34results about How to "High feeding concentration" patented technology

The invention relates to a preparation method of chitosan nano particles embedded with alpha-galactosidase in the technical field of feeding enzymic preparations, which comprises the steps: preparing the chitosan stock solution; adding alpha-galactosidase crude enzyme solution with the same volume into the chitosan stock solution, and evenly mixing by adopting the magnetic stirring method to prepare alpha-galactosidase mixed solution; adding sodium tripolyphosphate water solution drop by drop into the alpha-galactosidase mixed solution, and magnetic stirring at room temperature to prepare thechitosan nano particles embedded with alpha-galactosidase. The invention has simple preparation operation and can obtain chitosan nano particles embedded with alpha-galactosidase by the simple mixingof chitosan / alpha-galactosidase / sodium tripolyphosphate; the particles are not adhered, have good ballability and round surfaces, can release enzymic preparations fast at the acid or alkaline condition consistent with the acidity and alkalinity of the stomach and intestines of the pig, and is beneficial to the better efficacy exertion of the feeding alpha-galactosidase.

The invention provides a method for preparing a steroid drug intermediate employing bioconversion phytosterol. The method comprises the following steps: 1) providing turbid liquid of phytosterol; 2) providing a resting cell of a microbial strain for converting the phytosterol into a steroid drug intermediate; 3) mixing the turbid liquid of the phytosterol with the resting cell, and adding beta-cyclodextrin or chemically modified beta-cyclodextrin to evenly mix, and cultivating to finish conversion; 4) centrifuging the conversion liquid obtained in the step 3), separating supernatant from thallus, separating to obtain the steroid drug intermediate from the supernatant by solvent extraction. By adopting the method provided by the invention, the sterol feeding concentration is improved, the target product quantity is increased, simple separation of the thallus, a cosolvent and a product is achieved when the conversion lasting time is shortened, repeated use of the thallus and the cosolvent for a plurality of times is achieved, the production cost is greatly saved, and the method is applicable to industrial production.

This invention relates to a method of using gas containing molecular oxygen to catalytic oxidate double-gan phosphine for preparing glyphosate. The steps:(1) mix double-gan phosphine, water and activated carbon, at condition of speed of agitator 800 to 1500 r / m , inlet oxygen enrichment gas to carry out reaction, reactant liquor for refrigeration, glyphosate seed out, collect glyphosate and activated carbon blending press cake, divide mother solution with deleterious impurities such as formaldehyde; (2) plunge glyphosate and activated carbon admixture press cake to glyphosate saturation solution, after glyphosate dissolving take filtration while hot, filtrate recooling, glyphosate seedout, cake drying, then gain glyphosate solid; or plunge press cake to water, cooling, drop isopropylamine or inlet alkaline air, directly prepare isopropylamine salt or ammonium salt water solution.

The invention discloses a method for preparing 11α, 17α-hydroxyprogesterone by conversion of immobilized hydroxylase, which is characterized in that 11α, 17α-hydroxyprogesterone is prepared by conversion of immobilized hydroxylase with 17α-hydroxyprogesterone as a substrate Hydroxyprogesterone includes preparation of medium, cultivating Aspergillus ochrae in the medium, induction of hydroxylase, preparation of immobilized hydroxylase, preparation of transformation system, conversion of substrate, extraction and purification of products and other steps. The invention has the advantages of high feeding concentration, high conversion rate of substrate, short conversion cycle, easy separation of products and repeated use of immobilized hydroxylase. The feeding concentration of 17α-hydroxyprogesterone in a single enzyme reaction is 40g / L, and the molar conversion rate can reach 93%. At the same time, the immobilized hydroxylase can be used repeatedly for more than 3 times. The invention has simple operation process, low production cost and high industrial application prospect.

This invention relates to a new Rhodotorula.sp used to prepare DCPC by biotransformation method and the method of using this strain to prepare DCPC. One kind of microorganism strain, its features is that the strain is rhodotorula category, its name is Rhodotorula R-92, its number in preservation center is CGMCC NO.1277, strain is used as biotransformation agent in this transform method. Organism transformation substrate is cephalosporin C salt water solution. Transformation agent is added to substrate to transform or blending ferment, then white or tiny yellow DCPC powder is got after resin proposal, concentration and solvent crystallization. The goal of this invention is to solve the problem of low yield and high cost and not fit to industrial scale generating in the generation of DCPC.