PEDV genome analysis method based on next-generation sequencing

A virus genome and analysis method technology, applied in the field of PEDV virus genome analysis based on next-generation sequencing, can solve problems such as inability to accurately identify the gene structure and affect PEDV virus genome splicing, and achieve the effect of avoiding omissions
CN111199772AActive Publication Date: 2020-05-26SHANGHAI PASSION BIOTECHNOLOGY CO LTD
3 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI PASSION BIOTECHNOLOGY CO LTD
Publication Date
2020-05-26

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a PEDV genome analysis method based on next-generation sequencing. The method is characterized by comprising the following steps: downloading a genome sequence and a transcriptsequence of a host pig as a host reference sequence; acquiring a sequencing sequence for removing host pollution; splicing the genome; carrying out homologous comparison on the genome; selecting a comparison result; and taking the compared starting site as a starting site of the gene. Compared with the prior art, the method has the advantages that gene structure can be accurately predicted, and omission is avoided.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of gene detection, in particular to a method for analyzing the genome of PEDV virus based on next-generation sequencing. Background technique

[0002] At present, the existing PEDV analysis methods have two limitations. First, there are some or even a large number of host pig genome sequences in the PEDV virus next-generation sequencing data, and the contamination of the host genome will affect the splicing of the PEDV virus genome. Second, the method of splicing and predicting the gene structure of the virus is mainly based on prediction software such as GeneMarkS. However, due to the overlap of genes in the PDEV virus genome and the phenomenon of ribosomal frameshift inside the gene, the existing gene prediction software cannot accurately identify the correct gene. structure. Contents of the invention

[0003] In order to overcome the above-mentioned defects of the prior art, the object of the present invention i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More