Plant genetic transformation screening carrier and application thereof

A genetic transformation and plant technology, applied in the field of plant genetic transformation screening vectors, can solve problems such as safety risks of exogenous selection markers, and achieve commercial applications, reduce concerns about safety risks, and have good market value and social benefits. Effect
CN110408646AActive Publication Date: 2019-11-05HAINAN BOLIAN RICE GENE TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HAINAN BOLIAN RICE GENE TECH CO LTD
Publication Date
2019-11-05

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Abstract

The invention provides a plant genetic transformation screening carrier and an application thereof. The carrier uses an expression box containing a rice P450 gene(CYP81A6) as a screening label, bentazon or bensulfuron methyl is used as a screening agent for screening in the callus tissue stage, and the obtained plant is detected to be a genetically modified positive plant and has high resistance to herbicide. The plant genetic transformation carrier can be used as the genetically modified screening carrier, and through cooperation with other functional elements, in the genetically modified process, rice autogene is used, and exogenous screening label genes of bacterium sources and the like are not introduced, so that the genetically modified screening method for plants is enriched, the concern about security risks of genetically modified plants can also be reduced by public, promotion of business application of the genetically modified plants is facilitated, and the plant genetic transformation screening carrier has favorable market value and social benefits.
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Description

technical field

[0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a plant genetic transformation screening carrier and its application. Background technique

[0002] Due to its many advantages, transgenic technology can broaden the available genetic resources and create new germplasm resources; it can conduct directional and fixed-point mutation and selection of plant traits; The scope is getting wider and wider. Since GM crops were approved for commercial planting in 1996, in the past 21 years, a total of 23 GM crops have been approved for commercial production in the world, involving more than 16 diseases including insect resistance, herbicide resistance, disease resistance, fertility change, and quality improvement. Class target traits. According to statistics, in 2016, 26 countries and regions around the world planted herbicide-resistant and insect-resistant soybeans, corn, cotton, rape and other genetically modified ...

Claims

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