11 results about "Genetic screening method" patented technology

The application discloses a kind of screening methods and application of pivot gene related to sheep muscle fatty acid content, to solve the technical problems that local sheep breed sample quantity is limited, traditional single gene analysis method is difficult to analyze fatty acid metabolism regulation from network level.This application carries out transcriptome sequencing to multiple months of muscle tissue of Gangba sheep, constructs gene expression matrix, using weighted gene co-expression network analysis (WGCNA) Combined with module characteristic gene and fatty acid phenotype correlation screening strategy, the pivot gene significantly positively correlated with muscle fatty acid content is obtained.The screening method can construct a robust co-expression network under limited sample size, systematically identify the functional module and core gene related to the content of fatty acids such as linoleic acid, and reveal the dynamics of fatty acid metabolism at different ages, and the method can be extended to other plateau livestock;The screened pivot gene can be used as a molecular breeding marker for early selection of Gangba sheep, and the breeding cycle is shortened.

An induction method for an Antirrhinum majus L. hairy root producing an anthocyanin and a glycoside derivative thereof, a method for screening an inducing gene, and production and use, specifically belonging to the technical field of plant tissue culturing. The induction method for an Antirrhinum majus L. hairy root producing an anthocyanin and a glycoside derivative thereof comprises the following steps: using Agrobacterium rhizogenes containing an inducing gene to infect an Antirrhinum majus L. explant, co-culturing the Agrobacterium rhizogenes and the Antirrhinum majus L. explant, and performing sterilization culture to obtain the Antirrhinum majus L. hairy root producing the anthocyanin and the glycoside derivative thereof. The inducing gene comprises a gene encoding an MYB transcription factor, or a mutant thereof. The induction method can quickly and effectively obtain the hairy root synthesizing the anthocyanin and the glycoside derivative thereof while realizing the acquisition of the anthocyanin and the glycoside derivative thereof in a short time without being affected by seasons in the case of limited germplasm resources.

The invention relates to the technical field of bioinformatics and distributed computing, and discloses a millet gene screening method and system based on data mining and a storage medium, and the method comprises the steps: obtaining millet gene data, carrying out initial clustering processing, and dividing the millet gene data into a plurality of data segments to obtain a segment set; evaluating a resource demand index of each data segment in the segment set; obtaining current load state data of the computing nodes, and distributing the data segments to the corresponding computing nodes to generate task distribution mapping; starting a gene analysis task by adopting a parallel execution mechanism, monitoring an execution progress and generating a real-time synchronous adjustment signal; carrying out migration adjustment on the task according to the adjustment signal, optimizing a task execution sequence, collecting a gene analysis result of each calculation node, and fusing key site information to obtain an integrated analysis data set; performing verification processing on the analysis data set, and outputting a final millet gene screening result; the resource utilization rate and the analysis efficiency during gene data processing are improved.

The present application relates to a method for screening for a gene. Some embodiments of the present application provide a method for screening for a cell resetting gene. Some embodiments of the present application provide a method for screening for a therapeutic gene.

The invention relates to an anti-Theras gene derived from a cold spring, a screening method, an activity determination method and application of the anti-Theras gene, and particularly discloses an anti-Theras gene derived from the cold spring, and the nucleotide sequence of the anti-Theras gene is shown as SEQ ID NO: 1. According to the application, a novel anti-Theras gene is identified from a deep sea cold spring environment for the first time, and heterologous expression and plaque experiments prove that the novel anti-Theras gene can effectively antagonize a Theras defense system of a host. According to the anti-Theras gene provided by the invention, the types and sources of anti-defense genes are expanded, and a gene blank for non-classical defense system Theras from a cold spring environment is provided.

The invention relates to an anti-defense gene gad1 from a cold spring, a screening method, an activity determination method and application thereof, and particularly discloses the anti-defense gene gad1 from the cold spring, and the nucleotide sequence of the anti-defense gene gad1 is as shown in SEQ ID NO: 1. The anti-defense gene gad1 provided by the invention is derived from the cold spring virus, the sequence and the structure of the anti-defense gene gad1 can adapt to extreme environments such as low temperature and high pressure, the anti-defense activity is exerted, and the stable activity is kept in the ecological niche.

The application discloses a rice tricyclazole-resistant gene screening method and system based on phenotype analysis, and relates to the technical field of plant gene screening; the method comprises the following steps: based on induced leaves, pre-induction leaves are subjected to induction treatment, and time-series phenotype data of the pre-induction leaves and post-induction leaves are collected to obtain induction data; based on baseline leaves, pre-baseline leaves are subjected to baseline treatment, and time-series phenotype data of the pre-baseline leaves and post-baseline leaves are collected to obtain baseline data; based on the induction data and the baseline data, disease course extraction is performed on the time-series phenotype data of the pre-baseline leaves to obtain basic resistance data; and based on material site data, post-treatment performance data, the basic resistance data and induction gain data, tricyclazole-resistant gene data is screened out; the application can distinguish the tricyclazole induction influence from the difference in the self resistance of rice materials, and realizes stable screening of tricyclazole-resistant related genes.

The invention provides a method for screening key hormones and genes for lycium ruthenicum anthocyanin synthesis. The key hormones comprise 1-aminocyclopropane carboxylic acid, salicylic acid, abscisic acid and dihydrozeatin. The key genes comprise an MYB80 gene, an MYB102 gene, a K21383 enzyme family gene, an ARR2 gene and a BHLH14 gene; the screening method comprises the following steps: collecting lycium ruthenicum and lycium barbarum samples, washing with ultrapure water, removing surface moisture, freezing and fixing with liquid nitrogen, performing freeze drying, grinding, sieving, and respectively performing transcriptome analysis and hormone group analysis. The invention further provides application of the key hormones and the genes screened by the method, and the key hormones and the genes can be used as molecular markers or genetic manipulation targets to research and regulate signal pathways for synthesizing anthocyanin of lycium ruthenicum. Key hormones and key genes participating in synthesis of lycium ruthenicum anthocyanin are analyzed and screened by using a hormone group and a transcriptome, and the synthesis of the lycium ruthenicum anthocyanin jointly regulated by dynamic transcriptional regulation and hormone interaction is disclosed.

This invention provides a high-throughput and efficient method for screening multiple target genes that can regulate mRNA expression levels in cells by co-transfecting or sequentially transfecting mRNA with a gene silencing library targeting genes in cells using lipid nanoparticles. Several target genes that can regulate mRNA protein expression levels through the action of siRNA have been discovered. By manipulating these genes that regulate mRNA expression levels, the translational capacity of mRNA in mRNA vaccine and drug development can be improved, mRNA protein expression efficiency can be increased, and immunogenicity and toxicity can be reduced.

The application relates to an endometriosis causal gene screening method and device based on cross-brain region genetic regulation integration analysis, equipment and a medium, and relates to the technical field of biomedical testing. The method constructs a cross-system genetic analysis platform, realizes a reproducible cross-tissue genetic integration analysis framework by integrating brain region eQTL data and large sample GWAS statistical results, and comprises the following steps: identifying a potential pathogenic path and a candidate causal gene of central expression factors by using eQTL data and GWAS data, covering data acquisition, tool variable construction, causal inference modeling, linkage disequilibrium control and functional annotation analysis, realizing cross-system analysis of a genetic regulation mechanism of an endometriosis pathogenic risk, and focusing on exploring a remote action path of brain region homeostatic expression on disease susceptibility.

The invention provides an induction method of hairy roots of antirhubarb for producing anthocyanin and glucoside derivatives thereof, an induction gene screening method and production application, and particularly belongs to the technical field of plant tissue culture. The induction method of the hairy roots of the antirnus auratus for producing the anthocyanin and the glycoside derivatives of the anthocyanin comprises the following steps: infecting an explant of the antirnus auratus by using agrobacterium rhizogenes containing induction genes, and carrying out co-culture and degerming culture to obtain the transgenic hairy roots for producing the anthocyanin and the glycoside derivatives of the anthocyanin, the inducer gene comprises a gene for coding an MYB transcription factor or a mutant of the MYB transcription factor. According to the induction method, the hairy roots for synthesizing the anthocyanin and the glucoside derivatives thereof can be rapidly and effectively obtained, and meanwhile, the anthocyanin and the glucoside derivatives thereof can be obtained in a short time without being affected by seasons under the condition that germplasm resources are limited.