54 results about "Bentazon" patented technology

The invention discloses a recombinant vector and a non-GMO gene editing plant screening method. An initial carrier in the recombinant vector is a CRISPR/Cas9 carrier which contains sgRNA gene, Cas9 gene and screening label gene and is used for plant gene editing, the recombinant vector carries a BelRNAi expression element, and a hairpin RNA interference fragment interfering with bentazone resistance gene is generated through transcription of the BelRNAi expression element. By introducing the BelRNAi expression element to the initial carrier and conducting bentazone herbicide screening on future generation plants, offspring with target gene mutated are reserved, and it is also ensured that the mutated offspring do not contain transgenic fragments; screening method is cheaper, simpler and more effective.

The invention provides an acquisition method for transgenic gramineous crops capable of being selectively perished, comprising the following steps: when target genes are expressed, expression of genes participating in herbicide detoxification in the transgenic gramineous crops is inhibited by utilization of antisense RNA or RNAi means, and the transgenic gramineous crops capable of being selectively perished are obtained. The invention mainly has the advantages that: the transgenic gramineous crops acquired by utilization of the method can be selectively perished by Bentazon or sulphuryl carbamide herbicide as required, thereby transmission of the transgenic gramineous crops and interfusion to non-transgenic gramineous crops are prevented, and the method has great significance in transgenic crop research later on.

A synergistic mixture of penoxsulam and bentazon controls weeds in crops, especially rice and other cereal and grain crops, pastures, rangelands, IVM and turf. In addition to providing improved post-emergence herbicidal weed control, the mixture safens damage to rice.

The invention discloses a weeding composition for a cornfield. The weeding composition is prepared by combining nicosulfuron as an active ingredient and bentazon; and the weight ratio of the nicosulfuron to the bentazon is 1:30-40:1. The composition can be made into missible oil, oil deflocculant, wettable powder, dry deflocculant and the like according to a well-known method. The weeding composition has the characteristics of high-efficient weeding effect, wide weed spectrum, little use amount, low residue, safety to crop, cold and drought resistance, low cost and good prevention and control effect on broadleaf weed and grassy weed.

The invention provides a paddy field herbicide composition with bentazon and bispyribac-sodium as active ingredients. The herbicide composition comprises 10wt%-92wt% of bentazon and bispyribac-sodium, 0-5wt% of agriculturally acceptable adhesive, 0-5wt% of stabilizer, 0-3wt% of cosolvent, 2-5wt% of synergistic agent, 5-10wt% of surface active agent and the balance of agriculturally acceptable carrier or mixture of water and alkaline, wherein the weight ratio of the bentazon and the bispyribac-sodium is 15:1 to 50:1. The composition can be prepared into particles, wettable powders or water solutions, is used for controlling and removing grassy, broad and sedgy weeds in the transplanted paddy fields or seedling transplanting fields, has the advantages of safe use, flexibility and convenience, and also can be flung, sprayed and atomized.

The invention discloses a maize cytochrome P450 gene, which has the nucleic acid sequence showed by SEQ ID NO: 1. The transgenic crop obtained by the gene has the capability of increasing the high resistance for bentazon or sulfonylurea herbicides. The invention also discloses a DNA plasmid, which contains an inhibiting expression frame of antisense RNA or RNAi constructed by the cytochrome P450 gene or the gene fragment of the cytochrome P450 gene. The invention also discloses a transgenic maize cell and a preparation method of transgenic maize by using the cell. The transgenic crop obtained by the DNA plasmid of the invention can decrease the resistance ability for bentazon or sulfonylurea herbicides, so that the bentazon or sulfonylurea herbicides can be applied to control the transgenic crop selectively.

The invention relates to the technical field of pesticides, and especially discloses a cornfield weeding composition. Effective components of the cornfield weeding composition comprise mesotrione, pendimethalin and bentazon, and a weight ratio of mesotrione to pendimethalin to bentazon is 1-10:4-25:5-40. Mesotrione, pendimethalin and bentazon have different action mechanisms and different weed action points, and the weed control spectrums of mesotrione, pendimethalin and bentazon are complementary, so the cornfield weeding composition has the advantages of substantially enlarged weed control spectrum, substantially improved preventing and killing effects, prevention and killing of cornfield grassy weeds and broadleaf weeds, reduction of the consumption of the effective components per unit area, alleviation of the generation of the drug resistance of weeds, reduction of the pollution to environment, and reliable safety to corn and later crops.

The invention belongs to the field of chemical engineering, and particularly relates to a wastewater-free preparation method of bentazon. According to the technical scheme, isatoic anhydride is used as a starting raw material, an aprotic polar solvent is used as a reaction medium in aminolysis of isopropylamine to produce amides, mixture is subjected to sulfonation of chlorosulfonic acid and cyclization, alkaline solution is directly used for extraction, and bentazon water aqua can be obtained directly. The preparation method has the beneficial effects that the yield is high, the full yield reaches above 85 percent, no wastewater is produced in the entire process, raw materials can be obtained easily, the operating is simple, and the industrialization can be realized easily.

The invention discloses a herbicidal composition containing bentazone and cyhalofop-ethyl. The herbicidal composition uses bentazone and cyhalofop-ethyl as main active ingredients, and the mass ratio of bentazone and cyhalofop-ethyl is 1~80:1~80. The herbicidal composition of the present invention is applied to post-emergence weeding in paddy fields, especially for controlling gramineous and broad-leaved weeds in paddy fields, and has remarkable effects. Compared with a single agent, the herbicidal composition of the present invention delays weed resistance, has a wide herbicidal spectrum, long duration of action and is safe to rice and subsequent crops under the premise of improving the efficacy of a single agent.

The invention discloses a herbicidal composition containing bentazone and fenoxaprop-p-ethyl. The herbicidal composition uses bentazone and fenoxaprop-p- The mass ratio of grass spirit is 1-60:1-60. The herbicidal composition of the invention can be used for post-emergence weeding in soybean fields, especially for controlling gramineous and broad-leaved weeds in soybean fields and has remarkable effects. Compared with a single agent, the herbicidal composition of the present invention delays weed resistance, has a wide herbicidal spectrum, long duration of action, and is safe for soybeans and subsequent crops under the premise of improving drug efficacy.

The invention provides a plant genetic transformation screening carrier and an application thereof. The carrier uses an expression box containing a rice P450 gene(CYP81A6) as a screening label, bentazon or bensulfuron methyl is used as a screening agent for screening in the callus tissue stage, and the obtained plant is detected to be a genetically modified positive plant and has high resistance to herbicide. The plant genetic transformation carrier can be used as the genetically modified screening carrier, and through cooperation with other functional elements, in the genetically modified process, rice autogene is used, and exogenous screening label genes of bacterium sources and the like are not introduced, so that the genetically modified screening method for plants is enriched, the concern about security risks of genetically modified plants can also be reduced by public, promotion of business application of the genetically modified plants is facilitated, and the plant genetic transformation screening carrier has favorable market value and social benefits.

The invention discloses a herbicide for a bean field. The herbicide is characterized by comprising the following components in part by weight: 20 to 90 parts of 5 percent refined quizalofop missible oil; 20 to 150 parts of 48 percent aqueous bentazon and 5 to 30 parts of 10 percent fluoroglycofen-ethyl missible oil. A method for preparing the herbicide comprises the following steps of: adding the 5 percent refined quizalofop missible oil into the 48 percent aqueous bentazon; stirring and mixing the mixed solution; and mixing the 10 percent fluoroglycofen-ethyl missible oil with the two solutions to obtain the herbicide for the bean field. When the herbicide is sprayed in the bean field, a plurality of weeds can be killed at one time. The herbicide is safe and reliable, has a terminating rate of 99.4 percent, reduces the use amount of a single herbicide and reduces cost and labor intensity simultaneously. The herbicide for the bean field can enlarge a weed-killing spectrum, can radically kill a plurality of weeds quickly at one time, has no harmful effect on the bean field and after-cultured plants and is a harmless bean field post-emergency herbicide.

The invention discloses a synergistic weeding composition comprising clopyralid and bentazone. The weeding composition takes clopyralid and bentazone as main active ingredients in a mass ratio of 1-60:1-60. The synergistic weeding composition can be applied to the preparation of a weeding agent for weeding after seedlings are planted in a wheat field. Compared with a single agent, the weeding composition delays weed resistance, has a broad weeding spectrum, acts for a long time, is safe for wheat and afterculture crops and has particularly remarkable effect when applied to the prevention and killing of various broadleaf weeds in wheat fields on the premise of enhancing medicament effect.

The invention provides a rice CYP81A6 gene mutant CYP81A6-m2 and application thereof and belongs to the technical field of genetic engineering. Indica variety 93-11 undergoes radiation-induced mutation through cobalt-60, so that 23 base pairs in a second exon of a rice CYP81A6 gene are deleted, the CYP81A6 gene mutant is named as CYP81A6-m2, the nucleotide sequence of the CYP81A6 gene mutant is as shown in SEQ ID No.1, the high sensitivity of CYP81A6-m2 to bentazone herbicide is further verified, and the CYP81A6-m2 can be used for preparing genetically modified rice sensitive to bentazone and have significant effects in the genetic improvement and seed breeding of rice germplasm resources. The invention further provides a method for molecular marker identification of the mutant and application thereof to seed breeding and seed production.

The invention discloses a herbicidal composition containing bentazone and clodinafop-propargyl. The herbicidal composition uses bentazone and clodinafop-propargyl as main active ingredients, and the mass ratio of bentazone and clodinafop-aparyl is 1-80. : 1-80. The herbicidal composition of the present invention is applied to post-emergence weeding in wheat fields, especially for controlling various gramineous and broad-leaved weeds in wheat fields, and has remarkable effects. Compared with a single dose, the herbicidal composition of the present invention delays weed resistance, has a wide herbicidal spectrum, long duration of action and is safe to wheat and subsequent crops on the premise of improving the efficacy of a single agent.

The invention relates to the field of agricultural herbicides, particularly to weeding composition containing tembotrione and bentazone. The weeding composition comprises effective ingredients including the tembotrione and the bentazone, wherein the weight percentage of the effective ingredients is 8%-80%, and the weight ratio of the tembotrione to the bentazone is (30:1)-(1:30). Dosage forms such as aqueous suspending agents, oil suspending agents, suspension emulsions, emulsifiable concentrates, micro-emulsions, aqueous emulsions, wettable powder, water dispersible granules, granules and dry suspending agents can be prepared. The weeding composition is used for controlling weeds in corn fields or other places, has the characteristics that the control effect on the weeds is improved, the weeding spectrum is increased, the pesticide dosage is decreased and the like, meets the national environment protection requirements, and conforms to the national policy requirement for reduction of the pesticide dosage in the future.

The invention provides a rural-harmful-liana integrated control method. The rural-harmful-liana integrated control method includes the steps of chemical controlling, wherein a chemical prepared from acomposition of picloram, 2,4-dichlorophenoxyacetic acid, bentazone and sulfometuron-methyl is sprayed onto vines and stems of rural harmful liana according to the applying quantity of 3,000 ml hm<-2>to 5,000 ml hm<-2>, and the leaf withered rate of the rural harmful liana is up to 50% or above; clearing, wherein the rural harmful liana obtained after chemical controlling is cleared to remove thevines and the stems of the rural harmful liana on the ground; ecological controlling, wherein mixed planting of quick-growing species of trees is conducted in a cleared region according to the plantspacing and the line spacing of 1.0 m to 2.0 m*1.5 m to 3.0 m, the trees are cultured for 1 year to 2 years, and growth and spreading of the rural harmful liana are gradually controlled. According tothe rural-harmful-liana integrated control method, the dose of the applied chemical is low, soil pollution is small, the rural harmful liana causing large-area harm can be efficiently killed, a laterecological system of a killing region can be maintained to be stable, and the ecological safety performance is high.

The invention discloses a herbicidal composition containing bentazone and clethodim. The herbicidal composition uses bentazone and clethodim as main active ingredients, and the mass ratio of bentazone to clethodim is 1-60. : 1~60. The herbicidal composition of the present invention can be applied to post-emergence weeding in soybean fields, especially for controlling various gramineous and broad-leaved weeds in soybean fields, and has remarkable effects. Compared with a single dose, the herbicidal composition of the present invention delays weed resistance, has a wide herbicidal spectrum, long duration of action and is safe to soybeans and subsequent crops under the premise of improving the efficacy of a single agent.

The invention discloses a herbicidal composition containing bentazone and milinadin and its application. The herbicidal composition uses bentazone and milinadin as main active ingredients, wherein the mass of bentazone and milinadin The ratio is 1-80:1-80, and the herbicidal composition of the present invention can be applied to weeding after emergence in paddy fields, especially in the control of gramineous and broad-leaved weeds in paddy fields with remarkable effects. Compared with a single dose, the herbicidal composition of the present invention delays weed resistance, has a wide herbicidal spectrum, long duration of action and is safe to rice and subsequent crops under the premise of improving the efficacy of a single agent.

The invention belongs to the field of herbicides and discloses a mixed herbicide containing bentazone, acifluorfen and sethenoxydim and an application thereof. The herbicide uses bentazone, acifluorfen and sethoxydim as main active ingredients, and the mass ratio of bentazone, acifluorfen and sethoxydim is 0.1~80:0.1~80:0.1~80 , the total mass percentage of the three in the herbicide is 1-85%. The application of the mixed herbicide of the present invention in post-emergence weeding in soybean fields, especially in the application of controlling various gramineous or broad-leaved weeds in soybean fields has remarkable effects. Compared with a single dose, the herbicide of the present invention delays weed resistance, has a wide herbicide spectrum, long duration of action and is safe to soybeans and subsequent crops under the premise of improving the efficacy of a single agent.

A technique for eliminating female parent self-bred seedlings from the hybridized rice seedlings with herbicide features that the female parent with recessive character of sensitive lethality to bentazon is chosen to hybridize with recovery line and during the stage from green presentation to ear sprouting the solution of bentazon (10 mg/L-5000 mg/L) is sprayed onto their leaves to eliminate said self-bred seedings for keeping the high-purity of hybridized rice seedlings. It can be used in high-speed discrimination of purity for hybridized rice seedlings.

The invention discloses a process and a device for continuously producing bentazone intermediate o-aminobenzoic acid isopropyl amine by taking phthalimide as a raw material. The process comprises thefollowing steps: (1) adding phthalimide solid and pure water into a reaction kettle, stirring at a low speed, adding a 32% sodium hydroxide solution, and stirring at normal temperature for 30 minutes;(2) respectively feeding the phthalimide sodium salt solution and the sodium hypochlorite solution into a micro-channel reactor according to a certain flow rate, carrying out a Huffman rearrangementreaction at a low temperature, and feeding the mixture into a temporary storage tank; (4) mixing the reaction solution in the temporary storage tank 1 with hydrochloric acid (or sulfuric acid and thelike) through a static mixer, feeding the mixture into a reaction kettle, heating the mixture to room temperature, adding an isopropylamine aqueous solution at a certain flow rate, and continuously stirring for 2-3 hours; (4) enabling the solution to pass through a plate-and-frame filter press, returning the filtrate to the first step for repeated application, and drying the filter cake to obtainisatoic anhydride with the purity of 99% or above.

The invention discloses a synergistic herbicidal composition containing picloram and bentazon and application thereof. The active ingredients of the herbicidal composition are picloram and bentazon in a mass ratio of 1-70:1-80. The synergistic herbicidal composition can be used for controlling post-emergence weeds in wheat fields and particularly has an obvious effect of controlling various broadleaf weeds in the wheat fields. Compared with a single-component herbicide, the herbicidal composition delays the resistance of the weeds, has a broad herbicidal spectrum and acts for a long time on the premise of improving the herbicide effect, and is safe for wheat and afterculture crops.