A Saccharomyces cerevisiae gene expression system and its construction and application

A Saccharomyces cerevisiae, gene expression technology, applied in the field of genetic engineering, can solve problems such as low protein expression, low plasmid copy number, and inability to meet large-scale production
CN104630258BActive Publication Date: 2018-08-14JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2018-08-14

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Abstract

The invention relates to an integrated Saccharomyces cerevisiae gene expression system which comprises an expression vector, wherein the expression vector sequentially comprises the following operable elements from 5'-3': a pMD19-Tsimple plasmid framework, an rDNA homologous recombinant sequence, an exogenous gene expression cassette and a selective marker gene expression cassette; the exogenous gene expression cassette sequentially comprises a promoter, an exogenous gene insertion enzyme digestion site and a transcription terminator from upstream to downstream; and the selective marker gene expression cassette comprises a promoter, an antibiotic resistance gene and a transcription terminator. The yeast is Saccharomyces cerevisiae. The expression vector can implement integrated stable expression in the Saccharomyces cerevisiae, and has important meanings for fundamental research and product development of the Saccharomyces cerevisiae.
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Description

technical field

[0001] The invention relates to the technical field of genetic engineering, in particular to an integrated Saccharomyces cerevisiae gene expression system and its construction method and application. Background technique

[0002] With the rapid development of genomics, people are urgently seeking suitable expression systems for the mining of new genes and the construction of new engineered cells. For this reason, various expression systems have emerged, such as bacteria, insect cells, yeast, and mammalian cells. Animal cell expression system, etc. In recent years, the yeast expression system represented by Saccharomyces cerevisiae has become an important tool for metabolic engineering to produce bio-based products, effectively express new foreign genes, and serve basic research, industrial and medical applications due to its unique biological characteristics. Saccharomyces cerevisiae is the yeast that has been produced, lived the longest, and is most closely...

Claims

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