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A kind of bionic cartilage based on 3D printing and its manufacturing method

A 3D printing and cartilage technology, applied in the field of biomedical engineering, can solve problems such as difficult to prepare, achieve the effect of promoting healing, overcoming poor repair effect, good mechanical properties and vascularization ability

Active Publication Date: 2020-08-04
GUANGDONG TAIBAO MEDICAL DEVICE TECH RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cartilage tissue engineering provides an ideal therapeutic approach for the treatment of cartilage injuries, but it is difficult to prepare a high-quality cartilage repair material with a physiological structure and physiological properties similar to normal cartilage tissue, and there are many problems to be solved , such as the fixation of new cartilage tissue, immune rejection after allogeneic cell transplantation, selection of seed cells and growth factors, how to solve the long-term degeneration of cultured cartilage, whether it can withstand long-term load and friction, etc.

Method used

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  • A kind of bionic cartilage based on 3D printing and its manufacturing method
  • A kind of bionic cartilage based on 3D printing and its manufacturing method
  • A kind of bionic cartilage based on 3D printing and its manufacturing method

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preparation example Construction

[0036] (1) Preparation of printing materials:

[0037] ① Preparation of modified type II collagen: weigh a certain amount of type II collagen (molecular weight: 1×10 6 kDa) was added to PBS phosphate at 50°C to prepare a solution with a mass concentration of 10%, and then methacrylic anhydride was added according to the volume ratio of type VII collagen PBS phosphate solution: V methacrylic anhydride = 10:0.75, in Stir the reaction at 50°C for 4 hours. After the reaction is over, place the solution obtained from the reaction in a dialysis bag with a molecular weight cut-off of 10,000 for dialysis for 7 days, and then place the obtained dialysate in a freeze dryer at -80°C for 48 hours. , the modified type II collagen was obtained, stored at room temperature, and set aside;

[0038] ② Preparation of modified hyaluronic acid: weigh a certain amount of hyaluronic acid (molecular weight 1.2~1.5×10 6 kDa) was dissolved in distilled water to prepare a hyaluronic acid solution with...

Embodiment 1

[0050] According to the present invention, a bionic cartilage based on 3D printing is prepared according to the above method, wherein: the molecular weight of type II collagen used in the preparation of the printing material is 1×10 6 kDa, the molecular weight of the hyaluronic acid used is 1.2×10 6 kDa; the particle size distribution range of nano-hydroxyapatite used in the preparation of each biomimetic layer precursor is 150-250 nm; the amount of nano-hydroxyapatite used in the preparation step of biomimetic subchondral bone precursor is 5 parts by mass, the amount of modified type II collagen is 5 parts by mass, the amount of photoinitiator Irgacure2959 is 0.5 parts by mass; the amount of modified hyaluronic acid in the preparation step of the biomimetic calcification layer precursor is 30 parts by mass, The amount of apatite used is 5 parts by mass, and the amount of photoinitiator Irgacure2959 is 0.5 parts by mass; the amount of modified hyaluronic acid used in the prepa...

Embodiment 2

[0052] According to the present invention, a bionic cartilage based on 3D printing is prepared according to the above method, wherein: the molecular weight of type II collagen used in the preparation of the printing material is 1×10 6 kDa, the molecular weight of the hyaluronic acid used is 1.2×10 6 kDa; the particle size distribution of nano-hydroxyapatite used in the preparation of each biomimetic layer precursor is 150-250 nm; the amount of nano-hydroxyapatite in the preparation step of biomimetic subchondral bone precursor is 10 mass parts, the amount of modified type II collagen is 10 parts by mass, the amount of photoinitiator Irgacure2959 is 0.5 parts by mass; The amount of limestone used is 5 parts by mass, the amount of photoinitiator Irgacure2959 is 0.5 parts by mass; the amount of modified hyaluronic acid used in the preparation step of biomimetic cartilage deep layer precursor is 15 parts by mass, and the amount of modified type II collagen is 10 parts by mass, th...

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Abstract

The invention belongs to the technical field of biomedical engineering, especially the technical field of bionic material preparation, and discloses a bionic cartilage based on 3D printing and a manufacturing method thereof, including a bionic cartilage based on 3D printing and a 3D printed bionic cartilage Manufacturing method. The 3D printing-based biomimetic cartilage is a multi-layer structure, which is prepared by gradient biomimetic 3D printing from modified type II collagen, modified hyaluronic acid, nano-hydroxyapatite and chondrocytes. The bionic cartilage has good biocompatibility and mechanical properties equivalent to normal cartilage tissue, and is beneficial to the repair of cartilage defect parts.

Description

technical field [0001] The invention belongs to the technical field of biomedical engineering, in particular to a bionic cartilage based on 3D printing and a manufacturing method thereof. Background technique [0002] Cartilage degeneration or damage caused by trauma or disease is relatively common in clinical practice. However, due to the special nature of articular cartilage tissue, there are no blood vessels, lymphatic vessels and nerves inside, and there is only very low or almost no effective self-repair ability after traumatic injury. If the treatment is not timely or appropriate, it will lead to serious functional impairment. Like other tissue repair processes, cartilage repair occurs through the formation of a fibrin clot followed by a cascade of chondroblasts derived from normal cartilage surrounding the defect, bone marrow beneath the defect, or synovial tissue, ultimately forming the damaged site repair tissue. Several existing cartilage repair techniques, such ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/46A61L27/44A61L27/38B33Y80/00
Inventor 韦加娜卢亢陈泰瀛
Owner GUANGDONG TAIBAO MEDICAL DEVICE TECH RES INST CO LTD
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