Oil field hydrogen sulfide producing bacterium culture medium and application

A hydrogen sulfide and bacteria-producing technology, applied in microorganisms, microorganisms, isolated microorganisms, etc., can solve the problem of lack of a variety of hydrogen sulfide-producing bacteria culture medium, and achieve the effect of guiding water quality management, improving pertinence and effectiveness

Active Publication Date: 2018-04-20
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a lack of media suitable for a variety of hydrogen sulfide producing bacteria

Method used

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  • Oil field hydrogen sulfide producing bacterium culture medium and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation steps of the hydrogen sulfide producing bacteria medium suitable for medium salinity water quality:

[0023] (1) Preparation of basal medium solution. Accurately weigh 5.5g NaCl, 1.2g MgCl 2 ·6H 2 O, 0.05gCaCl 2 2H 2 O, 4.5g Na 2 SO 4 , 0.2g NH 4 Cl, 0.2g KH 2 PO 4 and 0.5g KCl, dissolved in turn to a final volume of 1000mL in distilled water, then heated to boil, allowed to cool under the protection of high-purity nitrogen, and transferred to an anaerobic bottle with a butyl rubber stopper. Place it at 121°C for 20 minutes for steam sterilization, and let it cool for later use;

[0024] (2) Preparation of carbon source solution. Accurately weigh 10.0g sodium lactate, 10.0g sodium acetate, 5.0g sodium formate, 5.0g sodium propionate, 5.0g sodium butyrate, 5.0g sodium succinate, 5.0g sodium malate, 5.0g sodium fumarate and dissolve them in 100mL distilled water . The anaerobic sterilization method of the carbon source solution is the same as t...

Embodiment 2

[0033] The preparation of the hydrogen sulfide producing bacteria culture medium suitable for higher salinity water quality: when preparing the hydrogen sulfide producing bacteria basal culture medium in the preparation example 1, the consumption of NaCl is increased to 12.0g / L, the formula composition of the remaining solution and The mixing ratio of each solution was not changed, and the final pH of the medium was adjusted to 7.0.

Embodiment 3

[0035] The preparation of the hydrogen sulfide producing bacteria culture medium applicable to the salinity water quality of offshore seawater: when preparing the hydrogen sulfide producing bacteria basal culture medium in Example 1, the consumption of NaCl was increased to 20.0g / L; Sodium lactate was reduced to 5.0g / L, sodium acetate was reduced to 5g / L, and the dosage of sodium formate, sodium propionate, sodium butyrate, sodium succinate, sodium malate, and sodium fumarate was reduced to 1.0g / L. The formulation components of the remaining solutions and the mixing ratio of each solution were unchanged, and the final pH of the medium was adjusted to 7.2.

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Abstract

The invention belongs to the fields of environmental microbiology, microbiological detection and quantity monitoring, and relates to a culture medium formula ingredient for effectively meeting hydrogen sulfide producing bacterium growth and promoting metabolism and reproduction and application thereof. The culture medium is prepared from basic culture medium solution, microelement liquid, vitaminliquid and the like, and also contains nutritional ingredients such as compound organic carbon sources and sulfur sources. Under the anaerobic condition, a proper volume of anaerobic collected oil field environment water samples (such as produced water) are taken and are inoculated into the culture medium; anaerobic culture is performed under the condition with the same temperature as the environment sample in-situ temperature; the growth and the metabolism of various microorganism physiological populations with hydrogen sulfide producing capability in the samples can be effectively promoted.When the culture medium is used, and meanwhile, other anaerobic microbiological methods are combined, the strain enrichment, the separation and screening and the microbiological quantity detection andmonitoring of the hydrogen sulfide producing bacterium in the samples from oil field environment or other environment can be effectively realized.

Description

technical field [0001] The invention belongs to the fields of environmental microbiology, microbial detection and quantity monitoring, and relates to a medium formula component suitable for effectively satisfying the growth of hydrogen sulfide-producing bacteria and promoting its metabolism and reproduction, and an application thereof. Background technique [0002] As far as industrial industries such as oil exploitation are concerned, hydrogen sulfide (H 2 S) brings many hazards to industrial production: (1) H 2 S can cause harm to the personal health and even life safety of personnel. When H 2 When the concentration of S is high (50-200ppm), the gas will poison human organs due to lack of oxygen; death; (2) capable of producing H 2 S microorganisms can also cause microbial corrosion of oil industry pipelines, which can not only cause large economic losses, but also cause safety and production accidents; (3) high concentrations of H 2 S gas will reduce the economic val...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/02C12Q1/06
CPCC12N1/00C12N1/02C12Q1/06
Inventor 史荣久赵峰张颖韩斯琴
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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