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Method for producing long-chain binary acid through fermentation separation coupling

A technology of fermentation separation coupling and long-chain dibasic acid, which is applied in the field of fermentation engineering, can solve the problems of cell inactivation and other problems, achieve the effect of reducing product inhibition, increasing cell density, and realizing recycling

Active Publication Date: 2015-08-26
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-chain dibasic acids need to be pretreated on the fermentation broth before extraction. For example, in patent CN1570124A, the fermentation broth is added with alkali to adjust the pH to 8-12, heated to 60-100°C, and then the demulsification and layering method is used to remove the fermentation broth. Bacteria and residual fatty acids, the bacteria isolated in this process lose their activity and cannot be reused in the fermentation process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] This embodiment is a fermentation process without adding a fermentation and separation coupling device. As a comparison of adding a fermentation and separation coupling device, the process of fermenting and synthesizing long-chain dibasic acids is as follows:

[0018] The first step, culture medium preparation

[0019] ① Incline medium: malt juice agar medium;

[0020] ②Seed medium: glucose 20g / L, disodium hydrogen phosphate 2g / L, yeast extract 1g / L, corn steep liquor 2g / L, urea 0.5g / L, n-dodecane 100mL / L;

[0021] ③Fermentation medium: glucose 20g / L, disodium hydrogen phosphate 2g / L, yeast extract 1g / L, corn steep liquor 2g / L, urea 0.5g / L, NaCl 1g / L, KCl 1g / L, n-dodecane 300mL / L L, Tween 801g / L.

[0022] The second step, the activation of bacteria

[0023] Take a parent Candida viswanathii ipe-1 spread on a 20×180mm large test tube solid slant medium, and culture at 27°C for 72h.

[0024] The third step, seed cultivation

[0025] The activated seed culture on the ...

Embodiment 2

[0029] Follow the steps below to ferment and synthesize long-chain dibasic acids:

[0030] The first step, culture medium preparation

[0031] Same as Example 1

[0032] The second step, the activation of bacteria

[0033] Same as Example 1

[0034] The third step, seed cultivation

[0035] Same as Example 1

[0036] The fourth step, inoculation and fermentation

[0037] The culture obtained in the third step was inoculated into a 7.5L fermenter with 3L fermentation medium at an inoculum size of 10%, the initial pH was 6.0, the temperature was 27°C, and the ventilation rate was 1.0vvm, and cultured for 42h. Start the fermentation separation coupling device so that the bacterial cells flow back into the fermenter through the coupling device, and the clear liquid after passing through the coupling device enters the extraction process. When the DC concentration in the fermenter is less than 10g / L, reduce the rate of clear liquid extraction; when the DC in the fermenter is >...

Embodiment 3

[0039] Follow the steps below to ferment and synthesize long-chain dibasic acids:

[0040] The first step, culture medium preparation

[0041] Same as Example 1

[0042] The second step, the activation of bacteria

[0043] Same as Example 1

[0044] The third step, seed cultivation

[0045] Same as Example 1

[0046] The fourth step, inoculation and fermentation

[0047] The culture obtained in the third step was inoculated into a 7.5L fermenter with 3L of fermentation medium at an inoculum size of 10%, the initial pH was 6.0, the temperature was 27°C, and the ventilation rate was 1.0vvm, and cultured for 194h. Start the fermentation separation coupling device so that the bacterial cells flow back into the fermenter through the coupling device, and the clear liquid after passing through the coupling device enters the extraction process. When the DC concentration in the fermenter is less than 10g / L, reduce the rate of fermentation broth extraction; when the DC in the fermen...

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Abstract

The invention discloses a method for producing long-chain binary acid through fermentation separation coupling, in particular to a method of high-yield dodecanedioic acid (DC12). According to the technical scheme, the method includes the steps that candida viswanathii is cultivated into a seed solution which is led into 5-40% (v / v) n-alkane and 60-95% (v / v) liquid fermentation medium mixture, wherein the pH of the n-alkane ranges from 5.0 to 8.5, and 10 to 18 carbon atoms are contained in the n-alkane; a saccharic multielement substrate serves as a growth carbon source of the liquid fermentation medium mixture; mixing liquid is cultivated for 42 h to 194 h under the conditions that the temperature ranges from 24 DEG C to 40 DEG C, and the air passing amount ranges from 0.1 vvm to 3.0 vvm, a centrifugal fermentation separation coupling device or a plate frame filtering fermentation separation coupling device is started, cells passing through the separation coupling device are circulated back to a fermentation cylinder, and clear liquid passing through the separation coupling device enters an extraction link to prepare long-chain binary acid; and meanwhile the fermentation process is continuously carried out on a cultivation medium in a fermentation tank. The method is used for converting n-dodecane so as to produce the DC12, the produced acid reaches 240 g / L, and the acid production rate is larger than 1.5 g / h.L.

Description

technical field [0001] The technical solution of the invention belongs to the technical field of fermentation engineering, and specifically relates to a method for producing long-chain dibasic acids through fermentation, separation and coupling. Background technique [0002] Long-chain dibasic acids are important raw materials for the synthesis of fragrances, engineering plastics, hot-melt adhesives, coatings, lubricating oils, resins and medicines. [0003] At present, long-chain dibasic acids are mainly produced by microbial fermentation, and the special function of microbial double-end oxidation is used to ferment n-alkanes to produce long-chain dibasic acids. At present, the ω-oxidation ability of domestic and foreign production strains is strong, and the acid production can reach 200g / L. However, a primary problem in the process of producing long-chain dibasic acids is low fermentation efficiency. Chinese patent CN1233658A discloses a method for screening Candida tropi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/72
CPCC07C51/42C12P7/6409C07C55/21
Inventor 万印华曹伟锋杭晓风陈向荣
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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