Application of nano-liposome technology in bioconversion

A nanoliposome and biotransformation technology, applied in the direction of adding compounds to stimulate growth, etc., can solve the problems of bacterial growth and transformation inhibition, transformation rate decline, etc., to improve water solubility, good biocompatibility, improve Effect of Feed Concentration and Conversion

Inactive Publication Date: 2014-06-04
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of steroid microbial transformation, there is a common phenomenon that with the increase of feed concentration, the conversion rate will decrease significantly, mainly because too much insoluble substrate will inhibit the growth and transformation of bacteria

Method used

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  • Application of nano-liposome technology in bioconversion
  • Application of nano-liposome technology in bioconversion
  • Application of nano-liposome technology in bioconversion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: The 11α hydroxylation reaction of Metarhizium anisopliae 11490 on L-ethyl sterenedione nanoliposomes, with a feed concentration of 2 g / L.

[0053] (1) Preparation of substrate nanoliposomes

[0054] Using lecithin as a carrier, the substrate L-ethyl sterenedione was made into nano liposomes with an average particle size of 131.6 nm.

[0055] (2) Microbial transformation

[0056] Microbial strain: Metarhizium anisopliae 11490, strain deposit number CCTCC M2011240, this strain can introduce 11α hydroxyl on L-ethyl sterenedione.

[0057] (3) Medium

[0058] Slant culture: Potato dextrose agar (PDA) medium, which contains 0.25% of silkworm pupa powder;

[0059] Fermentation medium: 10g / L glucose, 10g / L soybean powder, 5g / L silkworm pupa powder, pH 6.5;

[0060] (4) Microbial culture

[0061] Strains were cultured for 24 hours under the conditions of 220r / min and 40mL / 250mL shake flask on a rotary shaker at 28°C to obtain seed liquid;

[0062] (5) Biotransformation

[0063] Put t...

Embodiment 2

[0065] Example 2: The 11α hydroxylation reaction of Metarhizium anisopliae 11490 on L-ethyl sterenedione nanoliposomes, with a feeding concentration of 4 g / L.

[0066] (1) Preparation of substrate nanoliposomes

[0067] Using lecithin as a carrier, the substrate L-ethyl sterenedione was made into nano liposomes with an average particle size of 131.6 nm.

[0068] (2) Microbial transformation

[0069] Microbial strain: Metarhizium anisopliae 11490, strain deposit number CCTCC M2011240, this strain can introduce 11α hydroxyl on L-ethyl sterenedione.

[0070] (3) Medium

[0071] Slant culture: Potato dextrose agar (PDA) medium, which contains 0.25% of silkworm pupa powder;

[0072] Fermentation medium: 10g / L glucose, 10g / L soybean powder, 5g / L silkworm pupa powder, pH 6.5;

[0073] (4) Microbial culture

[0074] Strains were cultured for 24 hours under the conditions of 220r / min and 40mL / 250mL shake flask on a rotary shaker at 28°C to obtain seed liquid;

[0075] (5) Biotransformation

[0076] Pu...

Embodiment 3

[0078] Example 3: 11α hydroxylation reaction of Metarhizium anisopliae 11490 on L-ethyl sterenedione nanoliposomes, with a feed concentration of 6 g / L.

[0079] (1) Preparation of substrate nanoliposomes

[0080] Using lecithin as a carrier, the substrate L-ethyl sterenedione was made into nano liposomes with an average particle size of 131.6 nm.

[0081] (2) Microbial transformation

[0082] Microbial strain: Metarhizium anisopliae 11490, strain deposit number CCTCC M2011240, this strain can introduce 11α hydroxyl on L-ethyl sterenedione.

[0083] (3) Medium

[0084] Slant culture: Potato dextrose agar (PDA) medium, which contains 0.25% of silkworm pupa powder;

[0085] Fermentation medium: 10g / L glucose, 10g / L soybean powder, 5g / L silkworm pupa powder, pH 6.5;

[0086] (4) Microbial culture

[0087] Strains were cultured for 24 hours under the conditions of 220r / min and 40mL / 250mL shake flask on a rotary shaker at 28°C to obtain seed liquid;

[0088] (5) Biotransformation

[0089] Put the p...

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Abstract

The invention belongs to the technical field of microorganisms and relates to an application of a nano-liposome technology in bioconversion. The application adopts lecithin as a carrier to prepare hydrophobic substrate into nano lipsome, and then carries out microbial conversion. The application is especially applicable to microbial conversion of a hydrophobic compound, or microbial conversion of the substrate which has toxicity to the microbe and inhibiting the growth of the microbe. The application has the advantages that by utilization of the characteristic of the lecithin for increasing the permeability of a cell membrane, the advantages of good biocompatibility of nano liposome and improvement on the water solubility and large special surface area can be exerted, the transportation of the substrate into cells is enhanced, and the amount of the substrate entering the microbial cells, so that the feeding concentration and the conversion rate of the substrate in a conversion system are increased. The invention provides a new method for microbial conversion of the hydrophobic compound.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of nanoliposomes in biotransformation. Background technique [0002] The prior art discloses that the bottleneck that needs to be broken through in practical applications for the conversion of hydrophobic compounds by microorganisms is that the water insolubility of the substrate results in low feeding concentration and low conversion efficiency. Microbial transformation is a two-stage fermentation, which includes: firstly, the microbial strains need to be cultured in a liquid medium for an appropriate period of time (mid or late growth) to accumulate the enzymes required for transformation; then, the substrate to be transformed is put in to start the transformation. The liquid culture of microbial strains is usually carried out in the water phase. The solubility of the substrate in the aqueous solution is directly related to the efficiency of the microbial conv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38
Inventor 叶丽冯美卿
Owner FUDAN UNIV
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