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Method of screening reference genes to fluorescently quantitatively detect oviduct of pregnant cows and application of method

A technology of fluorescent quantitative detection and internal reference genes, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, etc., and can solve the problems of unconfirmed expression stability

Inactive Publication Date: 2019-07-05
WUHAN ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, it has not been found that any internal reference gene can be stably expressed in all cells, tissues and developmental stages and under various experimental conditions. The function of genes is extremely important
At present, the expression stability of commonly used internal reference genes in the fluorescent quantitative detection of pregnant cow oviduct tissue has not been confirmed

Method used

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  • Method of screening reference genes to fluorescently quantitatively detect oviduct of pregnant cows and application of method
  • Method of screening reference genes to fluorescently quantitatively detect oviduct of pregnant cows and application of method
  • Method of screening reference genes to fluorescently quantitatively detect oviduct of pregnant cows and application of method

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Embodiment Construction

[0036] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0037] The embodiment of the present invention provides an internal reference gene screening method for the quantitative detection of the fluorescence of the fallopian tubes of pregnant dairy cows, comprising the following steps:

[0038] S1, extract the RNA of oviduct tissue, and perform reverse transcription;

[0039] (1) Pretreatment of experimental animals:

[0040] The 3 test cows came from a standardized dairy cow demonstration farm in Wuhan, with normal reproductive system and fertility, and they were all multi-parity cows. Estrus synchronous drugs follicle-stimulating hormone (FSH), loprostinol (PG) and injection of gonadorelin (GnRH) were purchased from Ningbo Sansheng Pharmaceutical Co., Ltd. On the 9th day of the estrous cycle of the test cows, superovulation treatment was carried out, and follicle-stimulating hormone (FS...

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Abstract

The invention discloses a method of screening reference genes to fluorescently quantitatively detect oviduct of pregnant cows and application of the method and relates to the technical field of animalmolecular biology. Suitable reference genes for the study on genetic expression of oviduct tissue of pregnant cows; the accuracy of fluorescent quantitative detection of the genetic expression of oviduct of the is forcibly guaranteed; important basis is provided for the further study on the regulatory mechanism of the oviduct of cows in early embryonic development phase.

Description

technical field [0001] The invention relates to the technical field of animal molecular biology, in particular to an internal reference gene screening method for quantitative detection of oviduct fluorescence of pregnant dairy cows and its application. Background technique [0002] The calving rate of dairy cows will directly affect its milk production traits, and is closely related to the economic benefits of dairy farming, so it has received extensive attention from relevant technical personnel in the dairy industry. According to reports, 20-50% of high-yield dairy cows may experience pregnancy failure within 1 week after artificial insemination, which is also an important factor for the decline in calving rate of high-yield dairy cows. Normally, 10 to 15 hours after the end of estrus, the ovary ovulates on one side of the cow, and the egg enters the umbrella of the fallopian tube and goes down the fallopian tube until the ampulla combines with the sperm to complete the fe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6811C12Q1/6888
CPCC12Q1/6811C12Q1/6888C12Q2600/166C12Q2600/124
Inventor 程蕾胡修忠夏瑜余婕向敏周源陶弼菲王定发熊海谦
Owner WUHAN ACADEMY OF AGRI SCI
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