34results about How to "High feeding concentration" patented technology

This invention relates to a method of using gas containing molecular oxygen to catalytic oxidate double-gan phosphine for preparing glyphosate. The steps:(1) mix double-gan phosphine, water and activated carbon, at condition of speed of agitator 800 to 1500 r/m , inlet oxygen enrichment gas to carry out reaction, reactant liquor for refrigeration, glyphosate seed out, collect glyphosate and activated carbon blending press cake, divide mother solution with deleterious impurities such as formaldehyde; (2) plunge glyphosate and activated carbon admixture press cake to glyphosate saturation solution, after glyphosate dissolving take filtration while hot, filtrate recooling, glyphosate seedout, cake drying, then gain glyphosate solid; or plunge press cake to water, cooling, drop isopropylamine or inlet alkaline air, directly prepare isopropylamine salt or ammonium salt water solution.

The invention provides a method for preparing a steroid drug intermediate employing bioconversion phytosterol. The method comprises the following steps: 1) providing turbid liquid of phytosterol; 2) providing a resting cell of a microbial strain for converting the phytosterol into a steroid drug intermediate; 3) mixing the turbid liquid of the phytosterol with the resting cell, and adding beta-cyclodextrin or chemically modified beta-cyclodextrin to evenly mix, and cultivating to finish conversion; 4) centrifuging the conversion liquid obtained in the step 3), separating supernatant from thallus, separating to obtain the steroid drug intermediate from the supernatant by solvent extraction. By adopting the method provided by the invention, the sterol feeding concentration is improved, the target product quantity is increased, simple separation of the thallus, a cosolvent and a product is achieved when the conversion lasting time is shortened, repeated use of the thallus and the cosolvent for a plurality of times is achieved, the production cost is greatly saved, and the method is applicable to industrial production.

The invention relates to a method for hydroxylating dehydroisoandrosterone (DHEA) by using colletotrichumlini ST-1. The method for hydroxylating the dehydroisoandrosterone by using the colletotrichumlini ST-1 is characterized in that: in a conversion process, a way of pre-including the DHEA and methyl-beta-cyclodextrin with an equimolar ratio first and then feeding is adopted, so that the water solubility and the conversion efficiency of the DHEA are significantly improved. Under a condition that a substrate feeding amount is 10g/L, the conversion rate is as high as 95% and the total yield of products 7 alpha-OH-dehydroepiandrosterone and 7,15 alpha-OH-dehydroepiandrosterone is 80.94%.

The invention relates to a preparation method of clostridium butyricum embedded chitosan nanoparticles in the technical field of feed additives. The preparation method comprises the following steps: preparing a chitosan stock solution; adding an isopyknic clostridium butyricum bacteria liquid into the chitosan stock solution, uniformly mixing by a magnetic stirring method to prepare a clostridium butyricum mixed liquor; and dropwisely adding a sodium tripolyphosphate aqueous solution into the clostridium butyricum mixed liquor, carrying out magnetic stirring at room temperature so as to prepare the clostridium butyricum embedded chitosan nanoparticles. The preparation method is simple to operate. The clostridium butyricum embedded chitosan nanoparticles can be obtained by simple mixing of chitosan/clostridium butyricum/sodium tripolyphosphate. The particles have advantages of no adhesion, good spheronization and round surface. Under an acid or alkali condition consistent with acid-base value of pig stomach and intestine, clostridium butyricum can be rapidly released, thus helping better perform efficacy of clostridium butyricum for feed.

Belonging to the field of biotechnology, the invention relates to a method of utilizing a coenzyme regeneration and resin in-situ extraction integrated strategy to promote hydroxylation of DHEA (dehydroepiandrosterone) by Colletotrichum lini ST-1. The method is characterized in that in the conversion process, a 10g/L cosubstrate is added to conduct NADPH regeneration, and simultaneously D101 type macroporous adsorption resin is employed for in-situ extraction of the product, thus significantly enhancing the conversion rate of C. lini ST-1 on DHEA. Under the condition of a substrate feeding amount of 10g/L, the conversion rate of the substrate DHEA is 93%, and the yield of 7 alpha, 15 alpha-diOH-DHEA can reach 7.12 g/L.

The invention discloses a process method for producing an intermediate 3,20-diethylene glycol of betamethasone serial products, which belongs to the field of chemical medicaments. The method comprises the following steps: using a cortisone acetate intermediate prowazekii oxide (1) and glycol as raw materials, boron trifluoride and triethyl orthoformate as catalysts, and chloralkane as a menstruum to perform reaction; and concentrating, crystallizing, filtering, washing and drying the reactants to obtain a reaction product. The method can increase the batch charging concentration, shorten the reaction time, and greatly improve the yield and the quality of a condensation compound. Besides, the method reduces the equipment loss, avoids the use of benzene solvents which are more harmful, and greatly reduces the harm to health and the pollution to the environment.

The invention discloses ascorbate glucoside crystalline powder and a preparation method thereof, and belongs to the field of biochemical engineering. According to the ascorbate glucoside crystalline powder, by improving the transformation concentration of ascorbate glucoside crystalline, impurities and pigments in a transformation system are removed through a multi-stage membrane separation technology, and the ascorbate glucoside crystalline is separated from ascorbate to further concentrate and recycle the ascorbate; mother liquor is filtered under an acidic environment, and the light transmittance of a product is improved; meanwhile, the problem of browning caused by easy oxidization of the ascorbate glucoside crystalline in the drying process is solved by adopting a vacuum microwave drying technology.

The invention discloses a novel 12-hydroxycholic acid dehydrogenase derived from Eggerthella lenta and an encoding gene of the novel 12-hydroxycholic acid dehydrogenase. The novel 12-hydroxycholic acid dehydrogenase is used as a biocatalyst for catalyzing cholic acid and a derivative 12-hydroxyl thereof for oxidizing into a carbonyl substrate. The gene for encoding the enzyme has low homology withthe currently known 12-hydroxysteroid dehydrogenase, has high enzyme activity on 12-hydroxycholic acid and derivatives thereof, does not need to add antibiotics in the process of culturing and expressing escherichia coli of the gene, does not utilize traditional isopropyl-beta-D-thiogalactopyranoside (IPTG) for induction, and efficient expression can be achieved. In the conversion process, afterthalli are concentrated, the substrate concentration can reach 200 g/L and is achieved with the conversion rate greater than 95%, no organic solvent is used in the reaction process, and the conversionmethod is green and environmentally friendly.

The invention discloses a method for preparing 11alpha, 17alpha-hydroxyprogesterone by utilizing immobilized hydroxylase conversion, which is characterized in that 17alpha-hydroxyprogesterone is used as a substrate, the 11alpha, 17alpha -hydroxyprogesterone is prepared by utilizing the immobilized hydroxylase conversion, and the method comprises the following steps of: preparing a culture medium, culturing aspergillus ochraceus in the culture medium, inducing hydroxylase, preparing the immobilized hydroxylase, preparing a conversion system, converting a substrate, extracting and refining a product and the like. The method has the advantages that the feeding concentration is high, the substrate conversion rate is high, the conversion period is short, the product is easy to separate, and the immobilized hydroxylase can be repeatedly used. The feeding concentration of 17 alpha-hydroxyprogesterone in a single enzyme reaction is 40 g/L, the molar conversion rate can reach 93%, and meanwhile, the immobilized hydroxylase can be repeatedly recycled for more than three times. The preparation method is simple in operation process and relatively low in production cost, and has a relatively high industrial application prospect.

The invention relates to the field of chemical synthesis, in particular to a DSD acid preparation method. The DSD acid preparation method includes the steps that methylbenzene is subjected to sulfonation, purification and separation to obtain OTS, wherein the byproduct is PTS; OTS is subjected to mixed acid nitration to obtain PNTS; PNTS is subjected to chlorine oxidative condensation to obtain DNS; DNS is subjected to catalyzed hydrogeneration reduction to obtain the high-quality target product, namely DSD acid which can be directly used for synthesizing a fluorescent whitening agent. The synthesis method greatly reduces dangerousness of the process, greatly reduces generation of harmful byproducts and waste, particularly, does not generate a lot of carcinogenic intermediate, namely ortho-nitrotoluene, completely solves the nitration safety problem of the old technology, and has the advantages of being simple in step, high in yield and the like.

The invention belongs to the technical field of biology, and particularly relates to a 5L fermentation tank technique for efficiently converting DHEA (dehydroepiandrosterone) by using Colletotrichum lini ST-1. The technical scheme is as follows: the method comprises the steps of strain activation, seed culture, DHEA bioconversion, product detection and the like. Substrate consumption, residual sugar content and other indexes in the conversion process are monitored, and the batch-by-batch substrate addition is combined with reducing force regeneration promotion to solve the problems of lower substrate feed concentration and lower conversion efficiency. The method increases the product yield, shortens the conversion period, is simple to operate, has high feed amount, and is suitable for large-scale production of steroid compound bioconversion.

The invention relates to an arthrobacter simplex engineered strain with excellent stress tolerance and a construction method and application thereof. The arthrobacter simplex engineered strain is obtained by adopting a promoter engineering method, a global transcription mechanism engineering method or a method combining the promoter engineering and the global transcription mechanism engineering. The strain still keeps good activity in a transformation system with high concentrations of organic solvents (8%) and substrates (15-45 g/L), with the product yield in a C1,2 (subscript) dehydrogenation reaction being 1.1-2.2 times higher than a control strain I (containing a wild-type promoter and a wild-type irrE). According to the construction method and the application of the engineered strainwith excellent stress tolerance, the concentrations of organic solvents and substrates in the transformation system are increased, and then the product production is increased. The invention has important guiding significance for improving the transformation efficiency of the high-concentration substrates of steroid hydrophobic compounds.