Non-enzymatic dissociation of FFPE tissue and generation of single cells with intact cell surface markers

WO2025193604A1PCT designated stage Publication Date: 2025-09-18VENTANA MEDICAL SYSTEMS INC
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Patent Information

Application Number
PCT/US2025/019180
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-04-19
Filing Date
2025-03-10
Publication Date
2025-09-18

AI Technical Summary

Technical Problem

Existing methods for dissociating formalin-fixed paraffin embedded (FFPE) tissue samples require enzymes, leading to the loss of cell surface markers and incomplete dissociation, hindering downstream analysis such as flow cytometric analysis and biomarker staining.

Method used

A non-enzymatic method for processing FFPE tissue samples to generate intact single cells that retain cell surface biomarkers, using mechanical and filtration processes without enzymes, enabling efficient dissociation and downstream analysis.

Benefits of technology

The method achieves high yields of intact single cells with retained cell surface markers, facilitating effective cytometric analysis and biomarker staining, and generating sequence data for therapeutic target identification.

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Abstract

The present disclosure is directed to a non-enzymatic dissociation method which facilitates the dissociation of one or more formalin-fixed paraffin-embedded tissue samples into dissociated single cells. The present disclosure is also directed to methods of single cell analysis, i.e., methods of analyzing and / or measuring target components (e.g., biomolecules such as, but not limited to, polypeptides, polynucleotides, small molecules, and the like) on or in cells non-enzymatically dissociated from one or more FFPE tissue samples.
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Citation Information

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