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Clostridium butyricum embedded chitosan nanoparticles and its preparation method

A technology for embedding Clostridium butyricum and chitosan nanometers, applied in applications, animal feed, animal feed, etc., can solve the problems of heat resistance, unreported, and storage intolerant, and achieve surface roundness and preparation Simple operation and good ball forming effect

Inactive Publication Date: 2013-10-23
杭州银冠海洋生物技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the preparation of composite nanoparticles by embedding Clostridium butyricum with chitosan as a carrier. The composite nanoparticles release Clostridium butyricum quickly under acidic (such as pH 4.0) or alkaline (such as pH 10.0) conditions. The characteristics of the bacteria have not been reported
At the same time, the common dosage form of Clostridium butyricum for feeding is Clostridium butyricum powder or liquid Clostridium butyricum dosage form. Clostridium butyricum has problems such as easy inactivation, heat resistance, and storage resistance.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: 50mg chitosan (50kd) is dissolved in 50mL 1% acetic acid solution, adjust pH to 4.0; Slowly add equal volume Clostridium butyricum liquid (100 million cfu / mL) under magnetic stirring; After mixing Add 25mL sodium tripolyphosphate solution (1mg / mL) dropwise to Clostridium butyricum; stir at room temperature for 30 minutes, the mixture of Clostridium butyricum presents a uniform blue-white color, that is, the shell of Clostridium butyricum has been formed Glycan nanoparticles. The encapsulation rate of Clostridium butyricum was 88.0%; under the condition of pH 5.5, the resistance was increased by 42% after warming at 60°C for 30 minutes; under the condition of pH 5.5, the activity of Clostridium butyricum was lost by 5% when stored at room temperature for 90 days; When the pH is 3.0, the release rate is 92% within 10 minutes.

Embodiment 2

[0026] Example 2: 1000mg chitosan (1800kd) was dissolved in 50mL 1% acetic acid solution, and the pH was adjusted to 6.0; an equal volume of Clostridium butyricum solution (100 million cfu / mL) was slowly added under magnetic stirring; after mixing evenly Add 250 mL of sodium tripolyphosphate solution (1 mg / mL) dropwise to the mixture of Clostridium butyricum; stir at room temperature for 30 minutes, the mixture of Clostridium butyricum appears blue-white with a small amount of flocculent precipitate, that is, the bag has formed. Chitosan nanoparticles embedded with Clostridium butyricum. The encapsulation rate of Clostridium butyricum was 83%; under the condition of pH 5.5, the resistance was increased by 50.2% after warming at 60°C for 30 minutes; under the condition of pH 5.5, stored at room temperature for 90 days, the activity of Clostridium butyricum was lost by 4.6%; When the pH is 10.0, the release rate within 10 minutes is 82.4%.

Embodiment 3

[0027] Embodiment 3: Dissolve 475mg chitosan (900kd) in 50mL 1% acetic acid solution, adjust pH to 5.0; Slowly add equal volume Clostridium butyricum liquid (100 million cfu / mL) under magnetic stirring; After mixing uniformly Add 120mL sodium tripolyphosphate solution (1mg / mL) dropwise to the mixture of Clostridium butyricum; stir at room temperature for 30 minutes, the mixture of Clostridium butyricum appears uniform blue-white, that is, the embedded Clostridium butyricum has formed chitosan nanoparticles. The encapsulation rate of Clostridium butyricum was 87.3%; under the condition of pH 5.5, the resistance was increased by 75% after warming at 60°C for 30 minutes; under the condition of pH 5.5, the liveness of Clostridium butyricum lost 2.7% when stored at room temperature for 90 days; When the pH is 7.0, the release rate is 31% within 10 minutes.

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Abstract

The invention relates to a preparation method of clostridium butyricum embedded chitosan nanoparticles in the technical field of feed additives. The preparation method comprises the following steps: preparing a chitosan stock solution; adding an isopyknic clostridium butyricum bacteria liquid into the chitosan stock solution, uniformly mixing by a magnetic stirring method to prepare a clostridium butyricum mixed liquor; and dropwisely adding a sodium tripolyphosphate aqueous solution into the clostridium butyricum mixed liquor, carrying out magnetic stirring at room temperature so as to prepare the clostridium butyricum embedded chitosan nanoparticles. The preparation method is simple to operate. The clostridium butyricum embedded chitosan nanoparticles can be obtained by simple mixing of chitosan / clostridium butyricum / sodium tripolyphosphate. The particles have advantages of no adhesion, good spheronization and round surface. Under an acid or alkali condition consistent with acid-base value of pig stomach and intestine, clostridium butyricum can be rapidly released, thus helping better perform efficacy of clostridium butyricum for feed.

Description

technical field [0001] The invention relates to a chitosan nanoparticle in the technical field of feed additives and a preparation method thereof, in particular to a chitosan nanoparticle embedding Clostridium butyricum and a preparation method thereof. Background technique [0002] CN101664111A, title "chitosan nanoparticles embedded with α-galactosidase and preparation method thereof", comprising: configuring chitosan stock solution; adding an equal volume of α-galactosidase crude enzyme to chitosan stock solution solution, mixed uniformly by magnetic stirring method to make α-galactosidase mixed solution; add sodium tripolyphosphate aqueous solution dropwise to α-galactosidase mixed solution, and magnetically stir at room temperature to make embedding Chitosan nanoparticles for alpha-galactosidase. The preparation and operation of the present invention are simple, and chitosan nanoparticles embedded with α-galactosidase can be obtained by simple mixing of chitosan / α-gala...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00
Inventor 王贤勇钱利纯许尧兴王裕舟费晓雯
Owner 杭州银冠海洋生物技术研究院有限公司
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