25 results about "Intact cell" patented technology

This invention relates to a biomimetic nanodelivery system ANG-2-CMLNPs. The preparation method of ANG-2-CMLNPs includes the following steps: Step 1: Prepare EZ NPs using a one-step self-assembly method, then co-incubate them with siRNA to obtain siRNA-EZ NPs; Step 2: Prepare liposomes using a thin-film hydration method, then modify them with ANG-2; Step 3: Add U87 MG cells to cold PBS, centrifuge to remove intact cells and debris, and the resulting precipitate is the glioma cell membrane fusion membrane; Step 4: Mix the Angiopep-2 modified liposomes obtained in Step 2 with the glioma cell membrane fusion membrane obtained in Step 3, and sonicate to obtain a composite; Step 5: Co-extrude the composite obtained in Step 4 with the siRNA-EZ NPs obtained in Step 1 to obtain the biomimetic nanodelivery system ANG-2-CMLNPs; In the above preparation process, when EZ... When the mass ratio of NPs to siRNA is 125:1 and the mass ratio of ANG-2 modified liposomes to CM is 1:5, the prepared ANG-2-CMLNPs delivery system achieves the optimal functional balance between the ability to cross the blood-brain barrier (BBB) ​​and tumor targeting efficacy.

Provided herein are methods of isolating edible biomass from a suspension cell culture comprising metazoan cells, methods of isolating intact cells from a cell culture, cell-based food products comprising edible biomass from suspension cell cultures isolated according to the methods comprising isoelectric precipitation as described herein.

The invention relates to a method of enriching a cell population containing intact and damaged cells in a liquid medium, comprising the following steps: a.) providing the liquid medium containing the cell population, b.) sedimenting the cell population out of the liquid medium, c.) separating the liquid medium and the sedimented cell population, d.) resuspending the sedimented cell population in a suspension solution to produce a cell suspension, e.) admixing the cell suspension with a ferromagnetic material that binds specifically to the damaged cells, f.) incubating the cell suspension admixed with the ferromagnetic material, g.) introducing the cell suspension into a magnetic field in order to separate the bound cells and the unbound cells in the cell suspension, and h.) separating a fraction of the cell suspension enriched with unbound intact cells, to the use of intact sperm cells enriched in the method, and to an apparatus for separating cells bound to magnetic particles in a cell suspension.

The present disclosure is directed to a non-enzymatic dissociation method which facilitates the dissociation of one or more formalin-fixed paraffin-embedded tissue samples into dissociated single cells. The present disclosure is also directed to methods of single cell analysis, i.e., methods of analyzing and / or measuring target components (e.g., biomolecules such as, but not limited to, polypeptides, polynucleotides, small molecules, and the like) on or in cells non-enzymatically dissociated from one or more FFPE tissue samples.

Disclosed are methods for isolating an analyte from a sample. In some aspects, the methods are for selectively isolating a microbial cell analyte, such as a nucleic acid, from a sample containing or suspected of containing mammalian cells. The selective isolation method includes selective lysis of the mammalian cells and filtration of the resulting lysate through a filter that retains intact microbial cells, followed by on-filter lysis of the retained microbial cells to release the microbial cell analyte. The released analyte is then eluted from the filter. In other aspects, the methods utilize on-filter lysis of a sample containing intact cells (e.g., microbial cells) to release the analytes, followed by elution of the released analytes from the filter by passing an immiscible liquid through the filter. The isolated analytes may be analyzed using a suitable assay depending on the type of analyte molecule. Also disclosed are fluidic systems and lysis solutions that may be used for isolating an analyte according to the disclosed methods.

Methods for isolating analytes from a sample are disclosed. In some embodiments, the methods are for selectively isolating microbial cell analytes, such as nucleic acids, from a sample containing or suspected of containing mammalian cells. The selective isolation method involves selective lysis of mammalian cells and filtration of the resulting lysate through a filter that retains intact microbial cells, followed by on-filter lysis of the retained microbial cells to release the microbial cell analytes. The released analytes are then eluted from the filter. In other embodiments, the methods utilize on-filter lysis of a sample containing intact cells (e.g., microbial cells) to release the analytes, followed by elution of the released analytes from the filter by passing an immiscible liquid through the filter. The isolated analytes can be analyzed using an appropriate assay depending on the type of analyte molecule.

Compositions and methods for analysis of protein expression at the single cell level are described. The single cell proteomic technology described herein implements peptide barcode molecules to uniquely label all of the proteins and / or polypeptides present in a single cell, whereby all of the proteins / polypeptides (proteome) present in a single intact cell are uniquely labeled with a peptide barcode molecule in a partition. Polypeptides from a single cell can be uniquely barcoded and via protein sequencing, such as nanopore sequencing. Proteomic analysis of the protein sequencing data elucidates the entire proteome that was expressed in the single cell.

The invention discloses a cell fusion degree auxiliary verification method and apparatus, and a server. Stem cell microscope images are collected by using three set resolution scales of an initial resolution, a down-sampling resolution and an up-sampling resolution to form a corresponding standard image group, a low-resolution image group and a high-resolution image group; and performing different image processing on different resolutions, performing cross-scale feature alignment and fusion, unifying the resolution of each scale segmentation mask, calculating a cross-scale similarity coefficient to identify a divergence region, and generating a fused segmentation result containing a cytoplasm mask, a cell nucleus mask and a complete cell region. Therefore, through multi-resolution scale image acquisition and differentiation processing, in combination with cross-scale feature alignment, dynamic weight fusion and bifurcation region identification, the problems that a single-resolution image is difficult to consider global and details in existing cell fusion degree analysis, a traditional segmentation method is insufficient in precision, and multi-scale feature fusion is low in reliability are effectively solved.

Disclosed are methods for isolating an analyte from a sample. In some aspects, the methods are for selectively isolating a microbial cell analyte, such as a nucleic acid, from a sample containing or suspected of containing mammalian cells. The selective isolation method includes selective lysis of the mammalian cells and filtration of the resulting lysate through a filter that retains intact microbial cells, followed by on-filter lysis of the retained microbial cells to release the microbial cell analyte. The released analyte is then eluted from the filter. In other aspects, the methods utilize on-filter lysis of a sample containing intact cells (e.g., microbial cells) to release the analytes, followed by elution of the released analytes from the filter by passing an immiscible liquid through the filter. The isolated analytes may be analyzed using a suitable assay depending on the type of analyte molecule. Also disclosed are fluidic systems and lysis solutions that may be used for isolating an analyte according to the disclosed methods.

The invention discloses a tissue cell nucleus extraction method and a cell nucleus protective agent applied to flow sorting, and relates to the technical field of cell extraction.The tissue cell nucleus extraction method comprises the steps that operation instruments needed by an experiment are prepared and pre-cooled; cutting a fresh tissue sample into tissue blocks, and immediately transferring the tissue blocks into a precooled lysis buffer solution; manually grinding the lysis buffer solution containing the tissue block by using a homogenizer, and sampling and observing homogenate in the grinding process; filtering the homogenate product through a cell filter screen in sequence; performing low-speed centrifugation on the filtrate by adopting a centrifugal machine, and discarding supernatant to obtain primary cell nucleus precipitate; re-suspending the primary cell nucleus precipitate by using a cell nucleus washing buffer solution to obtain a purified cell nucleus precipitate; and precipitating and resuspending the purified cell nucleuses, counting by trypan blue dyeing, and calculating the percentage of the complete cell nucleuses. According to the method, the problem of more cell nucleus breakage in the tissue cell nucleus extraction process is solved through a mild homogenization strategy monitored under the microscope.

The invention relates to a method for enriching a cell population containing intact and damaged cells in a liquid medium, comprising the steps of: a) providing the liquid medium containing the cell population, b) sedimenting the cell population from the liquid medium, c) separating the liquid medium and the sedimented cell population, d) resuspending the sedimented cell population in a suspension solution to produce a cell suspension, e) adding a ferromagnetic material to the cell suspension that binds specifically to the damaged cells, f) incubating the cell suspension containing the ferromagnetic material, g) placing the cell suspension in a magnetic field to separate the bound and unbound cells in the cell suspension, and h) separating a portion of the cell suspension enriched with unbound, intact cells.the use of intact sperm cells enriched in the process and a device for separating cells bound to magnetic particles in a cell suspension.

The present invention relates to the field of biotechnology and a method for creating a cDNA library. More specifically, the invention refers to a method of forming complementary DNA (cDNA) sequencing libraries from RNA in situ comprising the steps of: (a) fixating cells, immobilized on a solid surface; (b) performing an in situ reverse transcription (RT) inside cells, using RT primers comprising a PCR handle 1, and partially biotinylated dNTPs; (c) releasing single stranded cDNA from the cells using a release mix, wherein the release mix comprises an RNAse, such that the single stranded cDNA is released from intact cells; (d) collecting a supernatant comprising released cDNA into a single larger volume or in separate volumes; and (e) introducing an adapter molecule comprising a PCR handle 2 that will bind 3′ of the extended cDNA.

The invention belongs to the technical field of biological medicine and clinical medicine, and particularly relates to an efficient purification method of brain tissue small extracellular vesicles. The purification method comprises the following steps: cutting mouse brain tissues into uniform small blocks by using a steel wire mesh, digesting and dissociating, filtering, taking filtrate, and fixing the volume of the filtrate by using an EBSS buffer solution to obtain a tissue supernatant; the method comprises the following steps: sequentially removing complete cells, cell debris, plasma membranes and large vesicles through gradient centrifugation, filtering, taking filtrate, carrying out ultracentrifugation to obtain sEVs precipitates, and resuspending the sEVs precipitates with a PBS buffer solution to obtain a sEVs crude purification product; and preparing a single sucrose pad from a sucrose solution with the concentration of 25-35% and a PBS buffer solution, and carrying out ultracentrifugation on the sEVs crude purification product, wherein the obtained precipitate is the purified brain tissue sEVs. The purification method provided by the invention solves the problems of tedious flow, long consumed time, low yield, poor purity and the like in the existing sEVs purification technology.

A surgical treatment apparatus comprises a waterjet configured to fragment tissue and provide intact cells such as stem cells with the fragmented tissue. The intact cells can be used in one or more of many ways such as for genetic or other testing, and the intact cells can be identified as stem cells. In many embodiments, the intact cells comprise stem cells. In many embodiments, a waterjet is configured to fragment tissue. The fragmented tissue can be collected with a filter having pores sized smaller than the tissue fragments. In many embodiments cavitation with a waterjet is used to fragment the tissue comprising the intact stem cells. The waterjet may comprise a waterjet immersed in a liquid comprising water so as to form a plurality of shedding pulses. The plurality of shedding pulses can be generated with a frequency sufficient to fragment the tissue. The shedding pulses can generate cavitations that fragment the tissue.

The invention discloses a single cell printing equipment control method and system based on convolutional neural network optimization, and relates to the technical field of intelligent control, and the control method comprises the steps: continuously collecting cell suspension images containing a target single cell before jet printing operation is carried out on a cell suspension, so as to determine each frame of cell main region image, after inputting the shallow convolutional network, respectively constructing a pixel gray parameter set, a pixel response parameter set and a pixel mutation parameter set; analyzing the morphological fluctuation degree of each frame of cell main region image to screen out a standard morphological feature image; the integrity degree of a target single cell structure contained in the standard morphological feature image is analyzed, a cell integrity index is determined to judge whether the integrity degree of the target single cell structure in the current standard morphological feature image meets the printing requirement or not, and a printing control instruction of a corresponding grade is sent out and executed; accurate identification of complete cells in the cell suspension is achieved, and the printing yield and stability are improved.

The utility model relates to a detachable and combinable cell membrane flow inlay dynamic model, which comprises a plurality of arc-shaped substrates which are detachably connected end to end to form an annular structure; the first aluminum strips are arranged on the arc-shaped base plates in the circumferential direction of the arc-shaped base plates at equal intervals and are close to the inner side edges of the arc-shaped base plates; and the second aluminum strips are arranged on the arc-shaped substrates at equal intervals in the circumferential direction of the arc-shaped substrates and are close to the outer side edges. According to the detachable and combinable cell membrane flow inlay dynamic model designed by the utility model, through the plurality of detachable and combinable arc-shaped substrates, components of a cell membrane can be intuitively and three-dimensionally displayed, and each arc-shaped substrate unit can be independently assembled, so that group teaching and student practice operation are facilitated; meanwhile, all the arc-shaped substrate units can be quickly and conveniently spliced and combined into a complete cell membrane model of an annular structure, overall display can be conveniently carried out on magnetic surfaces such as a blackboard in a classroom, and the overall structure of the cell membrane is visually presented.

The application discloses a single-cell printing equipment control method and system based on convolutional neural network optimization, and relates to the technical field of intelligent control. The control method comprises the following steps: before performing a jet printing operation on a cell suspension, continuously collecting cell suspension images containing target single cells to determine cell main area images, and after inputting a shallow convolutional network, respectively constructing a pixel gray parameter set, a pixel response parameter set and a pixel mutation parameter set; analyzing the morphological fluctuation degree of each cell main area image to screen out a standard morphological feature image; analyzing the structural integrity degree of the target single cells contained in the standard morphological feature image to determine a cell integrity index, so as to judge whether the structural integrity degree of the target single cells in the current standard morphological feature image meets the printing requirements, and to issue corresponding level printing control instructions and perform the printing control instructions. The application realizes accurate identification of complete cells in the cell suspension, and improves the yield rate and stability of printing.

The present disclosure is directed to a non-enzymatic dissociation method which facilitates the dissociation of one or more formalin-fixed paraffin-embedded tissue samples into dissociated single cells. The present disclosure is also directed to methods of single cell analysis, i.e., methods of analyzing and / or measuring target components (e.g., biomolecules such as, but not limited to, polypeptides, polynucleotides, small molecules, and the like) on or in cells non-enzymatically dissociated from one or more FFPE tissue samples.

The present invention relates to the technical field of cell membrane permeability detection, and specifically to a method for quantitative detection of cell membrane permeability Δ conductivity. By studying the cell membrane permeability of the first cotyledon and the second cotyledon in the two-leaf-one-core stage of wheat, it was found that for cells with intact cell membranes, the amount of electrolyte transport across the membrane is linear within a specific time period. By detecting and calculating the changes in the conductivity of the exudate during this period through various parameters, the cell membrane permeability can be accurately reflected, and the repeatability and reliability of the results can meet the standards of quantitative research, that is, quantitative detection of cell membrane permeability is achieved; it is verified and summarized that the Δ conductivity method is used to detect cell membrane permeability, which is particularly suitable for qualitative and quantitative detection of plant cell membrane permeability. At the same time, the Δ conductivity method has application prospects in the quantitative detection of cell membrane permeability in large fungi and bacterial microorganisms. The present application provides a special conductivity detector to achieve automatic detection, avoid manual operation errors, and have high detection efficiency.

Disclosed are methods for isolating an analyte from a sample. In some aspects, the methods are for selectively isolating a microbial cell analyte, such as a nucleic acid, from a sample containing or suspected of containing mammalian cells. The selective isolation method includes selective lysis of the mammalian cells and filtration of the resulting lysate through a filter that retains intact microbial cells, followed by on-filter lysis of the retained microbial cells to release the microbial cell analyte. The released analyte is then eluted from the filter. In other aspects, the methods utilize on-filter lysis of a sample containing intact cells (e.g., microbial cells) to release the analytes, followed by elution of the released analytes from the filter by passing an immiscible liquid through the filter. The isolated analytes may be analyzed using a suitable assay depending on the type of analyte molecule. Also disclosed are fluidic systems and lysis solutions that may be used for isolating an analyte according to the disclosed methods.

The invention discloses an active edible and medicinal fungus capable of being directly eaten and a preparation method thereof, and belongs to the technical field of edible and medicinal fungus processing. The method comprises the following steps: S1, preparing a solid culture medium, namely selecting one or more edible raw materials, pretreating, and adding water to prepare the solid culture medium with the water content of 50-80%; s2, sterilization treatment, wherein the solid culture medium is subjected to high-temperature and high-pressure sterilization or normal-pressure intermittent sterilization; s3, inoculation and solid culture: inoculating edible and medicinal fungus strains accounting for 3-10% of the mass of the culture medium under a sterile condition. The final product is a complex composed of active mycelia and a culture medium, an edible part comprises the culture medium degraded and converted by the mycelia and also comprises the mycelia which are spread in the culture medium, complete in cell structure and active in metabolism, and direct eating in an active form is achieved. The invention breaks the technical normal form of'processing must be inactivated 'followed for a long time in the industry, and provides a new way for instant application of edible and medicinal fungi.

The application discloses a cassava cell wall delivery carrier and a preparation method thereof, and belongs to the field of special diet ingredient development. The application provides a preparation method of a natural cassava cell wall delivery carrier. The method is characterized in that: the whole cassava cell is separated by using pectinase, the cell wall permeability is regulated by hydrothermal treatment, the amylase is ensured to pass through the cell wall, the damage to the cell wall is minimized, the intracellular starch is enzymatically hydrolyzed by using digestive enzyme, and then a hollow cell wall with an intact cell wall structure is obtained. The obtained product can load guest molecules with biological activity, enhance the stability of the guest molecules, and slow down the release of the guest molecules in the stomach and intestines, so that the bioavailability of the guest molecules is improved.

A composition comprising cell-derived particles presenting heterologous CD24, wherein the cell is a non-cancerous cell and wherein the composition is substantially devoid of intact cells is disclosed. Methods of producing the cell-derived particles and methods of using the cell-derived particles in treatment of cytokine storm syndrome, tissue injury associated with the inflammation and Coronavirus infection are also disclosed.

The application discloses a method for modifying cell wall material of celery to improve the adsorption capacity of the cell wall material to polyphenols. The method applies ultrasonic waves to the modification process of the cell wall material of the celery, and is used for adsorption and desorption of polyphenol substances. After different pectin components are removed, the adsorption capacity of the cell wall material of the celery is changed. The cell wall material of the celery after the pectin components are removed can effectively improve the adsorption capacity and desorption capacity of the polyphenols, and improve the purity of the polyphenols. Compared with the intact cell wall of the celery without the pectin components removed, the cell wall of the celery after the different pectin components are removed has improved adsorption capacity and desorption capacity to the polyphenols. In addition, after the intact cell wall of the celery and the cell wall of the celery after the pectin components are removed are adsorbed / desorbed, the purity of the polyphenols can be improved by about 5.4 times.

This invention discloses a supercritical fluid-based cell lysis ionization and flow cytometry-mass spectrometry detection device. The device includes a supercritical fluid generator, a cell introduction device, a fluid focusing device, and a mass spectrometer detector. The supercritical fluid generator is connected to the fluid focusing device to introduce supercritical fluid. The outlet of the cell introduction device is connected to the fluid focusing device to introduce a cell dispersion of the target cells into the fluid focusing device. The fluid focusing device, driven by the supercritical fluid, focuses and generates droplets containing single-cell samples. The droplets vaporize after passing through the outlet of the fluid focusing device and are then ionized. The mass spectrometer detector is located at the outlet of the fluid focusing device. This invention solves the problem that intact cells cannot be completely ruptured during traditional electrospray ionization, and that metabolite molecules within the cells cannot be completely released to form gaseous ions. The ion clusters generated by single cells enable highly sensitive detection of the transient signals of single-cell metabolites using a mass spectrometer.