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Verticillium wilt resistance-related molecular markers coming from sea island cotton sea 1, and application of molecular markers

A molecular marker, verticillium wilt technology, applied in applications, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of low efficiency, high cost, long cycle, etc., achieve clear selection goals, save costs, improve The effect of verticillium wilt resistance

Active Publication Date: 2018-06-08
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome many shortcomings of traditional breeding such as poor accuracy of phenotypic selection, low efficiency, long cycle and high cost, and solve the problem of slow progress in breeding for improved Verticillium wilt resistance

Method used

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  • Verticillium wilt resistance-related molecular markers coming from sea island cotton sea 1, and application of molecular markers
  • Verticillium wilt resistance-related molecular markers coming from sea island cotton sea 1, and application of molecular markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1 Screening Molecular Markers

[0060] (1) Construction of substitution lines and acquisition of phenotypic data

[0061] Using sea-island cotton line Hai 1 with excellent fiber and high resistance to Verticillium wilt as the donor parent, and Zhongmiansuo 36, a precocious and high-yielding upland cotton variety susceptible to Verticillium wilt as the recurrent parent, a land-sea hybrid high-generation backcross was constructed. introgression group. China Cotton Research Institute 36 (CCRI36) is an early-maturing upland cotton variety (National Approved Cotton 990007) cultivated by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences.

[0062] Planted 133 BC in 2009 5 f 3 For the family line, 2660 individual plants were harvested, and the natural bolls were collected according to the individual plant, planted in rows, mixed harvest, planted into lines, and identified for Verticillium wilt resistance. Choose 300 BC according to the le...

Embodiment 2

[0085] Example 2 Molecular Marker Selection Method for Upland Cotton Verticillium Wilt Resistance Trait Improvement

[0086] Use the molecular markers CER0086, NAU3405 and NAU2026 obtained in Example 1 to carry out molecular marker selection in the breeding population related to Sea Island Cotton Sea 1, etc., including the following steps:

[0087] (1) DNA extraction: take Sea Island Cotton Sea 1 as the donor parent, and upland cotton varieties or strains (such as Zhongmian Institute 60, Lumianyan 28) as the recipient parent, carry out hybridization and backcrossing to obtain segregated populations, or use Haidao Mianhai 1 is the donor parent, and the upland cotton varieties (such as Zhongmianyan 28, Xinluzao 60) are the substitution lines and their derivative lines obtained by the high-generation backcrossing of the recipient parents, or other The offspring population of the hybridization and backcrossing of the substitution line and the upland cotton variety, the DNA of a si...

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Abstract

The invention relates to the technical field of breeding, and discloses verticillium wilt resistance-related molecular markers coming from sea island cotton sea 1, and application of the molecular markers. The molecular markers are respectively CER0086, NAU3405 and NAU2026. The molecular markers provided by the invention can help to overcome the defects of the existing breeding technique in the identification of verticillium wilt resistance, and can improve the selection efficiency of the verticillium wilt resistance, thus accelerating the cultivation process of new varieties with resistance to verticillium wilt.

Description

technical field [0001] The invention relates to the technical field of cotton molecular breeding, in particular to a molecular marker related to Verticillium wilt resistance from Sea Island Cotton Sea 1 and its application. Background technique [0002] Cotton verticillium wilt (Verticillium wilt) is a soil-borne disease that infects vascular tissue caused by Verticillium dahliae Klebahn. Cancer” poses a serious threat to the cotton planting industry in major cotton-producing countries and regions in the world (Ma Cun, 2005.). In recent years, there is a trend of increasing year by year in China's cotton-producing areas. Therefore, it is imperative to cultivate new cotton varieties resistant to Verticillium wilt. [0003] Sea-island cotton has high resistance to Verticillium wilt, but its yield is low and the planting area is small, while upland cotton has high yield, wide adaptability, and is planted in a large area, but it is sensitive to Verticillium wilt. Therefore, e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11A01H1/04
CPCA01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 石玉真袁友禄李鹏涛巩万奎李俊文商海红葛群龚举武刘爱英王艳玲
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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