Reference gene screening method for stable expression of suaeda salsa under salt stress

Abstract

The invention discloses a reference gene screening method for stable expression of suaeda salsa under salt stress. According to transcriptome analysis results obtained in a laboratory and reported reference gene sequence information of other plants, fourteen reference gene candidates with relatively stable expression are preliminarily screened and named as ACT7, ACT1, CCD1, TUA5, UPL1, UBC28, EF1alpha, PP2A, DREB1D, TIM, V-H(+)-ATPase, MPK6, PHT4:5 and CESA1 respectively. For these gene candidates, corresponding amplification primers are designed and reference gene screening is conducted. Tengenes stably expressed in a gene expression profile of suaeda salsa are screened out as the reference genes of suaeda salsa under salt stress, and the stability and reliability of suaeda salsa gene expression analysis and research under salt stress conditions are easily improved. The method makes up for the defect of an existing method in which the use of a single reference gene can lead to errorsand deviations in experimental results, a more reliable conclusion can be obtained, and the method is of great significance for promoting the improvement and perfecting of molecular biological methods and revealing cell differentiation, development and morphogenesis.

Description

technical field [0001] The invention relates to the field of plant molecular biology, in particular to a screening method and application of an internal reference gene for stable expression of Suaeda salsa under salt stress. Background technique [0002] Suaeda plants are important halophyte resources. There are more than 100 species in the world. They mainly grow in saline-alkaline wastelands such as seashores, lakesides and deserts. They are a typical indicator plant in saline-alkali land. There are 20 species and 1 variety of Suaeda genus in my country, which are widely distributed. At present, people's research on Suaeda genus mainly focuses on its cultivation and tissue culture, development and utilization of salt-tolerant genes, ion transport and signal transduction, etc. Some scholars have also studied the absorption effect of Suaeda genus plants on heavy metal elements under hydroponic conditions, and the bioremediation effect of Suaeda salsa plants on eutrophic wat...

AI Technical Summary

Problems solved by technology

A wide range of experimental data shows that the stability of internal reference genes may be affected by conditions such as different tissues, different developmental stages, or different environmental stresses, so a single internal reference may lead to biased, inaccurate or even wrong experimental results

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