In-bottle production method of detoxified small seed ball of east lily
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A technology for lily and seed bulbs is applied in the field of lily bulb breeding to achieve the effects of improving quality, shortening cultivation period and reducing production costs
Inactive Publication Date: 2006-04-26
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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Problems solved by technology
In the production process of virus-free seedlings (balls), existing reports are all breeding and production with virus-free seedlings, and there is no report of directly generating virus-free small seed balls in the bottle
Method used
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Embodiment 1
[0052] 1), selection and sterilization of explants: the bulbs of the oriental lily variety "Tiber" with strong growth, good flowering traits, no pests and diseases, and no virus symptoms are selected as explants. Store the bulbs at a low temperature of 4°C in the dark for 8 weeks, then take them out, rinse them with tap water while peeling off the scales, remove the outer skin of the bulbs and the outer 2-3 layers of scales that are not tightly wrapped, and wash them with 0.1% washing powder water. Soak for 2 minutes, clean with tap water, cut off a little top and base, peel off into a single scale with a part of the base plate, in 0.1% mercuric chloride (Hgcl 2 ) solution for 25 minutes, sterilized in 1% sodium hypochlorite solution for 15 minutes, rinsed with sterile water for 3 times, and cut the scales into pieces for inoculation;
[0053] 2), induction culture: under sterile conditions, inoculate the sterilized scale cuts into the following induction medium:
[0054] MS ...
Embodiment 2
[0080] 1) The bulbs of the oriental lily variety "Acapulco" with strong growth, good flowering properties, no pests and diseases, and no virus symptoms are selected as explants. Take it out after 8 weeks of storage, rinse with tap water while peeling the scales, remove the outer skin of the bulb and the outer 2-3 layers of scales that are not tightly wrapped, soak in 0.1% washing powder water for 5 minutes, wash with tap water, and cut off a little top and the base, stripped into individual scales with part of the basal plate, in 0.2% mercury chloride (Hgcl 2 ) solution for 15 minutes, and 3% sodium hypochlorite solution for 10 minutes, after rinsing with sterile water for 5 times, the scales were cut into pieces for inoculation;
[0081] 2), induction culture: under sterile conditions, inoculate the sterilized scale cuts into the following induction medium:
[0082] MS Basic Medium
[0083] 6-Benzylaminopurine (6-BA) 2.0mg / L
[0084] Naphthaleneacetic acid (NAA) 0.5mg / L
...
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Abstract
The invention provides a process for producing the oriental lily sterilized seed globule in the bottle, comprising: choosing explant, sterilizing, inducing culture and getting the sterilized stem tip, then breeding culture, subculture, and producing the sterilized seed globule in the bottle directly. The invention produces the sterilized seed globule in the bottle directly to avoid the problem of long period of ball forming and the accumulation of virus existing in the oriental lily, the seed globule can be planted in the soil when out of the bottle, and the survival rate is high. The breeding procedure of the sterilized seed globule is simplified by modifying and optimizing the whole producing procedure for the lily seed globule, and the breeding time of sterilizing the oriental lily is shortened greatly, the quality of seed globule is improved when the producing cost is decreased, which reinforces market competitive power of the domestic self-breeding seed globule and is suitable for the mass production of the oriental lily sterilized seed globule.
Description
technical field [0001] The invention relates to a method for producing poison-free small seed bulbs of oriental lily in a culture bottle, and belongs to the technical field of lily bulb breeding. Background technique [0002] Lilium (Lilium) is a perennial herbaceous bulb plant of the genus Lilium in the family Liliaceae. It is a world-renowned ornamental flower because of its large flowers, beautiful color and elegant temperament. four. Oriental hybrids have the largest and most beautiful flowers among lily hybrids. The flowers are bowl-shaped or star-shaped, and can emit a pleasant fragrance, so they are also called perfumes. Lily, deeply loved by people, is a large class of lily hybrids widely cultivated in the world. In the cultivation process of lily bulbs, the production cycle of oriental lily bulbs is longer, and it takes 2.5 to 3 years to grow from scale cuttings to flowering commodity bulbs. At the same time, virus accumulation in conventional vegetative propagat...
Claims
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