6 results about "Intact cell" patented technology

This invention relates to a biomimetic nanodelivery system ANG-2-CMLNPs. The preparation method of ANG-2-CMLNPs includes the following steps: Step 1: Prepare EZ NPs using a one-step self-assembly method, then co-incubate them with siRNA to obtain siRNA-EZ NPs; Step 2: Prepare liposomes using a thin-film hydration method, then modify them with ANG-2; Step 3: Add U87 MG cells to cold PBS, centrifuge to remove intact cells and debris, and the resulting precipitate is the glioma cell membrane fusion membrane; Step 4: Mix the Angiopep-2 modified liposomes obtained in Step 2 with the glioma cell membrane fusion membrane obtained in Step 3, and sonicate to obtain a composite; Step 5: Co-extrude the composite obtained in Step 4 with the siRNA-EZ NPs obtained in Step 1 to obtain the biomimetic nanodelivery system ANG-2-CMLNPs; In the above preparation process, when EZ... When the mass ratio of NPs to siRNA is 125:1 and the mass ratio of ANG-2 modified liposomes to CM is 1:5, the prepared ANG-2-CMLNPs delivery system achieves the optimal functional balance between the ability to cross the blood-brain barrier (BBB) ​​and tumor targeting efficacy.

Compositions and methods for analysis of protein expression at the single cell level are described. The single cell proteomic technology described herein implements peptide barcode molecules to uniquely label all of the proteins and / or polypeptides present in a single cell, whereby all of the proteins / polypeptides (proteome) present in a single intact cell are uniquely labeled with a peptide barcode molecule in a partition. Polypeptides from a single cell can be uniquely barcoded and via protein sequencing, such as nanopore sequencing. Proteomic analysis of the protein sequencing data elucidates the entire proteome that was expressed in the single cell.

The invention discloses a tissue cell nucleus extraction method and a cell nucleus protective agent applied to flow sorting, and relates to the technical field of cell extraction.The tissue cell nucleus extraction method comprises the steps that operation instruments needed by an experiment are prepared and pre-cooled; cutting a fresh tissue sample into tissue blocks, and immediately transferring the tissue blocks into a precooled lysis buffer solution; manually grinding the lysis buffer solution containing the tissue block by using a homogenizer, and sampling and observing homogenate in the grinding process; filtering the homogenate product through a cell filter screen in sequence; performing low-speed centrifugation on the filtrate by adopting a centrifugal machine, and discarding supernatant to obtain primary cell nucleus precipitate; re-suspending the primary cell nucleus precipitate by using a cell nucleus washing buffer solution to obtain a purified cell nucleus precipitate; and precipitating and resuspending the purified cell nucleuses, counting by trypan blue dyeing, and calculating the percentage of the complete cell nucleuses. According to the method, the problem of more cell nucleus breakage in the tissue cell nucleus extraction process is solved through a mild homogenization strategy monitored under the microscope.

The present invention relates to the field of biotechnology and a method for creating a cDNA library. More specifically, the invention refers to a method of forming complementary DNA (cDNA) sequencing libraries from RNA in situ comprising the steps of: (a) fixating cells, immobilized on a solid surface; (b) performing an in situ reverse transcription (RT) inside cells, using RT primers comprising a PCR handle 1, and partially biotinylated dNTPs; (c) releasing single stranded cDNA from the cells using a release mix, wherein the release mix comprises an RNAse, such that the single stranded cDNA is released from intact cells; (d) collecting a supernatant comprising released cDNA into a single larger volume or in separate volumes; and (e) introducing an adapter molecule comprising a PCR handle 2 that will bind 3′ of the extended cDNA.

The invention discloses an active edible and medicinal fungus capable of being directly eaten and a preparation method thereof, and belongs to the technical field of edible and medicinal fungus processing. The method comprises the following steps: S1, preparing a solid culture medium, namely selecting one or more edible raw materials, pretreating, and adding water to prepare the solid culture medium with the water content of 50-80%; s2, sterilization treatment, wherein the solid culture medium is subjected to high-temperature and high-pressure sterilization or normal-pressure intermittent sterilization; s3, inoculation and solid culture: inoculating edible and medicinal fungus strains accounting for 3-10% of the mass of the culture medium under a sterile condition. The final product is a complex composed of active mycelia and a culture medium, an edible part comprises the culture medium degraded and converted by the mycelia and also comprises the mycelia which are spread in the culture medium, complete in cell structure and active in metabolism, and direct eating in an active form is achieved. The invention breaks the technical normal form of'processing must be inactivated 'followed for a long time in the industry, and provides a new way for instant application of edible and medicinal fungi.

The application discloses a method for modifying cell wall material of celery to improve the adsorption capacity of the cell wall material to polyphenols. The method applies ultrasonic waves to the modification process of the cell wall material of the celery, and is used for adsorption and desorption of polyphenol substances. After different pectin components are removed, the adsorption capacity of the cell wall material of the celery is changed. The cell wall material of the celery after the pectin components are removed can effectively improve the adsorption capacity and desorption capacity of the polyphenols, and improve the purity of the polyphenols. Compared with the intact cell wall of the celery without the pectin components removed, the cell wall of the celery after the different pectin components are removed has improved adsorption capacity and desorption capacity to the polyphenols. In addition, after the intact cell wall of the celery and the cell wall of the celery after the pectin components are removed are adsorbed / desorbed, the purity of the polyphenols can be improved by about 5.4 times.