Tea tree glutamine synthetase gene SSR molecular marker primer and application

A technology of molecular markers and glutamine, applied in biochemical equipment and methods, microbe determination/testing, DNA/RNA fragments, etc., can solve unreported problems

Active Publication Date: 2022-05-13
GUANGXI SUBTROPICAL CROPS RES INST GUANGXI SUBTROPICAL AGRI PROD PROCESSING RES INST
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Problems solved by technology

The GS genes of some tea trees such as Anji white tea have also been cloned, but the development and application of SSR molecular marker primers for the glutamine synthetase (GS) gene of tea trees have not been reported yet.

Method used

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  • Tea tree glutamine synthetase gene SSR molecular marker primer and application
  • Tea tree glutamine synthetase gene SSR molecular marker primer and application

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Effect test

Embodiment Construction

[0026] Study on SSR Molecular Marker of Glutamine Synthetase (GS) Gene in Tea Tree Theanine Synthesis Pathway

[0027] (1) Download the gene sequence of the glutamine synthetase (GS) gene (GenBank: JQ925873) in the theanine synthesis pathway of tea tree from NCBI.

[0028] (2) Download the whole genome sequence of the tea tree 'Yunkang No. 10' (download address: http: / / www.plantkingdomgdb.com / tea_tree / dat). Use the SSR search software to find the SSR markers in the whole genome of tea tree, and extract the SSR sequence and its upstream and downstream sequences of specific length (100bp by default). Use primer3 to design primers to obtain primer sequences, SSR repeat motifs and repeat lengths, and pre-amplified fragment lengths. Based on this, 50 pairs of SSR molecular marker primer sequences related to the glutamine synthetase gene were synthesized and prepared.

[0029] (3) The HYQspin TM CT Plant DNA Kit produced by Tiangen Biological Co., Ltd. was used to extract th...

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Abstract

The invention discloses a tea tree glutamine synthetase gene SSR molecular marker (SSR1-GS, SSR2-GS) primer and application of the tea tree glutamine synthetase gene SSR molecular marker primer. Researches show that the method for developing the SSR primer on the whole genome of the tea tree according to specific genes is feasible, and the obtained primer is high in polymorphism and good in repeatability and has good feasibility and pertinence. Therefore, the inventor develops research on genetic polymorphism of tea tree germplasm resources. In conclusion, the research method, the SSR molecular marker and the primer of the SSR molecular marker can be widely applied to tea tree variety identification, genetic structure and resource diversity analysis, genetic map construction, functional gene positioning and QTL positioning, seed purity identification and molecular marker-assisted seed selection research, and tea tree theanine synthesis and tea tree genetic polymorphism can be further researched conveniently; deep development of tea tree resources is promoted.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, in particular to SSR molecular marker primers and applications of tea tree glutamine synthetase (GS) gene. Background technique [0002] Tea tree is an important woody economic crop in China. Theanine (γ-N-ethyl-glutamine) is a unique free amino acid in tea tree. It is not only the basic unit of protein, but also active peptide, enzyme and some other biological The important constituents of active molecules are the main constituents of the tea that produces body fluids and moisturizes sweetness. Amino acid is involved in tea aroma during tea processing, and the volatile aldehydes or other products it transforms into are the components of tea aroma. The composition and content of amino acids in tea, as well as their degradation products and transformation products directly affect the quality of tea. [0003] Glutamine synthetase (GS, EC 6.3.1.2) is a key enzyme in the process of nitrog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2600/13C12Q2531/113C12Q2565/125
Inventor 彭靖茹黄寿辉温立香张芬陈家献檀业维赵媛袁冬寅甘志勇冯春梅李建强黎新荣
Owner GUANGXI SUBTROPICAL CROPS RES INST GUANGXI SUBTROPICAL AGRI PROD PROCESSING RES INST
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