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Preparation of coaxial electrospun-containing multilayered cartilage complex by electrospinning 3D printing

A coaxial electrospinning and 3D printing technology, applied in the field of 3D printing, can solve the problems that normal cartilage cannot provide friction resistance and support, and is easy to be damaged again, so as to promote cell proliferation and differentiation, improve printing accuracy, and high structural accuracy Effect

Inactive Publication Date: 2019-05-03
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in this method, the generated cartilage does not have the layered structure of normal cartilage. Therefore, it cannot provide the friction resistance and support function of normal cartilage, and it is easy to be damaged again and cause symptoms. Therefore, in addition to improving surgical skills and rehabilitation programs In addition, an effective method is needed to reconstruct and repair the normal structure and function of cartilage

Method used

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  • Preparation of coaxial electrospun-containing multilayered cartilage complex by electrospinning 3D printing
  • Preparation of coaxial electrospun-containing multilayered cartilage complex by electrospinning 3D printing
  • Preparation of coaxial electrospun-containing multilayered cartilage complex by electrospinning 3D printing

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Effect test

preparation example Construction

[0033] S2, the preparation of three-phase integrated support;

[0034] S3, planting of cells, taking an appropriate amount of bone marrow mesenchymal stem cells to plant in each scaffold area.

[0035] Specifically, the method for culturing the seed cells in step S1 is that the bone marrow mesenchymal stem cells are cultured in a DMEM medium containing 10% fetal bovine serum in an incubator.

[0036] Specifically, the temperature in the incubator is 37°C, and there is 5% CO in the incubator 2 .

[0037] Specifically, the establishment of the model in the preparation step of the three-phase integrated stent in the step S2 includes the following steps;

[0038] S21, establishment of the model;

[0039] S22, preparation of materials;

[0040] S23. Equipment preparation.

[0041] Specifically, the specific process of establishing the model in the step S21 is: use three-dimensional printing software to establish a printing model, which is in the shape of a multi-layer cylinder...

Embodiment 1

[0047] (1) Use 3D printing software to build a printing model, which is multi-cylindrical, with different path gaps in the surface area, middle-deep area, and calcification layer area, and save it;

[0048](2) Measure an appropriate amount of polymer materials polycaprolactone and polylactic acid, and the polylactic acid is mixed with appropriate amounts of TGFβ1, BMP7, IGF cytokines and HA (hydroxyapatite) particles;

[0049] (3) Equipment preparation: adjust the process parameters of the electrospinning 3D printing equipment: the diameter of the printing wire is 10-50 μm, the printing temperature is 65°C, the air pressure of the barrel is 600-1000KPa during the printing process, and the voltage of the negative high voltage module is - 2.5~-50kV, the printing structure is controlled by the printing path, the printing path is 0 / 90°, the printing interval is 100μm, 150μm, 200μm, the polycaprolactone is loaded into the outer barrel, and the polylactic acid solution loaded with cy...

Embodiment 3

[0051] The biocompatibility of the obtained cartilage layered scaffold was characterized, and the obtained coaxial mixed cytokine three-phase integrated scaffold was planted with bone marrow stem cells and cultured in an incubator. The scaffold obtained by three-dimensional printing of pure polycaprolactone and bone marrow stem cells were used as the control group, and compared with each area of ​​the three-phase scaffold. After cultured for 1, 3, 5, and 7 days, the cell viability in the surface area scaffold was detected by MTT method, which showed that the cells on the scaffold obtained by electrospinning 3D printing technology with cytokine microspheres had good proliferation behavior, and the cells The vitality is better, and the proliferation behavior is more significant than that of the control group.

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Abstract

The present invention belongs to the technical field of 3D printing and particularly discloses a preparation of a coaxial electrospun-containing multilayered cartilage complex by electrospinning 3D printing. The preparation comprises the following steps: S1, seed cell culturing; S2, three-phase integrated scaffold preparing; and S3, cell planting. The method uses the coaxial electrospinning 3D printing technology, can accurately control fiber diameter and printing path, and greatly improves printing precision compared with ordinary spray-type printing, diameter of monofilament can be as thin as 10 [mu]m, and besides, the coaxial spinning technology can obtain double-layer fibers containing cytokines in an inner layer, conducts layer-induction of corresponding seed cells to differentiate into cells in corresponding layers on the scaffold, and finally forms a layered structure similar to normal cartilage tissues. The complex obtained by the method has a layered structure similar to normal articular cartilage tissues and has high structural precision, and the loaded cytokines are favorable for promoting cell proliferation and differentiation, and beneficial to repair of cartilage damages.

Description

technical field [0001] The invention belongs to the technical field of 3D printing, and in particular relates to electrospinning 3D printing to prepare a multilayer cartilage complex containing coaxial electrospinning. Background technique [0002] The damage and degeneration of articular cartilage is a very common form of disease, and it is also one of the main problems faced in orthopedic clinical work. The incidence of various diseases caused by articular cartilage damage and degeneration is also increasing year by year. Therefore, how to intervene or induce the repair of cartilage damage has become the focus of current research. However, simply inducing the generation of chondrocytes at the injury site cannot solve the key problem, the main reason being that the new cartilage cannot meet the functional requirements of normal articular cartilage. The structure of articular cartilage can be mainly divided into four layers: superficial layer, middle layer, deep layer and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/54
Inventor 乔之光韩煜戴尅戎孙彬彬连梅菲唐佳昕
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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