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Preparation method and application of composite hydrogel, composite hydrogel repair material and preparation method thereof

A technology of composite hydrogel and repair material, which is applied in the field of composite hydrogel repair material and its preparation, and the preparation field of composite hydrogel, and can solve the problem that the performance needs to be improved, etc.

Inactive Publication Date: 2019-03-22
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Boston University in the United States has recently researched that cartilage is soaked in two kinds of polymer monomers and initiators, and under light, a polymer network structure material that can repair damaged cartilage, that is, hydrogel, is formed. This material can indeed repair the degeneration that has occurred. Cartilage with sexual lesions plays a strengthening role ("Progress in Biomedical Engineering" 2016,37,1), but its performance still needs to be improved

Method used

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  • Preparation method and application of composite hydrogel, composite hydrogel repair material and preparation method thereof
  • Preparation method and application of composite hydrogel, composite hydrogel repair material and preparation method thereof
  • Preparation method and application of composite hydrogel, composite hydrogel repair material and preparation method thereof

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preparation example Construction

[0028] The invention provides a method for preparing a composite hydrogel, comprising: S1) reacting chondroitin sulfate and a first acrylate monomer in a phosphate buffer to obtain a chondroitin sulfate acrylate hydrogel monomer ; After the chondroitin sulfate acrylate hydrogel monomer is oxidized by sodium periodate, the oxidized chondroitin sulfate acrylate hydrogel monomer is obtained;

[0029] S2) Under the condition of ultraviolet light, the oxidized chondroitin sulfate acrylate hydrogel monomer and the degradable hydrogel monomer are polymerized by a photoinitiator to obtain a composite hydrogel.

[0030] The present invention has no special limitation on the sources of all raw materials, which can be commercially available.

[0031] Reaction of chondroitin sulfate and the first acrylate monomer in phosphate buffer; wherein, the first acrylate monomer is an acrylate monomer well known to those skilled in the art, and there is no special Restrictions, in the present inve...

Embodiment 1

[0062] Weigh 2g of chondroitin sulfate and put it into 50mL of phosphate buffered saline solution, and carry out magnetic stirring until it is completely dissolved (transparent). Use a pipette to pipette 15.0 mL of methacrylate (MA) and add it dropwise to the above solution. After adding MA dropwise for about 30 minutes, use 5M NaOH solution to adjust the solution to pH = 8.0. After the solution was placed at room temperature with magnetic stirring for 2 hours, it was placed in a refrigerator for 24 hours. Use 200mL of absolute ethanol to precipitate CS-MA, and keep stirring and mixing, wash the CS-MA precipitate with a large amount of absolute ethanol to remove unreacted MA, and then centrifuge. The sample was frozen at -80°C for 3 days to obtain the chondroitin sulfate acrylate hydrogel monomer, and the centrifuge tube was sealed with filter paper and a rubber band, and stored in a desiccator.

Embodiment 2

[0064]Weigh 5g of gelatin into 50mL of PBS solution, and carry out magnetic stirring until completely dissolved (transparent). Use a pipette gun to pipette 2.4mL of methacrylate (MA) and add it dropwise to the above solution. After the dropwise addition of MA, continue the magnetic stirring reaction in a water bath with a constant temperature of 50°C for 3h, and use 200ml of PBS at 40°C for the reaction solution to terminate the reaction, and keep stirring to mix. The above solution was put into a dialysis bag, and dialyzed in pure water at 40° C. for 6 days with constant stirring. The dialyzed solution was centrifuged and frozen at -80°C for 3 days to obtain a gelatin acrylate hydrogel monomer (GelMA hydrogel monomer), which was stored in a desiccator.

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Abstract

The invention provides a preparation method of a composite hydrogel. The preparation method comprises the following steps: S1) carrying out a reaction between chondroitin sulfate and a first acrylate monomer in a phosphate buffer solution to obtain a chondroitin sulfate acrylate hydrogel monomer; oxidizing the chondroitin sulfate acrylate hydrogel monomer to obtain an oxidized chondroitin sulfate acrylate hydrogel monomer; S2) under an ultraviolet radiation condition, carrying out a polymerization reaction between the oxidized chondroitin sulfate acrylate hydrogel monomer and a degradable hydrogel monomer through initiation of a photoinitiator so as to obtain the composite hydrogel. Compared with the prior art, a Schiff's base reaction of aldehyde groups contained in the oxidized chondroitin sulfate acrylate hydrogel monomer and amino groups existing in a tissue can be carried out, so that the adhesion of the tissue inside the body is more facilitated; moreover, the composite hydrogel contains normal cartilage tissue components, a novel cartilage tissue is easy to form quickly, and the composite hydrogel has potential application values in the aspect of clinical cartilage repair.

Description

technical field [0001] The invention belongs to the technical field of medical material manufacturing, and in particular relates to a preparation method and application of a composite hydrogel, a composite hydrogel repair material and a preparation method thereof. Background technique [0002] Articular cartilage is a special connective tissue mainly composed of collagen, proteoglycan and water. However, because there are no blood vessels, nerves, and lymphoid tissues, once the articular cartilage is damaged, it has almost no effective self-repair and reconstruction capabilities. Artificial joint replacement and articular cartilage tissue engineering are two important methods to repair articular cartilage damage and restore joint function. Some foreign scholars proposed a new type of artificial joint design with "soft cushion bearing" based on artificial joint replacement. In China, Zou Jun et al. also proposed to build a new type of artificial joint with soft cushion beari...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/28C08J3/24C08J3/075C08L89/00C08L5/08C08B37/08C08H1/00A61L27/20A61L27/16A61L27/24A61L27/52A61L27/56
CPCA61L27/16A61L27/20A61L27/24A61L27/52A61L27/56A61L2300/232A61L2430/06C08B37/0069C08H1/00C08J3/075C08J3/246C08J3/28C08J2389/00C08J2405/08C08L89/00C08L2201/06C08L2203/02C08L5/08C08L33/08C08L5/04
Inventor 刘燕谭帼馨周蕾
Owner GUANGDONG UNIV OF TECH
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