38 results about "Gene Organization" patented technology

The invention discloses a cloud management system and method for gene data analysis and processing, and the method comprises the steps: firstly receiving and storing structural data transmitted from an edge calculation module in a 5G wireless transmission mode; calling the uploaded gene structured data and the original data of the fluorescence signals of the genes stored in the edge computing equipment according to the corresponding retrieval information, performing personalized display of different functions by utilizing the data, and performing analysis and mining on the gene data by utilizing an artificial intelligence related model; and finding out information with relatively high value for service requirements to carry out secondary development of corresponding functions. Besides, when there is abnormal information transmitted by the edge computing module and suspected abnormal samples obtained by analyzing the structural data by related applications in the system, an alarm is sent to the abnormal processing module in time, the abnormal processing module is reminded to take corresponding measures on the corresponding abnormal information, and the application effect of actual production is improved.

The invention relates to a method for changing the feeding habits of the bombyx mori and application of the method. The hestia gene of the bombyx mori is targeted at a fixed point, a gene structure ofthe bombyx mori is damaged to affect normal feeding behavior of the bombyx mori, and thus, the feeding habits of the bombyx mori are changed into omnivory from oligophagy. By the method, a breeding mode of feeding the bombyx mori with folium mori only in sericultural production is solved, and a new through is provided for large-scale bombyx mori feeding.

A Bacillus thuringiesis 15A3 strain is disclosed, which has unique cry gene organization including crylAa, crylAc, crylc, crylD, coyII and cryV. It is obtained by screening with PCR method and bioactivity measuring method. Its wettable powder and liquid preparation has high effect on preventing and eliminating the pests, such as beet noctuid, cotton ballworm, etc..

A duplex-seq-based ultra-low frequency mutation site detection and analysis method disclosed in the present invention comprises the following steps: 1) evaluating the quality of original sequencing data, reducing data noise, and providing effective data for subsequent analysis; 2) random barcode Extract the title line of each sequence of the sequence file, which is convenient for subsequent quick retrieval of barcode and creation of consistent sequences; 3) Create consistent sequences based on family barcode and duplex barcode, and exclude mutations introduced during the library construction process or PCR process ; 4) Construct a double-stranded consensus sequence according to duplex-tag, and further exclude asymmetric mutation sites in the sequence; 5) Perform local quality correction on the compared data, and perform low-frequency mutation site detection; Three-level annotation of gene structure, function, and clinical phenotype; 6) Statistics of SSCS, DCS sequence numbers, comparison results, and variation site information, and output visual charts.

The invention belongs to the technical field of biogenetic engineering, and in particular relates to a NAC47 transcription factor of Brassica napus and its preparation method and application. Specifically disclosed the gene structure of NAC47 transcription factor of Brassica napus, its preparation method and its application in positively regulating the senescence of rapeseed leaves; and disclosed that the expression of Brassica napus NAC47 gene can cause the necrosis of cells to cause the increase of electrical conductivity, The increase of hydrogen peroxide content, the decrease of chlorophyll content, the increase of anthocyanin content and the increase of malondialdehyde content; make the expression of RbohD, RbohF, YLS9, BFN1, CEP1 and two VPE genes better in Brassica napus controlled activation and expression.

The invention discloses a structure annotation and comparison result evaluation method of a full-length transcript. According to the disclosed comparison result evaluation and gene structure annotation method, matchAnnot software is used, an effect of a script is to modify an existing annotation gtf file and a sam file according to a format required by the matchAnnot software, the matchAnnot is used for structure annotation and comparison result evaluation, a presentation mode of a matchAnnot result is optimized, and counting is carried out.

The invention relates to an improved cotton fiber color gene technology, and particularly relates to a cotton gene and a plant expression binary vector constructed by the gene and application to improvement of cotton brown fiber color. A nucleotide sequence of the cotton GhMATE1 gene is shown in SEQ ID: NO.1. An amino acid sequence of a protein coded by the gene is shown in SEQ ID: NO.2. On the basis of an early-cloned cotton GhTT12a gene (Chinese patent application number: 201310380270.5, and data of application: 2013-8-27), by using a bioinformatics analysis and electronic cloning method, a new gene containing an MATE (multidrug and toxic compound extrusion) conserved domain database is cloned, but a gene structure and a shearing mode of the cotton GhMATE1 gene are completely different from those of the GhTT12a gene, and the cotton GhMATE1 gene has 78 percent of sequence homology with a nucleotide sequence of an arabidopsis thaliana testa brown pigment synthetic TT12 (Transparent Testa) gene.

The invention relates to a DREB transcription factor-BpDREB1 (EF219470). The overall length of a gene sequence is 647bp. A coding protein is predicted to contain 213 amino acids, the relative molecular weight of the protein is 23kDa and the theoretical isoelectric point of the protein is 5.11. The sequence homology of BpDREB1 and the transcription factors in the Chinese cabbage is 94%. An evolutionary tree shows that BpDREB1 belongs to the A1 subtribe in the DREB subfamily. The inducible expression mode analysis of the gene shows that BpDREB1 is subjected to intense and rapid inducible expression at low temperature, has response to drought stress to a certain extent but hardly has response to high salinity treatment. After transgenic arabidopsis thaliana over-expressing BpDREB1 is inducedat low temperature, the contents of soluble sugar and proline in arabidopsis thaliana are substantially increased. The above results show that the transcription factor BpDREB1 has the characteristicsof family member gene structures and plays an important role in a low-temperature and drought response pathway.

The invention discloses the application of a citrus CitPITP1 gene and its nucleotide sequence in prokaryotic vector promoter transformation. The nucleotide sequence of the CitPITP1 gene is shown in SEQ ID NO:1. The present invention screens the CitPITP1 gene from the genome of citrus, and finds from the CitPITP1 gene that a 64bp nucleotide sequence on exon 4 encoding the CRAL_TRIO domain of the CitPITP1 gene can drive gene expression in prokaryotes; therefore, the sequence containing CitPITP1_key64 The method can be applied to the transformation of a vector promoter in the field of genetic engineering.

The invention relates to a method for changing the feeding property of silkworm and its application. The invention destroys the gene structure of the silkworm hestia gene by fixed-point targeting, affects its normal feeding behavior, and promotes the feeding habit of the silkworm to change from oligophagous to omnivorous. This invention solves the breeding mode of sericulture production relying solely on mulberry leaves, and provides a new way of thinking for large-scale silkworm breeding.

The invention provides the glucose dehydrogenase GLD gene of H. bancroft and its amplification primer combination and cloning method, belonging to the technical fields of molecular biology and gene cloning. The nucleotide sequence of the GLD gene is shown in SEQ ID No.6. The acquisition of this gene can further study the composition and gene structure of the gene in the cells of Leaperia bancrofti, and provide a theory for its function and functional region location in the reproductive physiology of Leaperia banecroft, as well as the difference from other species in accordance with.

The invention provides a structural variation detection model and a construction method and device thereof. The construction method comprises the following steps: performing gene structure variation detection on sequencing data of a plurality of positive samples to obtain variation detection results; screening out characteristics of gene structure variation from variation detection results; and constructing a machine learning model by utilizing the characteristics of the gene structural variation to obtain a structural variation detection model. A reliable gene structure variation event is obtained by detecting a structure variation positive sample, then possible related characteristics of gene structure variation are screened out, and a structure variation detection model is constructed through machine learning by utilizing the possible related characteristics. Therefore, the constructed model can be used for quantitatively detecting the variation result of the to-be-detected sample more accurately. By utilizing the model, the abundance of gene structure variation events of a known sample can be corrected, and an important research direction and clinical guidance significance areprovided for more accurate quantitative detection of structure variation.

The invention provides a primer set and kit for detecting an alpha globin gene triplet, and an application of the primer set and kit. The primer set includes a primer pair for detecting alpha alpha alpha <anti3.7> and / or a primer pair for detecting alpha alpha alpha <anti 4.2>; an amplification product of the primer pair for detecting alpha alpha alpha <anti3.7> is a recombinant fragment of an alpha gene Z homeobox; and an amplification product of the primer pair for detecting alpha alpha alpha <anti 4.2> is a recombinant fragment of an alpha gene X homeobox. Based on a formation mechanism ofthe alpha globin gene triplet, and a genetic structure of alpha alpha alpha <anti3.7> and alpha alpha alpha <anti4.2>, specific primers are designed, and a multiple PCR amplification system is optimized, so that the detection of the alpha globin gene triplet alpha alpha alpha <anti3.7> and alpha alpha alpha <anti4.2> in one reaction system is realized.