Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Methods and compositions for inactivating glutamine synthetase gene expression

A gene and binding domain technology, applied in cell culture, cell line generation and protein preparation, genome engineering, and can solve problems such as laborious

Active Publication Date: 2014-04-16
SANGAMO BIOSCIENCES INC
View PDF78 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These targeted gene deletion methods require successive cycles of homologous recombination followed by drug selection to isolate rare desired events - a process that is laborious enough to limit application to individual genetic loci

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods and compositions for inactivating glutamine synthetase gene expression
  • Methods and compositions for inactivating glutamine synthetase gene expression
  • Methods and compositions for inactivating glutamine synthetase gene expression

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0114] Preparation of lipid:nucleic acid complexes, including targeting liposomes, such as immunolipid complexes, is well known to those of ordinary skill in the art (see, e.g., Crystal, Science 270:404-410 (1995); Blaese et al. al., Cancer Gene Ther. 2: 291-297 (1995); Behr et al., Bioconjugate Chem. 5: 382-389 (1994); Remy et al., Bioconjugate Chem. 5: 647-654 (1994); Gao et al., Gene Therapy 2: 710-722 (1995); Ahmad et al., Cancer Res. 52: 4817-4820 (1992); , 4,837,028 and 4,946,787).

[0115] RNA or DNA delivery systems for nucleic acids encoding engineered ZFPs take advantage of the highly evolved process of targeting viruses to specific cells in vivo and transporting the viral payload to the nucleus. Viral vectors can be administered directly to a patient (in vivo), or can be used to treat cells in vitro and administer the modified cells to a patient (ex vivo). Conventional viral systems for ZFP delivery include, but are not limited to, retroviruses, lentiviruses, aden...

Embodiment 1

[0137] Example 1: Engineering and Structure of ZFNs

[0138] A. Glutamine Synthetase (GS) ZFN

[0139] Because the full sequence of the CHO genome is not available, the CHO GS gene was cloned and sequenced to generate the target DNA sequence for ZFN engineering. Figure 13 shows the complete CHO GS sequence, with introns and exons also specified.

[0140] The ZFN target site within exon 6 of the CHO GS gene was chosen because this region encodes amino acids critical to the catalytic function of the GS protein based on available crystal structures (see, e.g., Almassy et al. (1986) Nature 323:304-309; Gill et al. (2002) Biochem.41:9863-9872; Liaw et al. (1994) Biochem.33:675-681), as such, ZFN-mediated mutations at this site are expected to cause Reduction of functional GS activity. The partial sequence of CHO GS exon 6 is shown below. Capital letters indicate exon sequences; lowercase letters indicate intron sequences; ZFN target site 9372 / 9075 (Table 1, Figure 1A and F...

Embodiment 2

[0155] Example 2: GS-ZFN modification of endogenous GS

[0156] To determine whether GS targeted the ZFN-modified endogenous GS locus as expected, a CEL-1 mismatch assay was performed essentially following the manufacturer's instructions (Trangenomic SURVEYOR™). Briefly, appropriate ZFN plasmid pairs were transfected into CHO K-I cells grown adherently in serum-containing medium or CHO-S cells grown in suspension in chemically defined medium without serum.

[0157] CHO K-I cells were obtained from the American Type Culture Collection and are recommended for growth in F-12 medium (Invitrogen) supplemented with 10% qualified fetal calf serum (FCS, Hyclone). CHO-S cells were purchased from Invitrogen (Carlsbad, CA) and grown in chemically defined protein- and animal-component-free CD-CHO medium maintained in suspension culture at 37°C, containing 5% carbon dioxide, at 125 rpm in a humidity-controlled shaking incubator (ATR, inc., Laurel, Maryland) supplemented with 8 mM L-glut...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Disclosed herein are methods and compositions for inactivating the glutamine synthetase (GS) gene using a fusion protein comprising a zinc finger protein and a cleavage domain or half-domain. Also provided is a polynucleotide encoding the fusion protein, a cell comprising the polynucleotide and the fusion protein.

Description

[0001] Statement of Invention Rights Under Federally Sponsored Research [0002] Not applicable. technical field [0003] The present invention relates to the fields of genome engineering, cell culture, cell line production and protein production. Background technique [0004] Glutamine synthetase (GS) is a key enzyme in the synthesis of the amino acid L-glutamine. See, Meister, A. in Glutamine Metabolism, Enzymology and Regulation (eds. J. Mora & R. Palacios) 1-40 (Academic Press, N.Y.; 1980). Thus, GS negative cell lines are auxotrophic for L-glutamine. GS is often used as a selectable marker gene in CHO cell-based recombinant protein expression systems, although selection using the GS inhibitor methionine sulfoxime is required in the absence of GS-negative CHO cell lines (Wurm et al. (2004) Nature Biotechnology 22:1393-1398). [0005] Furthermore, dihydrofolate reductase (DHFR, 5,6,7,8-tetrahydrofolate: NADP+ oxidoreductase) is an essential enzyme in eukaryotes and pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/00C12Q1/16C12N15/11
CPCC12N9/93C12N2510/00C12P21/02C12N9/22C12Y603/01002C12N15/62C07K19/00C12N15/85Y02P20/52C07K14/00
Inventor 刘佩琪杰弗里·C·米勒
Owner SANGAMO BIOSCIENCES INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com