37 results about "Water soluble carbodiimide" patented technology

The invention relates to a double-component medical adhesive based on glucan and chitosan and a preparation method of the double-component medical adhesive. The double-component medical adhesive comprises formylated glucan and aminated carboxymethyl chitosan, wherein the mass ratio of the formylated glucan to the aminated carboxymethyl chitosan is (1.67-3.33):1. The preparation method includes: adding a sodium periodate solution into a glucan solution under a dark condition to perform dialysis, and performing freeze drying to obtain the formylated glucan; mixing carboxymethyl chitosan, ethylenediamine and water-soluble carbodiimide, and performing dialysis and freeze drying to obtain the aminated carboxymethyl chitosan; mixing the formylated glucan and aminated carboxymethyl chitosan which are identical in volume, and performing Schiff base reaction to form hydrogel to obtain the medical adhesive. The double-component medical adhesive and the preparation method thereof have the advantages that the raw materials of the double-component medical adhesive are nontoxic and odorless, cheap and good in biocompatibility; the preparation method is simple; the prepared medical adhesive is good in adhesive strength and capable of shortening gelation time, the medical adhesive can act on any body parts through simple local spraying or injection, and the medical adhesive is an ideal replacement product of commercially available fibrin glue.

A molecule possessing a primary or secondary amino group and an additional functionality capable of providing a novel or improved property to a cellulose material has been permanently attached to the cellulose material in aqueous media using a water-soluble carbodiimide as the coupling agent / activator. One such molecule is 5-aminofluorescein (abbreviated as “A-fluo”) and one such cellulose material is a papermaking pulp. Papers made from a pulp furnish containing, for example, 0.01 wt. % of the “A-fluo”-attached pulp show an embedded marker feature authenticable upon UV or visible light excitation. The “A-fluo”-attached pulp can also be used for the marking and identification of a pulp furnish.

A method for preparation of hyaluronic acid -chitose cross compatible material belongs to the biochemical technical sphere. The invention selects water-soluble carbodiimide as cross linker and N-hydroxy succinimide as active agent, adjusts PH value with N,N,N',N'-tetramethylethylenediamine, then makes the hyaluronic acid and the chitose perform cross-linking reaction, finally prepares the hyaluronic acid -chitose cross compatible material by dialysis and cryodesiccation. The material has a good application prospect in domains of drug embedment, tissue pack, film material and so on.

The invention discloses a method which fixes laminin on a Ti-O film surface. On the Ti-O film surface, the activation of alkali and strong acid can form reactive functional group hydroxyl -OH on the Ti-O film surface, and then silane coupling agent aminopropyl triethoxysilane (APTE) is used for forming a layer of coupling agent arranged between the Ti-O film surface and a bio-molecular layer which respectively have chemical bonding capacity, so as to improve the stability of the follow-up fixed biological molecules. Finally, water-soluble carbodiimide (EDC) is taken as the catalyst for fixing the protein and then fixing the laminin molecules, thereby firmly forming laminin biological gasification layer on the Ti-O film surface. As the laminin biological gasification layer is firmly combined and fixed on the Ti-O film surface, the blood compatibility and endothelial cell compatibility of the Ti-O film surface are improved obviously.

The invention provides a method for fixing a protein on the surface of polydimethylsiloxane. The method is characterized in that a biotinylated polydimethylsiloxane film is prepared on the solidified surface of polydimethylsiloxane, a protein is fixed on the surface of polydimethylsiloxane by chemical technologies of biotin-avidin and water-soluble carbodiimide and a technology of 3-mercaptopropionic acid stabilized gold nano-particles, and beta-D-dodecyl-N-maltose and bovine serum albumin are utilized respectively for blockading the non-specific adsorption of a protein on the surface of polydimethylsiloxane and activated residual carboxylic groups on the surface of a 3-mercaptopropionic acid stabilized gold nano-particle. In the invention, the surface of a 3-mercaptopropionic acid stabilized gold nano-particle contains multiple carboxylic groups which can combine molecules of multiple proteins needing to be fixed thus multiple proteins needing to be fixed can be fixed on the surface of polydimethylsiloxane and a sensitivity of a subsequent enzyme-linked immuno sorbent assay (ELISA) is improved effectively.

A method for preparing 3 ¿C chlorine ¿C 1, 2 ¿C propanediol artificial antigen includes using 3 ¿C chloro ¿C lactic acid as hapten, using water soluble carbon diimine hydrochloride as coupling agent and coupling hapten of 3 ¿C chloro ¿C lactic acid with cationization carrier to prepare out 3 ¿C chlorine , 2 ¿C propanediol artificial antigen.

The present invention relates to polymeric antitumor agent which is formed in polymeric micelle complex by intermolecular bonding or mutual interaction between styrene maleic acid copolymer (SMA) and low molecule antitumor agent which is anthracyclins drug such as pirarubicin, doxorubicm, epirbicin, daunorbicin, acralbicin, or alkaloid antitumor agent such as cis-platinum, and taxol These polymeric antitumor agents may improve specificity to cancer cells so that it improves antitumor effect, while it may not be concentrated at normal organ or tissue, so that adverse effect may be diminished. These polymeric antitumor agents may be prepared by dissolving SMA and low molecule antitumor agent in aqueous solution, then in the presence of aqueous soluble carbodiimide, amino acids, or polyamine, adjusting pH to form micelle complex and recovering polymer fraction.

The invention discloses an organophosphorus detection method based on interference spectrum analysis, belongs to the technical field of analytical chemistry detection and optical fiber biosensors, andrelates to a method for detecting organophosphorus in a sample solution by modifying the optical fiber biosensors. The method is characterized in that a water-soluble carbodiimide method is used forpreparing an organophosphorus universal hapten, and then the organophosphorus universal hapten is coupled with bovine serum albumin and chicken egg white albumin respectively; and the obtained conjugate is used for modifying an optical fiber biosensor, and then the optical fiber biosensor is used for determining the organophosphorus content in the sample solution. The detection method has the advantages of simplicity and convenience, short detection period, fast response time, good stability, high sensitivity and the like, and a modified probe is reproducible for reuse, so that the detectioncost is low, and large-scale popularization and application are expected to be carried out.

Provided is an aluminum fin material in which the hydrophilicity of a fin material surface is sustained for a long period time. A fin material (10A) having a hydrophilic film layer (2) and a lubricant film layer (3) provided to the surface of an aluminum plate (1) in the stated order, the fin material being characterized in that: the hydrophilic film layer (2) is composed of a polymer configured solely from a monomer having a carboxyl group, a copolymer containing a monomer having a carboxyl group, or a resin substance containing a hydrophilic resin as a mixture thereof; and the lubricant film layer (3) is composed of a resin composition in which a crosslinking agent (6) has a solids ratio of 0.2 to 70 mass%, the lubricant film layer (3) containing one or more lubricant resins selected from the group consisting of polyethylene glycol, carboxymethyl cellulose, and an alkali metal salt of carboxymethyl cellulose, and one or more crosslinking agents (6) selected from the group consisting of a water-soluble epoxy resin, a water-soluble carbodiimide compound, a water-dispersible carbodiimide compound, and a water-soluble oxazoline group-containing resin.

The invention relates to an alginate hydrogel microcarrier and a preparation method thereof. With a molecular structure represented by the formula 1, the alginate hydrogel microcarrier is prepared by the steps of: under the activation of water-soluble carbodiimide, carboxyl in sodium alginate and amino in cystamine undergo an amidation reaction by a suspension crosslinking method so as to generate a chemically crosslinked alginate hydrogel microcarrier, freeze drying the chemically crosslinked alginate hydrogel microcarrier so as to obtain a porous structured microcarrier which is adhered with chitosan, heparin, basic fibroblast growth factors, fibronectins and other components. The alginate hydrogel microcarrier provided in the invention has simple preparation method, abundant raw material source, good degradability in vitro and cellular compatibility. Application of the alginate hydrogel microcarrier of the invention to mesenchymal stem cell amplification in vitro can avoid the damage of a pancreatin digestion process to cells, and can also increase the cell output.

Bioimplants and methods of making the bioimplants are provided. The bioimplants comprise biological tissues having conjugated thereto adjunct molecules. The biological tissues are sterilized with a chemical sterilizing agent, such as a water soluble carbodiimide. The processes of making the bioimplants include a process in which an adjunct molecule is conjugated to a biological tissue during the sterilization process.

A method for preparation of hyaluronic acid -chitose cross compatible material belongs to the biochemical technical sphere. The invention selects water-soluble carbodiimide as cross linker and N-hydroxy succinimide as active agent, adjusts PH value with N,N,N',N'-tetramethylethylenediamine, then makes the hyaluronic acid and the chitose perform cross-linking reaction, finally prepares the hyaluronic acid -chitose cross compatible material by dialysis and cryodesiccation. The material has a good application prospect in domains of drug embedment, tissue pack, film material and so on.