58 results about "Glutamate synthetase" patented technology

The invention discloses a production method of a gamma-polyglutamic acid with high molecular weight, and belongs to the technical field of biological fermentation production of polymer materials. Bacillus licheniformis CGMCC NO.3336 is taken as an original strain, the rotating speed is reduced at a later stage of fermentation, the effect on polyglutamic acid synthesis caused by shearing force is reduced by regulating and controlling of ventilation and rotation speed in the fermentation process, and the temperature is changed, so as to affect the activity of folylpolyglutamate synthetase. Especially, the fermentation pH is controlled and the ion strength in the culture medium is increased by feeding glucose, sodium chloride, CaCl2.6H2O, MnSO4 and MgSO4, so as to obtain the polyglutamic acid with accurate molecular weight. The molecular weight is high, and the molecular weight of the gamma-polyglutamic acid is 1000000-4000000 Dalton by detection after fermentation is finished. The method is simple in technology, and the gamma-polyglutamic acid is high in molecular weight and relatively stable and controllable, and large-scale production can be achieved.

Sensitivity of a patient's cancer to treatment with 10-propargyl-10-deazaaminopterin is assessed and patients are selected for treatment of cancer with 10-propargyl-10-deazaaminopterin, by determining the amount of a selected polypeptide expressed by the cancer and comparing the amount with the amount of the selected polypeptide expressed by a reference cancer. The polypeptide includes a member of a folate pathway polypeptide within a cell and may include at least one of reduced folate carrier-1 enzyme (RFC-1), dihydrofolate reductase (DHFR), folylpoly-gamma-glutamate synthetase (FPGS), thymidylate synthase (TS), γ-glutamyl hydrolase (GGH), and glycinamide ribonucleotide formyltransferase (GARFT).

The invention relates to a special plant expression vector pH2-35S-PrbcS-GS1 which comprises an arabidopsis thaliana cytoplasm glutamine synthetase gene GS1 and can improve the utilization rate of a plant nitrogen element. A method of RT-PCR is used for cloning the GS1 gene from the arabidopsis thaliana of a model plant, a photoinduction type promotor (the promotor of a a small subunit Rubisco) is used for controlling the excessive expression of the GS1 gene in a plant leaf and a leaf disc conversion method is used for transferring the GS1 gene into a pPZP221-PrbcS-Dof1 type transgene tobacco. An experiment result shows that the GS1 gene can be normally transferred in the transgene tobacco; under the nutrition condition of low nitrogen and the growing conditions of indoor irradiation for 24 hours of 2000LUX and 25 DEG C, the growing situation of the plant transferred with the single gene of Dof1 is (the expression of the gene is controlled by the photoinduction type promotor Prbcs) is a little better than that of a contrast tobacco (a wild type without transgene); after being transferred under the natural growing condition of a green house, the growing situation of the tobacco which is simultaneously transferred with the GS1 gene and the Dof1 gene shows remarkable growing advantages than that of the contrast plant; and therefore, simultaneously and excessively expressing the GS1 gene and the Dof1 gene, can improve the efficiency of the GS / GOGAT (glutamine synthetase / glutamic acid synthetase) approaches in the leaf more extensively, thereby improving the utilization rate of the plant nitrogen element. The vector can be broadly applied to the molecule breeding of crops, improving the utilization rate of the plant nitrogen element thereof and the durability to the nutrition condition of low nitrogen and being capable of obtaining a higher yield under the conditions of applying less fertilizers and even not applying the fertilizers.

The invention discloses application of gamma-aminobutyric acid in improving salt stress resistant capacity of corn, relates to application of gamma-aminobutyric acid in growth and development of the corn, and aims at providing new application of gamma-aminobutyric acid. Gamma-aminobutyric acid has a regulating effect on oxidative damage and enzymatic activity of corn leaves under salt stress, the content of malonaldehyde in corn seedling leaves under the salt stress is decreased, the content of superoxide anions in the leaves is decreased, the content of superoxide dismutase in the leaves is decreased, improvement of peroxidase activity is promoted, the activity of an antioxidase system under NaCl stress is improved, the regulating effect on corn seedling nitrogen metabolism under the salt stress is achieved, the content of soluble protein of the corn leaves and root systems under the salt stress is increased, and pyruvic acid content, glutamine synthetase activity, glutamate synthase activity, glutamate dehydrogenase activity and glutamate decarboxylase activity in the corn leaves and the system roots under the salt stress are decreased. The application of gamma-aminobutyric acid in improving the salt stress resistant capacity of the corn is used in the field of corn cultivation.

Expression vectors are described that can efficiently produce virion capsid protein, tumor-associated protein of human papillomavirus on a microbial surface. Bacterial strains harboring such surface display vectors, and the use of the bacterial strains or their extracts or purified products as complex vaccines, are also described. The surface display vectors contain one or more than two genes selected from among pgsB, pgsC and pgsA, encoding a poly-χ-glutamic acid synthetase complex (pgsBCA) of a Bacillus sp. strain, and genes that encode virion capsid proteins, tumor-associated proteins of human papillomavirus, Methodology for preparing the foregoing vectors, vaccines and transformed microorganisms are also described.

The present invention relates to a surface expression vector having pgsBCA, a gene coding poly-gamma-glutamate synthetase and a method for expression of target protein at the surface of microorganism using the vector. The vector, in which foreign genes are inserted, transforms microorganisms and makes foreign proteins expressed stably on the surface of microorganisms.

The invention relates to the field of peanut cultivation techniques and in particular relates to a special growth regulator for peanuts in a fruiting period. The special growth regulator comprises 20-30g of paclobutrazol wettable powder, 2.5-3.0g of chitosan oligosaccharide, 50-100g of seaweed extract and 115-180g of saccharose. The content of chlorophyll of leaves, the leaf net photosynthetic rate and the root activity of the peanuts treated by the regulator in the fruiting period are obviously higher than corresponding contrasts, the leaf SOD (superoxide dismutase) activity and the POD (peroxidase) activity are both obviously higher than corresponding contrasts, the content of malondialdehyde is obviously lower than the contrast, and therefore, the regulator can be used for well eliminate oxygen free radicals and delayin g senescence. By utilizing the special growth regulator, the leaf nitrate reductase activity, the glutamine synthetase and glutamate synthase activities are obviously improved, the leaf sucrose synthetase and sucrose phosphate synthase activities are improved, and the accumulation of pod dry matters and compounds of nucleus protein and fat are facilitated. The peanut pod yield is obviously increased, the production increase reason is that the weight of single fruits is obviously increased, the full fruit rate is increased, and the double-kernel fruit rate and the kernel yield rate are greatly increased simultaneously.

The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Ana13 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Ana13 43 expressed on their surface, and the use thereof.According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention can be applied to other peptide antibiotics besides P5 3 and Ana13 35.

The present invention relates to a surface expression vector of SARS coronavirus antigen containing a gene encoding an antigen of SARS inducing coronavirus and any one or two or more of genes pgsB, pgsC and pgsA encoding poly-gamma-glutamic acid synthase complex, a microorganism transformed by the surface expression vector, and a SARS vaccine comprising the microorganism. According to the present invention, it is possible to economically produce a vaccine for prevention and treatment of SARS using a recombinant strain expressing an SARS coronavirus antigen on their surface.

The invention discloses a production method of a gamma-polyglutamic acid with high molecular weight, and belongs to the technical field of biological fermentation production of polymer materials. Bacillus licheniformis CGMCC NO.3336 is taken as an original strain, the rotating speed is reduced at a later stage of fermentation, the effect on polyglutamic acid synthesis caused by shearing force is reduced by regulating and controlling of ventilation and rotation speed in the fermentation process, and the temperature is changed, so as to affect the activity of folylpolyglutamate synthetase. Especially, the fermentation pH is controlled and the ion strength in the culture medium is increased by feeding glucose, sodium chloride, CaCl2.6H2O, MnSO4 and MgSO4, so as to obtain the polyglutamic acid with accurate molecular weight. The molecular weight is high, and the molecular weight of the gamma-polyglutamic acid is 1000000-4000000 Dalton by detection after fermentation is finished. The method is simple in technology, and the gamma-polyglutamic acid is high in molecular weight and relatively stable and controllable, and large-scale production can be achieved.

The invention discloses a high-efficiency production method of gamma-polyglutamic acid, and belongs to the technical field of production of polymer materials based on biological fermentation. According to the method, bacillus licheniformis CGMCC (China General Microbiological Culture Collection Center) NO.3336 for high-efficient production of gamma-polyglutamic acid is taken as a starting strain, sodium nitrate is added into a fermentation culture medium, and the activity of polyglutamate synthase is influenced under the nitrate respiration condition by adjusting and controlling the pH value and the ventilation quantity in a fermentation process, so that residual glutamic acid in a fermentation solution is reduced and the conversion rate of glutamic acid is increased; after fermentation, the glutamic acid content of the fermentation solution is 5-15 g / L and the gamma-polyglutamic acid yield is 45-55 g / L. Therefore, the economic and feasible production method is provided for industrialization production of gamma-polyglutamic acid.

The present invention relates to a method for assessing the sensitivity of a patient's cancer to treatment with 10-propargyl-10-deazaminopterin and a method for selecting a patient for treatment of cancer with 10-propargyl-10-deazaminopterin, by determining the amount of a selected polypeptide expressed by the cancer and comparing the amount with the amount of the selected polypeptide expressed by a reference cancer, wherein the polypeptide includes a member of folate pathways within cells and may include at least one of reduced folate carrier-1 enzyme (RFC-1), dihydrofolate reductase (DHFR), folylpoly-gamma-glutamate synthetase (FPGS), thymidylate synthase (TS), γ-glutamyl hydrolase (GGH), and glycinamide ribonucleotide formyltransferase (GARFT). The present invention also relates to the use of 10-propargyl-10-deazaminopterin in the treatment of multiple myeloma.