Preparation of coaxial electrospun-containing multilayered cartilage complex by electrospinning 3D printing

A coaxial electrospinning and 3D printing technology, applied in the field of 3D printing, can solve the problems that normal cartilage cannot provide friction resistance and support, and is easy to be damaged again, so as to promote cell proliferation and differentiation, improve printing accuracy, and high structural accuracy Effect
CN109701079AInactive Publication Date: 2019-05-03SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
Publication Date
2019-05-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention belongs to the technical field of 3D printing and particularly discloses a preparation of a coaxial electrospun-containing multilayered cartilage complex by electrospinning 3D printing. The preparation comprises the following steps: S1, seed cell culturing; S2, three-phase integrated scaffold preparing; and S3, cell planting. The method uses the coaxial electrospinning 3D printing technology, can accurately control fiber diameter and printing path, and greatly improves printing precision compared with ordinary spray-type printing, diameter of monofilament can be as thin as 10 [mu]m, and besides, the coaxial spinning technology can obtain double-layer fibers containing cytokines in an inner layer, conducts layer-induction of corresponding seed cells to differentiate into cells in corresponding layers on the scaffold, and finally forms a layered structure similar to normal cartilage tissues. The complex obtained by the method has a layered structure similar to normal articular cartilage tissues and has high structural precision, and the loaded cytokines are favorable for promoting cell proliferation and differentiation, and beneficial to repair of cartilage damages.
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Description

technical field

[0001] The invention belongs to the technical field of 3D printing, and in particular relates to electrospinning 3D printing to prepare a multilayer cartilage complex containing coaxial electrospinning. Background technique

[0002] The damage and degeneration of articular cartilage is a very common form of disease, and it is also one of the main problems faced in orthopedic clinical work. The incidence of various diseases caused by articular cartilage damage and degeneration is also increasing year by year. Therefore, how to intervene or induce the repair of cartilage damage has become the focus of current research. However, simply inducing the generation of chondrocytes at the injury site cannot solve the key problem, the main reason being that the new cartilage cannot meet the functional requirements of normal articular cartilage. The structure of articular cartilage can be mainly divided into four layers: superficial layer, middle layer, deep layer and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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