1 results about "Intact cell" patented technology

This invention relates to a biomimetic nanodelivery system ANG-2-CMLNPs. The preparation method of ANG-2-CMLNPs includes the following steps: Step 1: Prepare EZ NPs using a one-step self-assembly method, then co-incubate them with siRNA to obtain siRNA-EZ NPs; Step 2: Prepare liposomes using a thin-film hydration method, then modify them with ANG-2; Step 3: Add U87 MG cells to cold PBS, centrifuge to remove intact cells and debris, and the resulting precipitate is the glioma cell membrane fusion membrane; Step 4: Mix the Angiopep-2 modified liposomes obtained in Step 2 with the glioma cell membrane fusion membrane obtained in Step 3, and sonicate to obtain a composite; Step 5: Co-extrude the composite obtained in Step 4 with the siRNA-EZ NPs obtained in Step 1 to obtain the biomimetic nanodelivery system ANG-2-CMLNPs; In the above preparation process, when EZ... When the mass ratio of NPs to siRNA is 125:1 and the mass ratio of ANG-2 modified liposomes to CM is 1:5, the prepared ANG-2-CMLNPs delivery system achieves the optimal functional balance between the ability to cross the blood-brain barrier (BBB) ​​and tumor targeting efficacy.