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Method for rapidly identifying bentazon sensitivity rice

An identification method and technology of bentazon, applied in the field of plant identification, can solve the problems of large identification error, high cost, long identification time, etc., and achieve the effects of high accuracy, simple method and low labor cost.

Inactive Publication Date: 2011-09-07
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of high cost, long identification time and large identification errors in the identification of bendazone-sensitive rice, the method of the present invention is based on the D 1 Sensitivity method for rapid identification of rice using the inhibition rate of chlorophyll fluorescence parameter Yield (PSⅡ effective photochemical quantum yield) after bentazone treatment on leaves

Method used

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  • Method for rapidly identifying bentazon sensitivity rice
  • Method for rapidly identifying bentazon sensitivity rice
  • Method for rapidly identifying bentazon sensitivity rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Major equipment:

[0033] 1. Chlorophyll fluorescence instrument; 2. Micropipette; 3. Electronic scale.

[0034] Materials and Reagents:

[0035] 1. Materials: rice leaves of different sensitive types

[0036] 2. Main reagents:

[0037] (1) 25% bentazone herbicide: commercially available.

[0038] (2) Tween 20: commercially available.

[0039] (3) 0.02% by volume (V / V) bentazone treatment solution: take 0.8 mL of 25% bentazone herbicide, add 2.5 mL of Tween 20, and distill the volume to 1000 mL, which is 0.02% by volume (V / V) Bentazon treatment solution.

[0040] (4) Control solution: add 2.5 mL Tween 20 to 1000 mL distilled water to obtain a 0.25% volume percentage (V / V) Tween 20 control solution without bentazone.

[0041] The rapid identification method of bendazone-sensitive rice includes the following steps:

[0042] (1) Marking of rice leaves: rice is cultivated in the field and managed normally. At the 6-leaf stage, two healthy leaves were selected from t...

Embodiment 2

[0052] Major equipment:

[0053] 1. Chlorophyll fluorescence instrument; 2. Micropipette; 3. Electronic scale; 4. Hole punch.

[0054] Materials and Reagents:

[0055] 1. Materials: rice leaves of different sensitive types, stored at 4°C for later use;

[0056] 2. Main reagents:

[0057] (1) 25% bentazone herbicide: commercially available.

[0058] (2) Tween 20: commercially available.

[0059] (3) 0.02% by volume (V / V) bentazone treatment solution: take 0.8 mL of 25% bentazone herbicide, add 2.5 mL of Tween 20, and distill water to 1000 mL, which is 0.02% by volume (V / V) Bentazon treatment solution.

[0060] (4) Control solution: Add 2.5 mL Tween 20 to 1000 mL of distilled water to obtain a 0.25% (V / V) Tween 20 control solution without bentazone.

[0061] Operation method:

[0062] (1) Marking of rice leaves: Take the rice leaves in the field back to the laboratory, and after marking, place them in a petri dish covered with wet gauze to prevent the leaves from dehydrat...

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Abstract

The invention relates to a method for rapidly identifying bentazon sensitivity rice, and the method can be used for solving the problems of high cost, long identification time and great identification errors of the bentazon sensitivity rice identification method in a known technique. The method provided by the invention comprises the following three steps: marking rice leaves; coating bentazon; and rapidly identifying the sensitivity rice, wherein the suppression rate of the chlorophyll fluorescence parameters Yield (PS II effective photochemical quantum yield) is utilized to identify the sensitivity of the rice after the leaves are coated with the bentazon. The method provided by the invention has the advantages of simplicity, low manpower cost and high identification accuracy; the mutant identification can be finished after the bentazon processing is carried out for 7 hours; the false positive interference can be effectively eliminated; 0.02%(V / V) bentazon is utilized as a bentazon herbicide, and the concentration of the bentazon is obviously lower than that of the 0.1%(V / V) bentazon used in the traditional identification method; and the damage to the ecological environment is effectively reduced when a large amount of herbicide is used.

Description

technical field [0001] The invention belongs to the technical field of plant identification, and in particular relates to a rapid identification method for bendazone-sensitive rice. Background technique [0002] Bentazon is a broad-spectrum, high-efficiency, and low-toxicity benzothiadiazole (BTH) herbicide. Commonly used trade names include Basagra, Bentazone, Bazak, Thiazapine, and Patriol. Wait. The active ingredient of bendazone can be absorbed through the roots and leaves of plants, and it is effective for most dicotyledonous plants [except soybean (Glycine max Merrill L.), peanut (Arachis hypogaca L.)] and sedge (Cyperus L. ) family weeds have a killing effect and are harmless to grasses. As a selective systemic herbicide, bentazon is generally believed to target the D of photosystem II. 1 A protein that blocks the non-cyclic photosynthetic electron transport chain, inhibits photosynthesis, and causes the death of susceptible plants. [0003] The wild-type rice var...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/28
Inventor 王云生周俊峰张平泰高丽萍王景龙
Owner ANHUI AGRICULTURAL UNIVERSITY
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