Grafted protein hydrogel biological material and its preparing process
A technology of biomaterials and hydrogels, applied in medical science, prostheses, coatings, etc., can solve problems such as unsuitable cell adhesion, and achieve the effects of promoting wound healing, regulating inflammation, and inhibiting scar tissue formation
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Embodiment 1
[0015] Example 1 Preparation of Hyaluronic Acid Hydrogel
[0016] Prepare a hyaluronic acid solution with a mass percentage of 1% with deionized water, add 1M hydrochloric acid solution dropwise to the hyaluronic acid solution, and stir at the same time to fully mix the two until the pH value reaches 4.75; Weigh adipic dihydrazide and add it into the hyaluronic acid solution at 10% to 25% of the hyaluronic acid, stir well; then weigh carbodiimide hydrochloride and add it to the hyaluronic acid solution, stir for 10-20 minutes; then adjust the pH value to 6-8 with sodium hydroxide solution to obtain hyaluronic acid hydrogel; wash the hydrogel with deionized water in ultrasonic waves for 2-3 times, freeze Place in a freeze dryer for freeze-drying.
Embodiment 2
[0017] Example 2 Covalent crosslinking of hydrogel and laminin
[0018] (1) Take 3 to 4 g of the swollen hydrogel polymer, and wash them with 30 / 70, 60 / 40, and 80 / 20 acetone / DW solutions in turn, twice with pure acetone, and three times with dry acetone, gradually Dehydration of the hydrogel polymer and final storage in dry acetone;
[0019] (2) Add 5ml of 1,1'-carbonyldiimidazole (CDI) solution to the dry gel polymer prepared in step 1, and activate the reaction for 15 minutes under slight stirring. CDI is made into 150mg / mL with anhydrous acetone;
[0020] (3) Wash with dry acetone more than 5 times to remove unbound CDI, wash the CDI-activated gel 5 times with 100 mM sodium carbonate buffer solution (PH=8.5), and replace all the acetone solvent;
[0021] (4) Soak the CDI-activated hydrogel in 100 mM sodium bicarbonate buffer solution (0.6 mg / ml) at pH=8.5;
[0022] (5) mixing and stirring the laminin solution with the activated hydrogel at room temperature for 24 to 48 h...
Embodiment 3
[0026] Example 3 Characterization analysis of hydrogel
[0027] Observing the freeze-dried gel under a scanning electron microscope, it can be seen that it is a loose porous framework structure. The pores cover the whole gel, the walls of the pores are very thin, and there are direct or indirect connections between the pores. The diameter of each hole is about 100-200 μm. The porosity and pore size distribution of the gel in the dry state were detected by a mercury porosimeter, and the volume and pressure of liquid mercury intruding into the pores in the dry state were measured. The results showed that the pore size of the biological material was unevenly distributed between 0 and 1000 μm , most of the pores are concentrated between 20 and 200 μm, and the pores with a diameter of about 80 μm are the most, and the average porosity is 90%.
[0028] The above test results show that the hydrogel prepared according to the method of the present invention can accommodate a large nu...
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