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Immunoglobulin A nephropathy T cell diagnostic marker

A technology of markers and nephropathy, applied in the field of nephropathy detection, can solve the problems of low specificity of immunodiagnostic markers, failure of early diagnosis by renal puncture, and inability of patients to obtain a definite diagnosis, etc.

Pending Publication Date: 2022-04-12
SHENZHEN LUWEI BIOTECHNOLOGY (BIOMANIFOLD TECH CO) LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, the gold standard for the diagnosis of IgA nephropathy is pathological tissue biopsy of renal puncture. However, invasive renal puncture has several disadvantages: (1) Renal puncture cannot be used for early diagnosis and can only detect patients with established renal damage
(2) Renal puncture is risky, because many patients have relative contraindications for renal puncture, or the hospital does not have the conditions for pathological diagnosis of renal puncture, so patients cannot obtain a clear diagnosis and receive targeted treatment
(3) The medical cost of renal puncture is high, which is equivalent to one operation and needs to be hospitalized for one week
However, the specificity of these immunodiagnostic markers is not high, therefore, it is necessary to find more diagnostic markers through new methods

Method used

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  • Immunoglobulin A nephropathy T cell diagnostic marker
  • Immunoglobulin A nephropathy T cell diagnostic marker
  • Immunoglobulin A nephropathy T cell diagnostic marker

Examples

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Effect test

Embodiment 1

[0054] Example 1: Screening of markers

[0055] The examples of this application relate to the screening of diagnostic markers. Previous studies have shown that mRNA gene expression has great potential for molecular diagnosis of kidney disease, and the pathogenesis of immunoglobulin A nephropathy may be related to the T Cell Immunity Axis. Some gene axes (Axis) are related. Therefore, this protocol utilizes T cell-specific gene or protein expression to screen for potential diagnostic markers.

[0056] Preselected genes associated with five T cell classes that may be involved in the pathogenesis of immunoglobulin A nephropathy are as follows:

[0057] 1. Helper T Cell 1 Th1-related genes: CCL4, CCR2, CCR5, CXCR3, IFNG, IFNGR1, IFNGR2, IL2, IL20RA, JAK2, JAK3, STAT1, STAT4, TBR1, TNF;

[0058] 2. Helper T Cell 2 Th2-related genes: BCL2, BCL2L1, CCR3, CCR4, CCR8, CD28, CXCL11, CXCL12, GATA3, IL10, IL13, IL33, IL4, IL4R, IL5, NFATC2IP, STAT6;

[0059] 3. Helper T Cell 17 Th17-...

Embodiment 2

[0085] This embodiment provides a device for IgA nephropathy risk assessment, the device includes a processor and a memory, and the memory stores a computer program that can be executed by the processor. The method of using this device to assess the risk of IgA nephropathy in subjects is as follows:

[0086] 1. Select subjects' peripheral blood samples to extract exosomal mRNA.

[0087] 2. Send the extracted mRNA into a detection device (eg, a standard qPCR platform) for quantitative data on the expression of the seven genetic diagnostic markers provided in Example 1: CD4, CD8A, CCR3, GATA3, GZMA, RORA, VEGFC.

[0088] 3. Use the device to retrain a linear regression model using clinical observations as target variables (eg, proteinuria, eGFR, pathological grade by renal biopsy, 5- or 10-year uremia risk, drug efficacy prediction, drug resistance) , and determine the parameter vector w for peripheral blood samples according to the optimal linear regression model obtained n (...

Embodiment 3

[0090] This example provides a kit, including reagents capable of quantifying the mRNA levels of CD4, CD8A, CCR3, GATA3, GZMA, HDAC7, RORA and VEGFC, the reagents include reverse transcriptase, primers, Taq enzyme, fluorescent dyes, and the like.

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Abstract

The invention discloses an immunoglobulin A nephropathy T cell diagnostic marker. On the first aspect, the invention provides application of a reagent for quantitatively detecting at least one of the following markers in preparation of the diagnostic kit for IgA nephropathy: CCR3, CD4, CD8A, GATA3, GZMA, HDAC7, RORA and VEGFC. According to the application disclosed by the embodiment of the invention, the application at least has the following beneficial effects that the pathogenesis of the immunoglobulin A nephropathy is related to five gene axes (Axis), screening is carried out from related mRNA gene expression data based on a specific gene or protein of a T cell from the T cell immune axis (T Cell Immunity Axis) to obtain the eight markers, and the immunoglobulin A nephropathy can be used for detecting the immunoglobulin A nephropathy. Whether a subject suffers from IgA nephropathy or not can be efficiently and accurately diagnosed through quantitative detection on the basis of at least one of the eight markers, and good specificity and sensitivity are achieved.

Description

technical field [0001] The present application relates to the technical field of nephropathy detection, in particular to immunoglobulin A nephropathy T cell diagnostic markers. Background technique [0002] Immunoglobulin A (IgA) nephropathy, which is caused by the deposition of IgA complexes in the kidneys, leads to local autoimmune reactions in the kidneys, and causes renal tissue lesions. It is the most common primary glomerular disease. More than 30% of patients progress to end-stage renal disease (ESRD) 10-20 years after onset, making IgA nephropathy one of the most common causes of uremia. At present, the gold standard for the diagnosis of IgA nephropathy is pathological biopsy of renal biopsy. However, invasive renal biopsy has several defects: (1) Renal biopsy cannot be used for early diagnosis, and it can only detect patients with established renal damage. (2) Renal puncture is risky, because many patients have relative contraindications to renal puncture, or the h...

Claims

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Application Information

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IPC IPC(8): G01N33/68G16B40/00
CPCC12M1/00C12N15/09C12Q1/68C12Q1/6886G01N33/48G01N33/68G16B40/00
Inventor 饶皑炳
Owner SHENZHEN LUWEI BIOTECHNOLOGY (BIOMANIFOLD TECH CO) LTD
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