Molecular markers derived from sea island cotton Hai 1 and associated with fiber length and applications of molecular markers

A molecular marker and fiber length technology, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc. The effect of industrialization process, slow progress in solving, and clear selection of goals

Active Publication Date: 2015-12-30
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to overcome many shortcomings such as poor accuracy of phenotypic selection, long cycle and low efficiency in traditional breeding, and solve the problem of slow progress in fiber quality breeding

Method used

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  • Molecular markers derived from sea island cotton Hai 1 and associated with fiber length and applications of molecular markers
  • Molecular markers derived from sea island cotton Hai 1 and associated with fiber length and applications of molecular markers
  • Molecular markers derived from sea island cotton Hai 1 and associated with fiber length and applications of molecular markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Screening Molecular Markers

[0047] (1) Construction of introgression lines and acquisition of phenotypic data

[0048] Using Haidao Mianhai 1 as the donor parent and Zhongmian 36 as the recurrent parent, a high-generation backcross introgression line population of land-sea hybrids was constructed. The recurrent parent Zhongmian Research Institute 36 (CCRI36) is an early-maturing and excellent upland cotton variety (National Approved Cotton 990007) bred by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences, and the donor parent Hai1 (Hai1) is a dominant aglandular gene Sea-island cotton cultivars with high resistance to Verticillium wilt and excellent characteristics such as long, strong and fine fibers.

[0049] In the summer of 2003, F 1 , in the winter of the same year in Sanya, Hainan, backcrossed BC with Zhongmian Institute 36 as the female parent 1 f 1 ; Planted BC in Anyang, Henan in the summer of 2004 1 f 1 , using the...

Embodiment 2

[0082] Example 2 Molecular marker selection method for improving fiber length traits of upland cotton

[0083] Using the molecular marker PGML01861 obtained in Example 1 330 、DPL0209 200 and CGR5565 150 Molecular marker selection is carried out in the breeding population related to Sea Island Cotton Sea 1, etc., including the following steps:

[0084] (1) DNA extraction: use Sea Island Cotton Sea 1 as the donor parent, and upland cotton varieties or strains (such as Lumianyan 28 and Zhongmian Institute 60) as the recipient parent, carry out hybridization and backcrossing to obtain segregated populations, or use Haidao Mianhai 1 is the donor parent, and upland cotton varieties (such as Lumianyan 28 and Zhongmiansuo 60) are the introgression lines and their derivatives obtained by crossing high-generation backcrosses of the recipient parents, or their introgression lines and land cotton varieties. For the offspring populations of hybridization and backcrossing of cotton varie...

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Abstract

The invention relates to the technical field of molecular breeding, in particular to molecular markers derived from sea island cotton Hai 1 and associated with the fiber length and applications of the molecular markers. The molecular markers are PGML01861(330), DPL0209(200) and CGR5565(150). According to the molecular markers and the applications, the defects of conventional breeding technology for fiber quality identification are overcome, the fiber length selection efficiency can be improved, and the cultivation process of a new high-quality variety is accelerated.

Description

technical field [0001] The invention relates to the technical field of cotton molecular breeding, in particular to a molecular marker related to fiber length from Sea Island Cotton Sea 1 and its application. Background technique [0002] Cotton is an important economic crop in the world, and cotton fiber is an important raw material for textile industry. Cotton occupies an important position in my country's national economy. With the rapid development of the textile industry and the continuous improvement of people's living standards, the requirements for the quality of cotton fibers are also getting higher and higher. Before the 1980s, my country's cotton breeders mainly focused on the improvement of yield, but did not pay enough attention to the improvement of fiber quality. As a result, most of my country's cotton varieties showed high yield and poor quality. In the middle and late 1990s, fiber quality breeding has become an important goal of cotton molecular breeding. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 石玉真袁友禄檀运娜何蕊张金凤刘爱英李俊文巩万奎王涛龚举武商海红陈婷婷葛群
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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