Method for producing long-chain binary acid through fermentation separation coupling

Abstract

The invention discloses a method for producing long-chain binary acid through fermentation separation coupling, in particular to a method of high-yield dodecanedioic acid (DC12). According to the technical scheme, the method includes the steps that candida viswanathii is cultivated into a seed solution which is led into 5-40% (v / v) n-alkane and 60-95% (v / v) liquid fermentation medium mixture, wherein the pH of the n-alkane ranges from 5.0 to 8.5, and 10 to 18 carbon atoms are contained in the n-alkane; a saccharic multielement substrate serves as a growth carbon source of the liquid fermentation medium mixture; mixing liquid is cultivated for 42 h to 194 h under the conditions that the temperature ranges from 24 DEG C to 40 DEG C, and the air passing amount ranges from 0.1 vvm to 3.0 vvm, a centrifugal fermentation separation coupling device or a plate frame filtering fermentation separation coupling device is started, cells passing through the separation coupling device are circulated back to a fermentation cylinder, and clear liquid passing through the separation coupling device enters an extraction link to prepare long-chain binary acid; and meanwhile the fermentation process is continuously carried out on a cultivation medium in a fermentation tank. The method is used for converting n-dodecane so as to produce the DC12, the produced acid reaches 240 g / L, and the acid production rate is larger than 1.5 g / h.L.

Description

technical field

[0001] The technical solution of the invention belongs to the technical field of fermentation engineering, and specifically relates to a method for producing long-chain dibasic acids through fermentation, separation and coupling. Background technique

[0002] Long-chain dibasic acids are important raw materials for the synthesis of fragrances, engineering plastics, hot-melt adhesives, coatings, lubricating oils, resins and medicines.

[0003] At present, long-chain dibasic acids are mainly produced by microbial fermentation, and the special function of microbial double-end oxidation is used to ferment n-alkanes to produce long-chain dibasic acids. At present, the ω-oxidation ability of domestic and foreign production strains is strong, and the acid production can reach 200g / L. However, a primary problem in the process of producing long-chain dibasic acids is low fermentation efficiency. Chinese patent CN1233658A discloses a method for screening Candida tropi...