Shrna sequence and application thereof for inhibiting expression of mouse macf1 gene

A gene expression and mouse technology, applied in the field of shRNA sequence, can solve the problem of low efficiency and achieve the effect of improving efficiency
CN104531700BActive Publication Date: 2017-12-29NORTHWESTERN POLYTECHNICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NORTHWESTERN POLYTECHNICAL UNIV
Publication Date
2017-12-29

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Abstract

The invention discloses an shRNA sequence for inhibiting mouse MACF1 gene expression and application thereof, which is used to solve the technical problem of low efficiency of the existing shRNA sequence for inhibiting mouse MACF1 gene expression. The technical solution is to clone the shRNA sequence that inhibits the expression of the mouse MACF1 gene into the pGLV3 lentiviral vector to obtain a recombinant lentiviral vector containing the shRNA sequence; the obtained recombinant vector is used to transfect mouse cells to achieve inhibition Purpose of MACF1 gene expression in cells. The shRNA sequence mentioned above can obviously inhibit the expression of MACF1 gene at mRNA and protein levels in mouse preosteoblasts. The proliferation, migration and differentiation functions of preosteoblasts were inhibited by inhibiting the expression of MACF1 in mouse preosteoblasts. The inhibition rate of the present invention at the protein level reaches over 90%, and the inhibition rate at the gene level reaches 81%.
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Description

technical field

[0001] The invention relates to an shRNA sequence, in particular to an shRNA sequence for inhibiting mouse MACF1 gene expression. It also relates to the application of this shRNA sequence for suppressing mouse MACF1 gene expression. Background technique

[0002] Microtubule actin crosslinking factor 1 (MACF1), also known as ACF7 (Actin crosslinking factor 7), is a new cytoskeleton crosslinking molecule, located on 1p31-32, containing 5380 amino acids , by regulating microfilaments and microtubule skeleton dynamics, it plays an important role in regulating various biological functions such as cell migration, cell polarization, cell adhesion, cell signal transduction, and protein transport. Due to the wide distribution of MACF1 and its important role in various biological functions, it is of great significance to study its function in different tissues.

[0003] RNA interference (RNA interference, RNAi) refers to the phenomenon of efficient and specific degra...

Claims

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