely Duroc, Landrace and Yorkshire, the CD47 expression level in testis tissues is negatively correlated with the litter size, which indicates that the expression mode of the CD47 gene may influence the reproductive performance of the pigs, and the litter size phenotype of the pigs can be detected by detecting the CD47 gene expression level in the testis of the breeding pigs or somatic cells of the sows. Or the litter size of the pigs is influenced by regulating and controlling the expression level of the CD47 gene through the rs323354626 site, and the discovery lays a foundation for improving the breeding efficiency of the pigs, especially for a breeding method for increasing the litter size.

The invention relates to the technical field of biology, in particular to application of rosa roxburghii tratt active extract in preparation of a medicine for treating dyszoospermia. The extract provided by the invention is prepared from roxburgh rose fruits or roxburgh rose pomace through extraction and enrichment, the raw material resources are rich, the price is low, and the toxic and side effects are low. Animal experiments show that the roxburgh rose active extract has the activity of improving dyszoospermia, and the roxburgh rose active extract can obviously increase the sperm concentration and motility rate of mice with dyszoospermia, reduce the malformation rate and obviously increase the thickness of spermatogenic epithelium, the number of supporting cells and the number of interstitial cells of testicular tissues of the mice with dyszoospermia. WB detection analysis shows that the action way of improving the dyszoospermia by the roxburgh rose active extract is to inhibit activation of an oxidative stress Keap1 / Nrf2 signal channel. The rosa roxburghii tratt active extract has a new function of improving dyszoospermia and can be applied as a medicine for improving dyszoospermia.

The invention discloses a lactobacillus gasseri strain capable of improving memory and promoting growth and a metagen thereof, and belongs to the technical field of microbial fermentation. A strain of lactobacillus gasseri is obtained through screening, thalli and metabiotics of the thalli are obtained through culture, the metabiotics and the reed rhizome extract form the compound, and the compound shows a synergistic interaction effect which is specifically shown as follows: in a water maze test, the incubation period of a mouse is shortened; in a rat growth test, the body length, the body width and the testis weight of the rat are all improved. The lactobacillus gasseri and the preparation method thereof have practical value on comprehensive utilization of lactobacillus gasseri and the metaplasts thereof, and also have important value in the aspects of improving memory of an organism and promoting growth.

The invention discloses novel application of a GJB5 gene, a construction method of a GJB5 gene overexpression vector and a method for improving high temperature resistance of economic animals, and relates to the technical field of molecular biology. The preparation for overexpressing the GJB5 gene improves the heat resistance of the economic animal sertoli cells through overexpression of the GJB5 gene. Meanwhile, the invention also discloses a construction method of the GJB5 gene overexpression vector and a method for improving the high temperature resistance of economic animals. The effect of the GJB5 gene in heat stress is found for the first time, the gene is expected to be overexpressed in pigs or genetically modified into economic animals such as cattle and sheep in the future, so that the heat resistance of the pigs is improved, the semen quality of the pigs under the heat stress condition is further improved, and the GJB5 gene has important social benefits and economic benefits.

The invention discloses construction of a Guangdong small-ear spotted pig spermatogonial stem cell in-vitro culture system and screening of surface markers, and belongs to the technical field of cell culture.The technical scheme includes that testis are broken firstly, then enzyme digestion, density gradient centrifugal purification and differential adherent purification are conducted, and purified spermatogonial stem cells are obtained; inoculating the obtained spermatogonial stem cells to feeder layer cells and culturing in a culture medium; the culture medium is a DMEM culture medium added with FBS and growth factors; the mass fraction of the FBS in the DMEM culture medium is 8-12%, an enzyme digestion method, a density gradient centrifugation method and a differential adhesion method are combined for application, the Guangdong small-ear spotted pig spermatogonia stem cells with certain purity are obtained, cells enriched and purified in vitro can proliferate massively, and an in-vitro culture system of the Guangdong small-ear spotted pig spermatogonia stem cells is established.

The invention relates to the technical field of biological medicine, and discloses rapamycin-loaded microemulsion gel as well as a preparation method and application thereof. The preparation method of the microemulsion gel comprises the following steps: dissolving N, N-dimethyl octadecyl carboxymethyl chitosan in a water phase, then adding an oil phase, a cosurfactant and rapamycin, and stirring to obtain rapamycin-loaded microemulsion; the preparation method comprises the following steps: dissolving a high polymer material in a PBS solution, heating until the high polymer material is completely dissolved, then adding dopamine, stirring for reaction, and finally dialyzing and freeze-drying to obtain the adhesive. And adding a gel matrix containing a thickening agent and an adhesive into the microemulsion, and stirring to obtain the rapamycin-loaded microemulsion gel. According to the prepared microemulsion gel, transdermal drug delivery of hydrophobic drugs is achieved, testis are coated with the microemulsion gel loaded with rapamycin, the blocking effect of damp-heat stress on differentiation and development of testis interstitial cells can be relieved, and the functionality of a male reproduction endocrine system is recovered.

The invention relates to a real-time testis rare sperm auxiliary identification method and device which are used for testis incision sperm extraction under a microscope, and the method comprises the following steps: obtaining first real-time image dynamic data; inputting the first real-time image dynamic data to a rare testis sperm auxiliary identification model to obtain rare testis sperm data; and updating the first real-time image dynamic data based on the rare testis sperm data to obtain second real-time image dynamic data. The method has the advantages that due to the fact that the auxiliary identification model for the rare sperms of the testis is high in response speed, the first real-time image dynamic data can be rapidly identified and updated, and the problems of labeling dislocation, repeated identification and the like cannot occur.

The invention discloses an IQUB gene molecular marker related to the sizes of Hu sheep testis and epididymis and application of the molecular marker. The molecular marker is obtained by amplifying an IQUB gene sequence through a designed specific primer, the nucleotide sequence of the molecular marker is shown as SEQ ID NO.1 in a sequence table, the 147bp position of the SEQ ID NO.1 in the sequence table has base mutation of A / C, and the mutation causes Mlu I-RFLP enzyme digestion polymorphism. By analyzing the correlation between Hu sheep IQUB genotyping and testis and epididymis sizes, the result shows that genotypes of Hu sheep individuals are divided into an AA genotype, an AC genotype and a CC genotype, and the testis weight and epididymis total weight of the AC genotype individuals are obviously higher than those of the AA genotype individuals and those of the CC genotype individuals. The molecular marker is used for Hu sheep seed selection, the accuracy is high, Hu sheep individuals with high reproductive capacity can be screened out when hu sheep rams are young, early breeding of the Hu sheep variety is achieved, and therefore the breeding process is accelerated.

The invention relates to the technical field of cell culture media, in particular to a sheep testis somatic cell culture medium and a culture method.The sheep testis somatic cell culture medium is prepared from, by weight, 10-15 parts of MEMS alpha, 4-7 parts of KSR, 2-4 parts of sodium chloride, 3-5 parts of recombinant human transferrin, 1-3 parts of ferric citrate, 2-4 parts of L-glutamine, 5-10 parts of water and 4-7 parts of hydroxyapatite. According to the cell culture medium, MEM and KSR are adopted as matrix raw materials, sodium chloride and other auxiliaries are added, the hydroxyapatite agent is matched, the performance change of the cell culture medium on the survival rate and the survival amount of testicular somatic cells is enhanced, meanwhile, hydroxyapatite serves as a matrix of the hydroxyapatite agent, the performance of the cell culture medium is improved through a specific modification solution, and the cell culture medium is prepared. And the function of hydroxyapatite blended kaolin in the system is optimized, so that the functional effect of the cell culture medium is enhanced, and the performance of the product is further improved.

This invention provides a biomarker for spermatogenesis disorders and its application, wherein the biomarker is the MNDA molecule. This application characterizes the functional abnormalities or spermatogenesis disorders of Sertoli cells by detecting the expression level of MNDA molecules on cells in testicular tissue sections, and can therefore be used for the clinical diagnosis of non-obstructive azoospermia.

The invention relates to a method for the in vitro determination of the immunogenicity of a first antigen, which is referred to as the test antigen, wherein the method comprises: (a) Selecting at least one first molecule by incubating the test antigen with a mixture of molecules containing the at least one first molecule and at least one second molecule to enable the adsorption of the at least one first molecule or of the at least one second molecule onto the test antigen, (b) Performing a neutralization test using a second antigen, referred to as the target antigen, and using at least one first molecule selected in step (a), obtaining a neutralization titer; and (c) Determining the immunogenicity of the test antigen using the neutralization titer obtained in step (b).

The invention discloses application of combination of ginsenoside Rb1 and luteolin in prevention and treatment of male reproductive toxicity caused by organophosphorus pesticides, and belongs to the field of medicines. A high-toxicity organophosphorus pesticide glyphosate is used as a representative, the reproductive toxicity effect of the glyphosate on male mice and the relieving effect of the ginsenoside Rb1 and the composition of the ginsenoside Rb1 and the luteolin on the male reproductive toxicity of the glyphosate are observed through tests, and the result shows that the ginsenoside Rb1 and the luteolin composition have the effect of inhibiting the reproductive toxicity of the glyphosate. The ginsenoside Rb1 and the composition of the ginsenoside Rb1 and the luteolin have a remarkable relieving effect on male reproductive toxic diseases caused by organophosphorus pesticides, wherein the male reproductive toxic diseases comprise testis diseases and sperm malformation rate increase, and antioxidant index GSH decrease and T-SOD increase caused by the organophosphorus pesticides. Effective drugs and treatment strategies are provided for preventing and treating organophosphorus pesticide induced male reproductive toxicity and diseases induced by organophosphorus pesticide induced male reproductive toxicity.

The invention belongs to the technical field of biological medicines, and particularly discloses application of roxburgh rose glycoside in preparation of a medicine for improving dyszoospermia. Pharmacodynamic verification is carried out by constructing various animal models with dyszoospermia, and it is found that the roxburgh rose glycoside can significantly improve reproductive function indexes, for example, the roxburgh rose glycoside can significantly increase the density of testicular interstitial cells, the thickness of spermatogenic epithelium and the density of sperms in epididymis lumens. As a main active component of a medicinal and edible plant roxburgh rose, the roxburgh rose glycoside has good biological safety and human body tolerance. The new application of the roxburgh rose glycoside in the field of reproductive medicine is disclosed for the first time, a novel candidate medicine is provided for treating dyszoospermia, and meanwhile, an application system of the roxburgh rose glycoside in functional health care products and healthy food additives is constructed.

This invention relates to the finding that testis development related protein (TDRP; also termed Immuno-moodulin or Imood herein) is a circulatory anxiogenic factor that modulates anxiety-like behaviour in mammalian models through the regulation of the immune system. Methods of treatment of mental disorders, such as anxiety, and TDRP antagonists for use in such methods are provided. Methods of diagnosing or monitoring a mental disorder in an individual by determining levels of TDRP in a sample and methods of screening for compounds that reduce levels of TDRP are provided.

The application belongs to the technical field of biological pharmacy, and particularly relates to a bovine-derived mouse anti-Brucella antibody and a preparation method and application thereof. A bovine-derived mouse anti-Brucella antibody is prepared by synthesizing a tandem epitope protein of Brucella OMP25 proteins of bovine species, sheep testis species and canine species, immunizing mice, preparing a monoclonal antibody, cloning CDR fragments in Hv and Lv regions of the antibody to corresponding positions of bovine-derived IgG, and then performing overall mutation modification on the recombinant IgG to perform bovine-derived modification. The bovine-derived mouse anti-Brucella antibody preparation has good immunogenicity and protection effect, and can block and inhibit the propagation and spread of Brucella of bovine species, canine species and sheep testis species in an animal body.

The invention discloses a reagent for editing a pig INSL3 gene and application of the reagent. The reagent for editing the pig INSL3 gene contains an INSL3-sgRNA A and an INSL3-sgRNA B, wherein the INSL3-sgRNA A and the INSL3-sgRNA The target sequence of the INSL3-sgRNA A is as shown in SEQ ID No.5; the target sequence of the INSL3-sgRNA B is shown in SEQ ID No. 6. Experiments prove that the INSL3-sgRNA A and the INSL3-sgRNA B are combined with a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 system, so that the pig INSL3 gene can be efficiently edited, and the editing efficiency of the INSL3 gene of pig testicular cells and the editing efficiency of the INSL3 gene of pig fetal fibroblasts can reach 8.47% and 11.45% respectively. The invention has important significance on the function research of the INSL3 gene of pig cells or individuals and the breeding of pig genetic disease resistance.

Tumor-specific T cell receptor (TCR) gene transfer enables specific and potent immune targeting of tumor antigens. The canonical cancer-testis antigen, NY-ESO-1, is not expressed in normal tissues but is aberrantly expressed across a broad array of cancer types. It has also been targeted with A2-restricted TCR gene therapy without adverse events or notable side effects. To enable the targeting of NY-ESO-1 in a broader array of HLA haplotypes, we isolated TCRs specific for NY-ESO-1 epitopes presented by four MHC molecules: HLA-A2, -B07, -B18, and -C03. Using these TCRs, we have developed an approach to extend TCR gene therapies targeting NY-ESO-1 to patient populations beyond those expressing HLA-A2.

The invention discloses a pilose antler active peptide and application thereof in anti-inflammation, anti-oxidation and anti-aging, and belongs to the technical field of biology. According to the invention, the brand-new active peptide SPLASDLDL screened out from pilose antler for the first time is utilized, and competitive inhibition on TNFR1 is realized through the characteristic that the brand-new active peptide SPLASDLDL is specifically combined with a TNFR1 receptor. A constructed sertoli cell senescence model proves that the polypeptide has anti-inflammatory, anti-oxidation and anti-senescence effects. The invention successfully solves the technical problem that in the prior art, the competitive inhibition TNFR1 targeting peptide cannot be found from pilose antler and the action mechanism of the TNFR1 targeting peptide cannot be clarified, and provides the action mechanism and scientific basis for developing novel anti-inflammatory and anti-aging drugs derived from natural products.

The invention discloses application of exosome-like nanoparticles derived from fructus lycii to preparation of a medicine for preventing or treating oligospermia and / or asthenospermia. Sterile, stable and high-purity fructus lycii exosome-like nanoparticles (LBELNs) are obtained by adopting a differential centrifugation-suction filtration-ultra-high-speed centrifugation method, the physiological efficacy of the LBELNs is researched, and it is found that the LBELNs can significantly improve the oligoasthenospermia disease activity index of male mice induced by cyclophosphamide (CTX); the sperm quantity is obviously increased, the sperm malformation is improved, the sperm motility is improved, and the sex hormone level is recovered. The LBELNs can effectively relieve spermatogenic dysfunction of testicular tissues, the sperm differentiation and formation process is obviously improved, the total number of sperms is increased, and the treatment effect is more obvious along with increase of the dosage of the LBELNs. The invention has potential in preparation of drugs for preventing or treating oligoasthenospermia, and provides a new strategy and treatment method for treating oligoasthenospermia.

The invention relates to the technical field of lion-head goose breeding, in particular to a method for screening and identifying genes related to lion-head goose sperm motility regulation and control, which comprises the following steps: dividing healthy lion-head male geese in a breeding period into a high sperm motility group and a low sperm motility group according to sperm motility, collecting two groups of testicular tissues and sperm samples, and preserving the testicular tissues at low temperature for molecular detection; performing transcriptome sequencing on the testicular tissue to obtain mRNA and non-coding RNA expression data; screening two groups of nucleic acid molecules with significant differential expression, and determining candidate genes through functional enrichment analysis of reproductive development related biological pathways; and verifying the authenticity of the expression difference of the candidate gene through fluorescent quantitative PCR, and finally determining the target gene. According to the method, related data are obtained through sequencing, the candidate genes are determined by screening differential molecules through enrichment analysis and PCR verification, accurate identification of a related gene system is achieved, and the problems of missing detection and insufficient accuracy caused by lack of transcriptome sequencing in a traditional method are solved.

The invention discloses a testis Leydig cell senescence marker and application thereof, the senescence marker is CCN5 protein, the senescence condition of the Leydig cell can be specifically reflected by detecting the CCN5 level of testis tissue, the senescence marker can be used as an index for evaluating Leydig cell senescence and testis senescence, and meanwhile, the senescence marker is an effective supplement for a diagnostic scheme of late onset hypogonadism (LOH). Furthermore, the invention also provides application of a preparation for inhibiting CCN5 protein expression in preparation of a product for improving testis Leydig cell senescence or treating diseases caused by testis Leydig cell senescence, the testis Leydig cell senescence can be improved and the LOH symptom can be treated by reducing CCN5 in a targeted manner, and treatment can be performed aiming at the core phenotypes of'lipid droplet loss' and'fibrosis' of testis Leydig cell senescence. The method is a treatment scheme different from exogenous testosterone supplement, stem cell injection, Senlytics drugs and exosome therapy.

The present disclosure provides bovine animals and methods to create recipient bovine animals for spermatogenic stem cell transplantation / complementation through modulation of the NANOS2 gene. In one embodiment genome editing was used to create bovine animals with insertions or deletions (indels) that inactivate or otherwise modulate NANOS2 gene activity so that resulting male bovines lack functional germ cells yet retain functional testicular somatic cells, and bovine females are fertile. These bovine males can then be transplanted or complemented with donor cells capable of giving rise to cells of the spermatogenic lineage, or spermatogenic stem cells and used for breeding.

The application discloses an immunogen for preparing testis-specific glyceraldehyde-3-phosphate dehydrogenase lysine malonylation site-specific antibody, and belongs to the technical field of biotechnology.The immunogen is a malonylation modified polypeptide, and the amino acid sequence is IIPASTGAAKAVT, corresponding to the 278th-290th amino acid of a human GAPDHS protein, wherein a lysine at the 287th position (the 10th position in the sequence) is subjected to malonylation modification.A specific antibody designed according to the immunogen has the characteristics of high titer and high specificity. By screening a human sperm sample by using the antibody, it is found that the antibody can not only specifically recognize the GAPDHS with the lysine at the 287th position subjected to malonylation modification in vitro and in human sperm, but also is closely related to sperm forward movement and asthenozoospermia. The application has great significance in the field of preparation of reagents for precision diagnosis and treatment of idiopathic male infertility.

This invention discloses a Fam205a1 Methods for constructing and applying gene knockout mouse models. The construction method utilizes CRISPR / Cas9 gene editing technology, targeting mice. Fam205a1 Gene-designed specific sgRNAs for knockout Fam205a1 The regions of exons 1 through 4 of the gene are obtained. Fam205a1 Gene knockout mice. Male mice in this model exhibit infertility, specifically characterized by impaired spermatogenesis, reduced sperm motility, abnormal sperm morphology (particularly head and acrosome defects), and spermatogenic cell apoptosis. However, the weight and appearance of their testes and epididymis show no significant difference from the wild type. The mouse model constructed in this invention has stable genetic traits and a well-defined phenotype, making it an ideal animal model for studying the mechanisms of idiopathic male infertility, screening drugs for treating male infertility, or developing male contraceptives.

The invention relates to the technical field of genetic engineering, in particular to application of PCK1 in preparation of products for regulating and controlling goat testicular interstitial cell proliferation. According to the invention, an over-expression plasmid pcDNA3.1 (+)-PCK1 of the PCK1 gene is constructed, an interference sequence si-PCK1 of the PCK1 gene is synthesized, and it is proposed for the first time that the over-expression of the PCK1 gene can significantly promote the proliferation of goat testicular interstitial cells, and the inhibition of the expression of the PCK1 gene can significantly inhibit the proliferation of the goat testicular interstitial cells. The invention has important significance for improving the development of the goat testis, and provides technical support for researching and developing related products for regulating and controlling the proliferation of the goat testis interstitial cells by taking the PCK1 gene as the target spot.

An embodiment relates to polydeoxyribonucleotide (PDRN)-containing exosomes, a composition including same, and a preparation method therefor, wherein the PDRN-containing exosomes of the embodiment are derived from a fish testis tissue and include high-purity, low-molecular weight PDRN. In addition, exosomes containing a significant amount of low molecular weight PDRN can be induced through a simple process of ultrasonic treatment. Furthermore, application of ultrasound to the separated exosomes can prepare PDRN exosomes that are made to have low molecular weights, whereby the exosomes exhibit high intracellular uptake rates and thus can be advantageously used for manufacturing a tissue regeneration or anti-inflammatory composition.

The invention discloses preparation and application of a mouse TMC5 truncated recombinant protein and a polyclonal antibody, and belongs to the technical field of biological medicine. The amino acid sequence of the TMC5 truncated recombinant protein is as shown in SEQ ID NO. 1, and the nucleotide sequence of the TMC5 truncated recombinant protein is as shown in SEQ ID NO. 2. Compared with a full-length protein, the mouse TMC5 truncated recombinant protein provided by the invention has better stability and antigenicity, the obtained polyclonal antibody is high in titer and strong in specificity, the identification of endogenous TMC5 protein in mouse testicular tissues is facilitated, meanwhile, the TMC5 truncated recombinant protein and the polyclonal antibody can also be used for researching a mechanism of regulating spermatogenesis by TMC5, and the application prospect is broad. The blank of preparation of the mouse TMC5 antibody and functional research of the mouse TMC5 antibody in the testis is filled. The mouse TMC5 truncated recombinant protein and the polyclonal antibody can be applied to development of novel diagnostic reagents and drugs for treating male sterility.