Optical tweezers for capturing magnetic particles and preparation method of optical tweezers

Abstract

The invention discloses optical tweezers for capturing magnetic particles and a preparation method of the optical tweezers. The preparation method comprises the following steps: grinding a magnetic metal into powder, cleaning with ethanol, water and acetone in sequence, and carrying out vacuum drying for 2-8 hours; dispersing the metal powder and tetraethoxysilane into a mixed solvent of ethanol and water in a volume ratio ranging from (1:10) (10:1); after 2-30 minutes of dispersion, putting an ethanol solution of ammonia water into the system, wherein the ethanol solution of the ammonia watercomprises ammonia water and ethanol of 3-15% by mass; carrying out a reaction for 1-12 hours at the room temperature, separating the product by using a magnet, washing with ethanol, water and acetonein sequence, and carrying out vacuum drying for 1-12 hours at 36-100 DEG C so as to obtain a product; carrying out ultrasonic and mechanical stirring and dispersion on the obtained product into methylbenzene. The nanoparticles prepared by using the preparation method have excellent properties of magnetism, bioactivity and fluorescence, and have wide application prospects in biological medicine fields of life sciences, genetics, pharmacology and cell imaging.

Description

technical field [0001] The invention relates to the field of biological preparation, in particular to a magnetic particle captured by an optical tweezers device and a preparation method thereof. Background technique [0002] Magnetic microspheres are prepared from magnetic nanoparticles and polymer framework materials. The polymer materials include polystyrene, silane, polyethylene, polyacrylic acid, starch, dextran, gelatin, albumin, cellulose, etc. There are natural materials as well as synthetic materials, which can be used alone or in combination as a skeleton material. These skeleton materials have stable properties, high strength, and no toxic side effects, so they can be called immunomagnetic microspheres. Both experimental research and clinical application have shown that immunomagnetic microspheres are playing a huge role in biomedicine and drug release. The surface-modified nano-magnetic particles are widely used in cell separation, immunoassay, enzyme assay, ma...

AI Technical Summary

Problems solved by technology

And it is more aimed at the motion control algorithm of larger particles such as cells with a larger field of view, and there are not many algorithms required for measurement applications

For the optical tweezers system used for force spectrum measurement, the required particle diameter is smaller, generally around 1-2um; the required optical trap has a higher stiffness, which often needs to reach 0.5pn / nm, which means that the used objective lens With a higher numerical aperture, the observation field of view and depth of field become limited, which ultimately makes the phenomenon of particle recapture more likely to occur

At the same time, due to the increase in stiffness, higher laser power is required, which also leads to a more obvious heating effect in the sample cell and leads to convection of the liquid in the sample cell

In addition to Brownian motion, particles are also affected by convection in the liquid environment, making it more difficult to capture particles in a targeted manner

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