1 results about "Cns injury" patented technology

This invention discloses a material-empowered stem cell-derived exosome, its preparation method, and its application, belonging to the field of biomedical technology. The method involves the following steps: mesenchymal stem cells are seeded onto a substrate fused with N / VE-cadherin-Fc fusion protein and cultured to assemble into aggregates; the aggregates are dynamically cultured under staged shear stress; the culture medium is collected, and the exosomes are separated and purified. The preparation method of this invention overcomes the shortcomings of existing mesenchymal stem cell exosome preparation technologies, such as low yield and insufficient neurovascular repair function. It significantly activates exosome biogenesis-related molecular pathways, increases exosome yield by approximately four times, and is rich in miRNAs related to neurovascular repair. It can significantly improve neurological deficits in stroke mice and hindlimb motor function in rats with spinal cord injury, providing a highly efficient and stable cell-free therapeutic strategy for the treatment of central nervous system injuries.