Method for fixing laminar adhesive protein on Ti-O film surface

A laminin and surface immobilization technology, applied in coating, medical science, prosthesis, etc., can solve the problems of laminin immobilization, and achieve poor blood compatibility, good endothelial cell compatibility, and wide application range. Effect

Inactive Publication Date: 2009-02-04
SOUTHWEST JIAOTONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the long-term stable immobilization of laminin usually requires active groups on the surface of the material that can react with it, such as: hydroxyl (-OH), amino (-NH2), carboxyl (-COOH); and Ti-O film is an inorganic material , cannot directly interact with biomolecules to immobilize them
There is no literature report on the immobilization of laminin to Ti-O thin film

Method used

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  • Method for fixing laminar adhesive protein on Ti-O film surface
  • Method for fixing laminar adhesive protein on Ti-O film surface
  • Method for fixing laminar adhesive protein on Ti-O film surface

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] see figure 1 , the first embodiment of the present invention is a method for immobilizing laminin on the surface of a Ti-O film, the steps of which are:

[0024] A. Cleaning of Ti-O film The anatase crystal Ti-O film was ultrasonically cleaned with acetone, ethanol, and distilled water in sequence for 10 minutes respectively to obtain a clean film surface, and put it into a clean room drying oven for drying;

[0025] B. Activation by Strong Alkali Under oscillating conditions at 50°C, put the clean Ti-O film into NaOH solution with a concentration of 10% WT and soak for 3 hours, then wash it with double distilled water, and then dry it in vacuum;

[0026] C, 98% (WT) H is activated by strong acid 2 SO 4 with 30% (WT) H 2 o 2 Prepare a mixed solution at a volume ratio of 1:1, soak the Ti-O film obtained in step B with the mixed solution for 2 hours at 90° C., then rinse with double-distilled water, and then vacuum-dry;

[0027] D. Coupling Prepare an ethanol solutio...

Embodiment 2

[0030] A method for immobilizing laminin on the surface of a Ti-O film, the steps of which are:

[0031] A. Cleaning of Ti-O film The Ti-O film of anatase and rutile mixed crystal form mainly composed of anatase is ultrasonically cleaned with acetone, ethanol, and distilled water for 8 minutes respectively to obtain a clean film surface. Dry in a clean room drying oven;

[0032] B. Activation by Strong Alkali Under the shaking condition of 80°C, put the clean Ti-O thin film into the NaOH solution with a concentration of 8% WT and soak for 1 hour, then wash it with double distilled water, and then dry it in vacuum;

[0033] C, 98% (WT) H is activated by strong acid 2 SO 4 with 30% (WT) H 2 o 2 Prepare a mixed solution at a volume ratio of 1:3, soak the Ti-O film obtained in step B with the mixed solution for 4 hours at 60° C., then rinse with double distilled water, and then vacuum-dry;

[0034] D. Coupling Prepare an ethanol solution of 3% wt r-aminopropyltriethoxysilane ...

Embodiment 3

[0037] A method for immobilizing laminin on the surface of a Ti-O film, the steps of which are:

[0038] A. Cleaning of Ti-O film The anatase crystal Ti-O film was ultrasonically cleaned with acetone, ethanol, and distilled water for 5 minutes respectively to obtain a clean film surface and dry;

[0039] B. Activation with strong alkali Under the condition of oscillating at 60°C, put the clean Ti-O film into NaOH solution with a concentration of 12% WT and soak it for 5 hours, then rinse it with double distilled water, and then dry it;

[0040] C, 98% (WT) H is activated by strong acid 2 SO 4 with 30% (WT) H2 o 2 Prepare a mixed solution at a volume ratio of 1:0.5, soak the Ti-O thin film obtained in step B with the mixed solution for 1 hour at 95° C., then rinse with double distilled water, and dry;

[0041] D. Coupling Prepare an ethanol solution of 1.5%wt r-aminopropyltriethoxysilane (APTE) as a coupling agent solution, and soak the Ti-O film obtained in step C into the ...

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Abstract

The invention discloses a method which fixes laminin on a Ti-O film surface. On the Ti-O film surface, the activation of alkali and strong acid can form reactive functional group hydroxyl -OH on the Ti-O film surface, and then silane coupling agent aminopropyl triethoxysilane (APTE) is used for forming a layer of coupling agent arranged between the Ti-O film surface and a bio-molecular layer which respectively have chemical bonding capacity, so as to improve the stability of the follow-up fixed biological molecules. Finally, water-soluble carbodiimide (EDC) is taken as the catalyst for fixing the protein and then fixing the laminin molecules, thereby firmly forming laminin biological gasification layer on the Ti-O film surface. As the laminin biological gasification layer is firmly combined and fixed on the Ti-O film surface, the blood compatibility and endothelial cell compatibility of the Ti-O film surface are improved obviously.

Description

Technical field [0001] The invention relates to an inorganic material surface modification technology, in particular to a biochemical modification method on the surface of an artificial organ material Ti-O thin film. Background technique [0002] Titanium-based metals have been widely used in the field of biomedicine, and the naturally formed Ti-O thin layer on the surface is considered to be the main reason for the good biocompatibility of titanium. However, in terms of clinical application, the biocompatibility of titanium-based metals is far from meeting clinical needs. [0003] Immobilization of biomacromolecules on the surface of biomaterials has become one of the best methods to improve their surface biocompatibility. In addition, the introduction of biospecific proteins that can be recognized by cell membrane receptors on the surface of materials will promote the integration of receptor-ligand effect and improve biocompatibility. Laminin (Ln) is the earliest extrace...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/06A61L27/34A61L31/12A61L31/02A61L31/10
Inventor 陈俊英黄楠葛胜男冷永祥杨苹王进孙鸿万国江赵安莎游天雪吴熹
Owner SOUTHWEST JIAOTONG UNIV
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