Molecule marker of red peel gene of weedy rice
A molecular marker and weedy rice technology, applied in the field of molecular genetics, can solve the problems of strong grain shattering, aggravating the prevalence and flooding of weedy rice, and rice production hazards, and achieve the effect of accurate identification and differentiation, and convenient identification
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Embodiment 1
[0024] Embodiment 1, the acquisition of a molecular marker for identifying weedy rice
[0025] 1 test material
[0026] Using the weedy rice W16 collected from Yangzhong, Jiangsu, the japonica wide-compatibility variety 02428, and its hybrid F 1 , F 2 group.
[0027] 2 peel color identification
[0028] Randomly take 10 mature seeds of the material, use a rougher to remove the seed shell or directly peel off the shell by hand, and check the color of the seed peel.
[0029] 3 Red Peel Genetic Analysis Populations
[0030] In 2005, the weedy rice with red peel was collected from Yangzhong, numbered W16, and the reciprocal hybrid F 1 , planted F in 2006 1 and harvest F 2 Seed, planted F in 2007 2 Populate 200 plants, and take the leaves at the tillering stage to extract DNA, and harvest F 3 seed. When W16 is used as the female parent, the hybrid F 1 The color of the seed peel is red; when 02428 is used as the female parent, the hybrid F 1 Seed peel color is white, F ...
Embodiment 2
[0052] Example 2: Marker genotype analysis of weedy rice and cultivar resources in the Yangtze and Huaihe River basins using RID14 marker
[0053] The weedy rice materials include 3 copies of Lianyungang Ludao, 1 copy each of Huaiyuantang Rice, Lai'antang Rice, Quanjiaotang Rice and Feidongtang Rice in Anhui Province, which are preserved by the Variety Resource Room of the Institute of Grain and Crop Research, Jiangsu Academy of Agricultural Sciences, and this In recent years, the research team has collected 17 materials of weedy rice in Yangzhou, Nantong, Zhenjiang, Taizhou, and Yancheng, Jiangsu.
[0054] Other materials used for analysis include Asian cultivated rice (Oryza Sativa L.) (indica, japonica) and common wild rice (Oryza Rufipogen), the ancestor of cultivated rice; geographical distribution includes China, India, Korea, the United States, Thailand There are a total of 340 species resources. These materials include not only cultivated varieties popularized in a la...
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