Method for establishing early liver injury model of passive smoking rats and method for screening related genes

A screening method and model building technology, applied in genomics, instruments, biological systems, etc., can solve problems such as increasing the risk of liver fibrosis, and achieve clinically significant, reliable, and precise control effects

Active Publication Date: 2020-01-24
CHINA TOBACCO JIANGXI IND CO LTD
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Studies have shown that smoking can reduce the incidence of primary biliary cirrhosis and increase the risk of liver fibrosis in a dose-dependent manner

Method used

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  • Method for establishing early liver injury model of passive smoking rats and method for screening related genes
  • Method for establishing early liver injury model of passive smoking rats and method for screening related genes
  • Method for establishing early liver injury model of passive smoking rats and method for screening related genes

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Experimental program
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Embodiment

[0023] Establishment of passive smoking rat model

[0024] 1 Experimental animals: animal species: SD rats, SPF grade, male mice, a total of 24, the age of the animals at the time of purchase: 9 to 10 weeks old, the animals were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., license No.: SCXK-(Beijing) 2016-0006. Animals are kept in a barrier environment (permit number: SYXK (Jin) 2013-0002, valid period: December 4, 2013 to December 4, 2018, certification authority: Shanxi Provincial Department of Science and Technology), accredited by the qualification personnel feeding. Use the PeopleSoft environmental monitoring platform to monitor the room temperature and humidity of animal feeding, the room temperature is controlled at 20-26°C, the humidity is 40-70%, 12 hours of lighting and 12 hours of darkness. Raise in the standard rearing cage, every cage 5, change cage 2 times every week. Clean up after the daily operation, wipe the cage frame and...

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Abstract

The invention discloses a method for screening genes related to early liver injury of passive smoking rats. By the method, a rat passive smoking model is established. Through a liver gene chip technology, differential genes of early liver injury in passive smoking rats were screened out, and functional annotation and analysis of the differential genes are performed, thus providing gene-level discovery and explanation for liver injury caused by smoking. The established rat passive smoking model has higher scientificity and stronger reliability, is accurate in controlling the concentration of poisoned smoke, has short modeling period, is of great clinical significance, and can realize early detection and early prevention.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for screening and functional analysis of genes related to liver damage in passive smoking rats. Background technique [0002] It is a well-known fact that smoking is harmful to health. Around the world, 2.5 million people die from smoking every year, and tobacco is the number one killer of human beings. Consciously developing a personal hygiene habit of not smoking is not only beneficial to health, but also a manifestation of noble public health ethics. In the cold smoke that the smoker spits out, the content of tar and nicotine is 1 times more than that of the hot smoke inhaled by the smoker, 2 times more benzopyrene, 4 times more carbon monoxide, and 50 times more ammonia. [0003] Tobacco has been identified as a primary carcinogen by the state. Smokers are 10 to 30 times more likely to suffer from lung cancer than non-smokers, and 90% of the total mortality rate is ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B5/00G16B20/20G16B40/00
CPCG16B5/00G16B20/20G16B40/00Y02A90/10
Inventor 蔡继宝苏加坤徐达郭磊罗娟敏
Owner CHINA TOBACCO JIANGXI IND CO LTD
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