Gastrodia elata glutamine synthetase gene and application thereof

Abstract

The invention discloses a gastrodia elata Glutamine Synthetase (GS) gene. The nucleotide sequence of the gene is shown as SEQ ID NO: 1; and 342 amino acid residues are encoded; a prokaryotic expression vector of the gastrodia elata GS gene is constructed, and prokaryotic expression analysis shows that the gastrodia elata glutamine synthetase gene is soluble protein, and the molecular weight of the gene is about 59.94kDa. The eukaryotic overexpression vector of the gastrodia elata glutamine synthetase gene is constructed, gastrodia elata symbiotic bacteria armillaria mellea is transfected through an agrobacterium method, the armillaria mellea transfected with the gastrodia elata glutamine synthetase gene is obtained, the GS genetically engineered armillaria mellea is cultured at 13 DEG C and 28 DEG C, the growth vigor of the GS genetically engineered armillaria mellea is better than that of wild type armillaria mellea, and the growth speed is high. Therefore, the gastrodia elata glutamine synthetase gene is beneficial for shortening the culture time of the armillaria mellea and improving the cold resistance and high-temperature growth resistance of the armillaria mellea. The gastrodia elata glutamine synthetase gene provides an experimental basis for enhancing the cold resistance of gastrodia elata, expanding the planting distribution range of gastrodia elata, enhancing the nitrogen metabolism capacity of gastrodia elata and increasing the yield of gastrodia elata.

Description

technical field [0001] The invention belongs to the related technical fields of molecular biology and genetic engineering, and relates to a glutamine synthetase gene in Gastrodia elata and its application in improving the cold resistance of Gastrodia elata. Background technique [0002] Tianma ( Gastrodia elata ) is a heterotrophic perennial herb that coexists with Armillaria mellea, and is distributed in most parts of the country; its dry tubers, also known as Gastrodia elata, are commonly used and relatively expensive traditional Chinese medicine. Epilepsy, convulsions, tetanus and other diseases, Gastrodia elata has very high economic and medicinal value, and the market demand is large. Gastrodia elata has always relied on wild resources in the past. Since the successful transformation from wild to artificial cultivation in the 1970s, artificial cultivation of Gastrodia elata has become the main source of commodities. Gastrodia elata seed germination is different from o...

AI Technical Summary

Problems solved by technology

There are many factors that affect the growth and development of Gastrodia elata, such as environment, temperature, soil, harvesting, etc. Among them, temperature is the key factor affecting the growth of Gastrodia elata; Gastrodia elata is very sensitive to temperature, and when the temperature is too high or too low, it will inhibit the growth of Gastrodia elata; The most suitable temperature for the growth of Gastrodia elata is 20~25°C; when the temperature is below -4°C, Gastrodia elata is susceptible to freezing damage and loses its reproductive ability; when the temperature is -4~10°C, Gastrodia elata stops growing and enters the low-temperature dormancy period; At ~14°C, the underground tubers of Gastrodia elata begin to germinate and grow; when the temperature rises to 20°C, Gastrodia elata enters a rapid growth period, but when the temperature exceeds 30°C, the growth of Gastrodia elata will be inhibited, thereby affecting the yield

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