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Articular cartilage repairing material based on oxidized hyaluronic acid-type II collagen and autologous concentration bone marrow nucleated cells and preparation method

A technology for oxidizing hyaluronic acid and bone marrow nucleated cells, which is applied in the field of medical materials, can solve the problems of poor repair tissue mechanical properties, poor long-term curative effect, fibrocartilage degeneration, etc., and achieve good repair effect

Active Publication Date: 2018-06-01
重庆市人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used repair strategies for damaged cartilage in clinic mainly include: joint cavity cleaning, microfracture, cartilage transplantation, chondrocyte transplantation, osteochondral transplantation, etc.
Cartilage tissue engineering technology is an ideal method for the repair of articular cartilage damage, but there are still many problems in the use of tissue engineering technology for cartilage repair at this stage, such as: more complicated procedures; poor mechanical properties of repaired tissues, poor integration with host tissues, and fibrous Cartilage degeneration, etc.

Method used

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  • Articular cartilage repairing material based on oxidized hyaluronic acid-type II collagen and autologous concentration bone marrow nucleated cells and preparation method
  • Articular cartilage repairing material based on oxidized hyaluronic acid-type II collagen and autologous concentration bone marrow nucleated cells and preparation method
  • Articular cartilage repairing material based on oxidized hyaluronic acid-type II collagen and autologous concentration bone marrow nucleated cells and preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1, Preparation of Oxidized Hyaluronic Acid Type II Collagen Biomimetic Gel

[0028] (1) Extraction and identification of cartilage type II collagen hydrogel

[0029] a. Preparation of cartilage powder: obtain the hyaline cartilage of the knee joint of fresh Guizhou Xiaoxiang pig, freeze it in a -20°C refrigerator for 12 hours, collect the frozen cartilage in a glass dish, place it in a vacuum freeze dryer, and freeze-dry it after 24 hours (the temperature drops to -66°C, pressure down to 1Pa), after freeze-drying, pour the cartilage slices into a liquid nitrogen low-temperature pulverizer, continuously fill with liquid nitrogen, crush the cartilage slices into dry cartilage powder, collect the cartilage powder and store it in a sealed bag for later use;

[0030] b. Extraction of Type II Collagen: Weigh 20g of freeze-dried cartilage powder and place it in a beaker, pour 1L of 4M guanidine hydrochloride solution into the suspension, stir continuously with a magnet...

Embodiment 2

[0049] Example 2. Experiment of repairing cartilage defect with biomimetic gel combined with autologous enriched bone marrow nucleated cells

[0050] In order to explore the effect of biomimetic gel combined with autologous concentrated bone marrow nucleated cells in repairing articular cartilage damage, an animal experiment was designed to repair cartilage defects with a one-step method. The bionic gel combined with autologous concentrated bone marrow nucleated cells was used to repair pig knee articular cartilage defects. Taking Guizhou Xiaoxiang pigs as experimental animals, the bone marrow was first aspirated at the iliac crest under general anesthesia, and the autologous concentrated bone marrow nucleated cells were obtained through separation of bone marrow separation fluid, mixed with type II collagen, and then added with oxidized hyaluronic acid to cross-link. A full-thickness cartilage defect model with a diameter of 8 mm was prepared at the femoral trochlea of ​​the ...

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Abstract

The invention relates to an articular cartilage repairing material based on oxidized hyaluronic acid-type II collagen and autologous concentration bone marrow nucleated cells and a preparation method.The articular cartilage repairing material is prepared from oxidized hyaluronic acid, type II collagen and autologous concentration bone marrow nucleated cells; the autologous concentration bone marrow nucleated cells in the material have potential in self-renewal and chondrogenic differentiation, participate in cartilage defect repairing, and thus realize a perfect cartilage defect repairing effect; the repaired tissue appears like hyaline cartilage and is obviously superior to the blank control group in surface flatness, integration degree with adjacent normal cartilage, content of type IIcollagen, GAG content, forms of calcified cartilage and subchondral bone and the like, and has potential in clinical transformation.

Description

technical field [0001] The invention belongs to the field of medical materials, and relates to an articular cartilage repair material based on oxidized hyaluronic acid-II collagen and autologous concentrated bone marrow nucleated cells, and also relates to a preparation method and application of the material. Background technique [0002] Articular cartilage is a thin layer of dense connective tissue lining the surface of joints. Its surface is smooth, firm and elastic, and it is an important load-bearing tissue for human joints. However, since normal articular cartilage has no blood vessels, nerves, and lymphatic distribution, it is difficult to repair itself after injury, and there is still a lack of effective repair strategies. In clinical work, the incidence of articular cartilage damage is relatively high. Widuchowski retrospectively analyzed 25,124 cases of arthroscopic knee surgery and found that 60% of the cases had cartilage damage. Cartilage damage has become an i...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/24A61L27/38C08B37/08C12P21/06C07K1/14C07K1/30C12N5/077
CPCA61L27/20A61L27/24A61L27/38A61L27/3852A61L2430/06C07K14/78C08B37/0072C12N5/0669C12N2509/00C12P21/06C08L5/08C08L89/00
Inventor 刘俊利曾伟南曾一平谭祖键周明全贾小林张胜利郝玉徽
Owner 重庆市人民医院
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