The invention provides an oral ulcer mucoadhesive film and a preparation method thereof. The oral ulcer mucoadhesive film is prepared by honeysuckle flower, menthol, vitamin B [2], borneol, tetracaine hydrochloride, sorbic acid, prednisone acetate, chlortetracycline hydrochloride, glycerin, stevioside, ethanol PVA 17-88 and purified water. Compared with the prior art, the film has good flexibility and tensile strength, can rapidly being expended and dissolved when meets water, a high-density protection drug film layer is formed on the oral cavity mucous membrane, and the effect for rapidly relieving swelling and pain with long term can be reached; oral cavity mucous membrane is employed for direction drug administration, the drug can be directly effected on the disease, simultaneous treatment of principal and subordinate symptoms can be reached, curative effect is fully performed; a lattice impression technology is employed on the technology, administration with divided dose is accurate, curative effect is increased, quality is stable, the oral ulcer mucoadhesive film has no toxic and side effect for oral cavity, and is safe and effective; and has the advantages of small volume, light weight, and convenient carrying and usage.