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Gene sequence of Chinese cabbage dehydration transcription factor BpDREB1 and application thereof

A transcription factor and gene sequence technology, applied in the field of agricultural biogenetic engineering, can solve problems such as dehydration of Chinese cabbage, freezing damage of Chinese cabbage, and affecting the quality of Chinese cabbage

Inactive Publication Date: 2012-09-19
TIANJIN NORMAL UNIVERSITY
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Problems solved by technology

[0005] Chinese cabbage is an important vegetable variety planted in autumn in China. The sudden low temperature in autumn will cause freezing damage to Chinese cabbage, resulting in strong dehydration of Chinese cabbage, reduced yield, and seriously affecting the quality of Chinese cabbage.

Method used

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  • Gene sequence of Chinese cabbage dehydration transcription factor BpDREB1 and application thereof
  • Gene sequence of Chinese cabbage dehydration transcription factor BpDREB1 and application thereof
  • Gene sequence of Chinese cabbage dehydration transcription factor BpDREB1 and application thereof

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Embodiment 1

[0045] 1. Materials and methods

[0046] 1.1 Plant material cultivation and stress treatment

[0047] The seeds of Chinese cabbage Qiulu 60 (Chinese cabbage Qiulv60) were provided by Tianjin Vegetable Research Institute. After the seeds were sterilized, they were placed in a cultivation room with a growth temperature of 23°C, 16 hours of light, 8 hours of darkness, and 7 days of culture in MS medium. The Chinese cabbage seedlings were divided into 4 groups. The first group: without any treatment, as a control; the second group: The whole plant material was placed in a 4°C refrigerator for low temperature treatment; the third group: the culture solution was added with PEG6000 at a concentration of 10% for drought treatment; the fourth group: the culture solution was added with 250mM NaCl for high-salt treatment. The processing time is 2h, 4h, 8h, 12h, 24h, 48h in turn. The total RNA of the above materials was extracted respectively, and stored at -80°C.

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Abstract

The invention relates to a DREB transcription factor-BpDREB1 (EF219470). The overall length of a gene sequence is 647bp. A coding protein is predicted to contain 213 amino acids, the relative molecular weight of the protein is 23kDa and the theoretical isoelectric point of the protein is 5.11. The sequence homology of BpDREB1 and the transcription factors in the Chinese cabbage is 94%. An evolutionary tree shows that BpDREB1 belongs to the A1 subtribe in the DREB subfamily. The inducible expression mode analysis of the gene shows that BpDREB1 is subjected to intense and rapid inducible expression at low temperature, has response to drought stress to a certain extent but hardly has response to high salinity treatment. After transgenic arabidopsis thaliana over-expressing BpDREB1 is inducedat low temperature, the contents of soluble sugar and proline in arabidopsis thaliana are substantially increased. The above results show that the transcription factor BpDREB1 has the characteristicsof family member gene structures and plays an important role in a low-temperature and drought response pathway.

Description

[0001] This invention was funded by the key project of Tianjin Municipal Science and Technology Commission, number: 023804111. technical field [0002] The invention belongs to the technical field of agricultural biological genetic engineering, and relates to the application of a gene sequence of a cabbage dehydration transcription factor BpDREB1 in preparing transgenic frost-resistant plants. Background technique [0003] Abiotic stress is an important environmental factor affecting plant growth and development as well as crop yield and quality. During the long-term evolution process, plants have formed a variety of adaptive mechanisms to survive in adverse environments. Under adversity stress, plants adapt to the stress through a series of physiological, biochemical and molecular changes. So far, 299 drought-induced expression genes, 213 high-salt-induced expression genes, and 544 low-temperature-induced expression genes have been cloned in Arabidopsis. These genes includ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415A01H5/00
Inventor 王振英彭永康刘晓颖陈丽媛张竞秋
Owner TIANJIN NORMAL UNIVERSITY
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