37 results about "Water soluble carbodiimide" patented technology

The invention relates to a chitosan modified alginate hydrogel three-dimensional porous bracket with specific in-vitro degradability and a preparation method thereof. The method comprises the following steps: dissolving sodium alga acid serving as a raw material in phosphate buffer solution; performing amidation reaction of a carboxyl group in the sodium alga acid and an amino group in a cross-linking agent cystamine or dimethyl cystinate under the activation of water-soluble carbodiimide to form a chemically crosslinked hydrogel; performing freeze drying on the hydrogel to obtain a porous bracket material of the hydrogel; and performing surface modification on the porous bracket by using chitosan. In solution of a reducing agent such as cysteine with an appropriate concentration, a disulfide bond in a hydrogel cross-bridge is degraded through a disulfide bond-sulfydryl conversion reaction, so the porous bracket is decomposed and dissolved and disappears. Therefore, the porous bracket can be used as an in-vitro cell culture template material. The hydrogel porous bracket researched by the invention has the characteristics of simple preparation, rich raw material source, low cost and availability. Various physiochemical performances, mechanical strength, degradation rate and surface properties of the bracket material are controllable within a large range.

The invention relates to a preparation method of an aceptic packaging material with a release-controlling function, particularly comprising three steps: firstly, a chemical surface modifying method is adopted to cause the surface of polymer packaging materials to form certain amount of dissociable functional groups such as carboxyl, sulfonic group, amidine, etc; meanwhile, antibacterial agent nisin is coated inside chitosan hydrogel microspheres which employ carboxyl polyethylene glycol as crosslinking agent, employ water-soluble carbodiimide hydrochloride as coupling agent and have particle size smaller than 100nm; in mixed solvent with 100 / 0-50 / 50 of water / ethanol, the drug-coating chitosan microspheres through ultrasonic stirring processing is 'fixed' on the surface of modified polymer thin films through electrostatic absorption between cathodic anodic ion. Since the surface modifying methods and chitosan hydrogel formulas are different, the releasable nisin amount can reach 0.5-40 Mu g / cm<2>.

The invention relates to a double-component medical adhesive based on glucan and chitosan and a preparation method of the double-component medical adhesive. The double-component medical adhesive comprises formylated glucan and aminated carboxymethyl chitosan, wherein the mass ratio of the formylated glucan to the aminated carboxymethyl chitosan is (1.67-3.33):1. The preparation method includes: adding a sodium periodate solution into a glucan solution under a dark condition to perform dialysis, and performing freeze drying to obtain the formylated glucan; mixing carboxymethyl chitosan, ethylenediamine and water-soluble carbodiimide, and performing dialysis and freeze drying to obtain the aminated carboxymethyl chitosan; mixing the formylated glucan and aminated carboxymethyl chitosan which are identical in volume, and performing Schiff base reaction to form hydrogel to obtain the medical adhesive. The double-component medical adhesive and the preparation method thereof have the advantages that the raw materials of the double-component medical adhesive are nontoxic and odorless, cheap and good in biocompatibility; the preparation method is simple; the prepared medical adhesive is good in adhesive strength and capable of shortening gelation time, the medical adhesive can act on any body parts through simple local spraying or injection, and the medical adhesive is an ideal replacement product of commercially available fibrin glue.

Tissue adhesives formed by crosslinking albumin and / or gelatin with certain polyamines and / or polycarboxylates using a water-soluble carbodiimide are disclosed. The use of the tissue adhesives for medical and veterinary applications such as topical wound closure; and surgical procedures, such as intestinal anastomosis, vascular anastomosis, tissue repair, and ophthalmic procedures; drug delivery; anti-adhesive applications; and as a bulking agent to treat urinary incontinence are described.

The invention relates to polyethylene glycol monomethyl ether-poly 2-methyl-carboxyl propylene carbonate graft polyethyleneimine copolymer, a preparation method thereof and application thereof. The preparation method comprises the step of directly condensing carboxyl in the polyethylene glycol monomethyl ether-poly 2-methyl-carboxyl propylene carbonate graft polyethyleneimine segmented copolymer with amino in polyethyleneimine to form the graft copolymer. The copolymer is a polycation gene carrier, integrates the advantages of polyethylene glycol, Makrolan and polyethyleneimine, and has high transfection efficiency, wherein the highest transfection efficiency to the medium luciferase plasmid of african green monkey kidney cell is 14 times of that of American Invitrogen biological company transfection reagent Lipofetamine<TM>2000, can effectively antagonize the inhibiting effect of blood serum to the transfection, and has less cell toxicity, wherein consistency thereof is not higher than 200 microgramme / ml and cell survival rate is more than 80%.

Bioimplants and methods of making the bioimplants are provided. The bioimplants comprise biological tissues having conjugated thereto adjunct molecules. The biological tissues are sterilized with a chemical sterilizing agent, such as a water soluble carbodiimide. The processes of making the bioimplants include a process in which an adjunct molecule is conjugated to a biological tissue during the sterilization process.

A molecule possessing a primary or secondary amino group and an additional functionality capable of providing a novel or improved property to a cellulose material has been permanently attached to the cellulose material in aqueous media using a water-soluble carbodiimide as the coupling agent / activator. One such molecule is 5-aminofluorescein (abbreviated as “A-fluo”) and one such cellulose material is a papermaking pulp. Papers made from a pulp furnish containing, for example, 0.01 wt. % of the “A-fluo”-attached pulp show an embedded marker feature authenticable upon UV or visible light excitation. The “A-fluo”-attached pulp can also be used for the marking and identification of a pulp furnish.

A method for preparation of hyaluronic acid -chitose cross compatible material belongs to the biochemical technical sphere. The invention selects water-soluble carbodiimide as cross linker and N-hydroxy succinimide as active agent, adjusts PH value with N,N,N',N'-tetramethylethylenediamine, then makes the hyaluronic acid and the chitose perform cross-linking reaction, finally prepares the hyaluronic acid -chitose cross compatible material by dialysis and cryodesiccation. The material has a good application prospect in domains of drug embedment, tissue pack, film material and so on.

The invention provides a microalbuminuria (mAlb) detection kit, which is based on a latex enhanced turbidimetric immunoassay method, is a liquid double reagent, namely reagent R1 and reagent R2, wherein the reagent R1 is a reactant and the reagent R2 is a solution containing albumin immuno latex particles, and the detection kit is characterized by crosslinking by applying a chemical process, and covalently crosslinking a goat anti-human albumin antibody (also called goat anti-human mAlb antibody) with carboxylated polystyrene latex through water-soluble carbodiimide (EDC) and H-hydroxy succinimide (NHS), so as to form an mAlb antibody latex reagent. The reagent has the advantages of high sensitivity, strong specificity and simple preparation, and is worthy of further promotion.

This invention discloses a latex agglutination kit to quickly detect H5 subtype of avian influenza virus. The anti monoclonal antibodies of H5 subtype of avian influenza virus hemagglutinin protein were coupled to the surface of carboxyl polystyrene latex particles using water-soluble carbodiimide, the methods about detection of H5 subtype of avian influenza virus were established, the latex agglutination detection kits of H5 subtype of avian influenza virus were prepared supplemented by other matching reagents. This kit was made up of box body and the body latex diagnostic reagents in the box, sample handling liquid A, sample handling liquid B, the positive control samples and negative control samples. This invention kit can directly detect the H5 subtype avian flu virus, with high specificity, high sensitivity, simple and rapid diagnosis, and other significant advantages.

The invention discloses a method which fixes laminin on a Ti-O film surface. On the Ti-O film surface, the activation of alkali and strong acid can form reactive functional group hydroxyl -OH on the Ti-O film surface, and then silane coupling agent aminopropyl triethoxysilane (APTE) is used for forming a layer of coupling agent arranged between the Ti-O film surface and a bio-molecular layer which respectively have chemical bonding capacity, so as to improve the stability of the follow-up fixed biological molecules. Finally, water-soluble carbodiimide (EDC) is taken as the catalyst for fixing the protein and then fixing the laminin molecules, thereby firmly forming laminin biological gasification layer on the Ti-O film surface. As the laminin biological gasification layer is firmly combined and fixed on the Ti-O film surface, the blood compatibility and endothelial cell compatibility of the Ti-O film surface are improved obviously.

The invention provides a method for fixing a protein on the surface of polydimethylsiloxane. The method is characterized in that a biotinylated polydimethylsiloxane film is prepared on the solidified surface of polydimethylsiloxane, a protein is fixed on the surface of polydimethylsiloxane by chemical technologies of biotin-avidin and water-soluble carbodiimide and a technology of 3-mercaptopropionic acid stabilized gold nano-particles, and beta-D-dodecyl-N-maltose and bovine serum albumin are utilized respectively for blockading the non-specific adsorption of a protein on the surface of polydimethylsiloxane and activated residual carboxylic groups on the surface of a 3-mercaptopropionic acid stabilized gold nano-particle. In the invention, the surface of a 3-mercaptopropionic acid stabilized gold nano-particle contains multiple carboxylic groups which can combine molecules of multiple proteins needing to be fixed thus multiple proteins needing to be fixed can be fixed on the surface of polydimethylsiloxane and a sensitivity of a subsequent enzyme-linked immuno sorbent assay (ELISA) is improved effectively.

The invention provides a complement C3 detection method, which is based on the latex-enhanced immune turbidimetry. In the detection method, two liquid reagents (reagent R1 and reagent R2) are used, the reagent R1 comprises a phosphate buffer solution (pH=6), tween 20, disodium ethylene diamine tetraacetic acid (EDTA-2Na), sodium chloride, polyethylene glycol (PEG6000) and sodium azide; and the reagent R2 is a solution containing complement C3 latex particles. The detection method is characterized in that chemical crosslinking is used, and through the water-soluble carbodiimide (EDC) and N-hydroxy succinimide (NHS), the goat anti-human complement C3 antiserum can carry out covalent crosslinking with carboxylated polystyrene latex to form a complement C3 latex reagent. The complement C3 latex reagent has the advantages of high sensitivity, strong specificity, and simple preparation, and thus is worthy to promote and use.

A molecule possessing a primary or secondary amino group and an additional functionality capable of providing a novel or improved property to a cellulose material has been permanently attached to the cellulose material in aqueous media using a water-soluble carbodiimide as the coupling agent / activator. One such molecule is 5-aminofluorescein (abbreviated as “A-fluo”) and one such cellulose material is a papermaking pulp. Papers made from a pulp furnish containing, for example, 0.01 wt. % of the “A-fluo”-attached pulp show an embedded marker feature authenticable upon UV or visible light excitation. The “A-fluo”-attached pulp can also be used for the marking and identification of a pulp furnish.

The invention provides a complement C3 detection kit, which is a liquid double reagent, including a reagent R1 and a reagent R2; the reagent R1 contains pH 6.0 phosphate buffered saline, Tween 20, Ethylene Diamine Tetraacetic Acid (EDTA-2Na), sodium chloride, polyethylene glycol (PEG 6000) and sodium azide; the reagent R2 is a solution containing complement C3 latex particles. The complement C3 detection kit is characterized in that by crosslinking by using a chemical process, water-soluble carbodiimide (EDC) and N-hydroxysuccinimide (NHS) are utilized to enable goat anti-human complement C3 antiserum and carboxylated polystyrene latex to be subjected to covalent cross linking, thus forming the complement C3 latex reagent. The reagent is high in sensitivity, high in specificity, simple in reagent preparation, and worth of being popularized and used further.

The invention relates to a synthesis method of a highly specific folic acid complete antigen and an application of the folic acid complete antigen and belongs to the technical field of biochemical industry. The synthesis method disclosed by the invention comprises the steps of adding 6-aminocaproic acid and water-soluble carbodiimide into a vector protein solution and then reacting at a room temperature and carrying out dialysis to obtain a vector protein B modified by a linker arm, dissolving folic acid(N-{4-[(2-amino-1,4-dihydro-4-oxo-6-pteridinyl)methylamino]benzoyl}-L-glutamic acid) in dimethyl sulfoxide, adding dicyclohexyl carbodiimide and hydroxysuccinimide, reacting to obtain a folic acid derivative and then coupling the folic acid derivative with an amino group on the linker arm of the vector protein B to obtain the complete antigen. The results show that the titer of the antiserum obtained by immunizing animals with the antigen disclosed by the invention reaches up to 81000, the detection limit is 0.042ng / mL, and the half-inhibitory concentration is 0.3ng / mL. The produced antibody has high specificity and high sensitivity. The antigen or antibody disclosed by the invention is used for preparing an immunoaffinity column, establishing an enzyme linked immunosorbent assay method and colloidal gold strip rapid detection method and detecting the residue of folic acid.

A method for preparing 3 ¿C chlorine ¿C 1, 2 ¿C propanediol artificial antigen includes using 3 ¿C chloro ¿C lactic acid as hapten, using water soluble carbon diimine hydrochloride as coupling agent and coupling hapten of 3 ¿C chloro ¿C lactic acid with cationization carrier to prepare out 3 ¿C chlorine , 2 ¿C propanediol artificial antigen.

The present invention relates to polymeric antitumor agent which is formed in polymeric micelle complex by intermolecular bonding or mutual interaction between styrene maleic acid copolymer (SMA) and low molecule antitumor agent which is anthracyclins drug such as pirarubicin, doxorubicm, epirbicin, daunorbicin, acralbicin, or alkaloid antitumor agent such as cis-platinum, and taxol These polymeric antitumor agents may improve specificity to cancer cells so that it improves antitumor effect, while it may not be concentrated at normal organ or tissue, so that adverse effect may be diminished. These polymeric antitumor agents may be prepared by dissolving SMA and low molecule antitumor agent in aqueous solution, then in the presence of aqueous soluble carbodiimide, amino acids, or polyamine, adjusting pH to form micelle complex and recovering polymer fraction.

The invention discloses an anti-GOLPH2-lipoplexes liposome carrier, which is prepared from activated cationic liposome lipoplexes and activated anti-GOLPH2 antibodies through crosslinking by water-soluble carbodiimide. The anti-GOLPH2-lipoplexes liposome carrier belongs to a targeted non-virus liposome carrier. The liposome carrier is used for covering recombinant plasmids pBI-Sur-TK to form a compound; the dual targeted and control treatment on the liver cancer is performed; the targeted enrichment and expression of the treatment gene in liver cancer cells are realized; the killing effect onthe liver cancer cells is enhanced; the injury to the normal liver cells is reduced.

The invention provides a detection method for microalbuminuria (mAlb). The detection method for microalbuminuria is based on the latex-enhanced immunoturbidimetric assay, and adopts a liquid double reagent comprising a reagent R1 and a reagent R2, wherein the reagent R1 serves as a reactant; the reagent R2 is a solution containing albumin immuno-latex particles. The detection method is characterized by applying a chemical method for cross-linking and preparing an mAlb antibody latex reagent via covalent cross-linking between goat anti-human albumin antibody (also called as goat anti-human mAlb antibody) and carboxylated polystyrene by using water-soluble carbodiimide (EDC) and N-hydroxy succinimide (NHS). The mAlb antibody latex reagent is high in sensitivity and specificity, simple to prepare, and worth of further popularization and use.

The invention discloses an organophosphorus detection method based on interference spectrum analysis, belongs to the technical field of analytical chemistry detection and optical fiber biosensors, andrelates to a method for detecting organophosphorus in a sample solution by modifying the optical fiber biosensors. The method is characterized in that a water-soluble carbodiimide method is used forpreparing an organophosphorus universal hapten, and then the organophosphorus universal hapten is coupled with bovine serum albumin and chicken egg white albumin respectively; and the obtained conjugate is used for modifying an optical fiber biosensor, and then the optical fiber biosensor is used for determining the organophosphorus content in the sample solution. The detection method has the advantages of simplicity and convenience, short detection period, fast response time, good stability, high sensitivity and the like, and a modified probe is reproducible for reuse, so that the detectioncost is low, and large-scale popularization and application are expected to be carried out.

An object of the present invention is to provide an easy-adhesive composition that not only suppresses deterioration in initial performance when used for a long time under high temperature and high humidity, but also satisfies moisture and heat resistance in more severe environments, and a thermoplastic resin film in which An optical film in which an easy-adhesion layer formed of the easy-adhesion composition is provided on at least one surface. The invention provides an easy-adhesive composition, which contains: 100 parts by weight of water-dispersible polyurethane resin with carboxyl groups, 1-25 parts by weight of water-soluble carbodiimide compounds, and 1-25 parts by weight of water-dispersible carbon dioxide. An imine compound; an optical film in which an easy-adhesion layer formed of the easy-adhesion composition is provided on at least one surface of a thermoplastic resin film.

The invention discloses a preparation method of long-acting antifouling emulsion paint for exterior walls and belongs to the technical field of building materials. The preparation method comprises thefollowing steps: modifying polystyrene microspheres with concentrated sulfuric acid to prepare sulfonated polystyrene microspheres, making the sulfonated polystyrene microspheres into modified polystyrene microspheres with hydrophilic surface and hydrophobic inside by using a pore-foaming agent and modified sodium lignosulphonate, grafting a flexible segment containing a hydroxyl group onto the surface of nano-silica by using isocyanate, epoxy resin and organic amine, adding the modified polystyrene microspheres and modified silica to the product, simultaneously adding modified peanut shell fiber, a treating agent and water-soluble carbodiimide so as to prepare the long-acting antifouling emulsion paint for exterior walls. The prepared emulsion paint has excellent hydrophilicity on the surface, and the hydrophilic substance is not easy to loss. Thereby, the product has long-acting fouling resistance.

Provided is an aluminum fin material in which the hydrophilicity of a fin material surface is sustained for a long period time. A fin material (10A) having a hydrophilic film layer (2) and a lubricant film layer (3) provided to the surface of an aluminum plate (1) in the stated order, the fin material being characterized in that: the hydrophilic film layer (2) is composed of a polymer configured solely from a monomer having a carboxyl group, a copolymer containing a monomer having a carboxyl group, or a resin substance containing a hydrophilic resin as a mixture thereof; and the lubricant film layer (3) is composed of a resin composition in which a crosslinking agent (6) has a solids ratio of 0.2 to 70 mass%, the lubricant film layer (3) containing one or more lubricant resins selected from the group consisting of polyethylene glycol, carboxymethyl cellulose, and an alkali metal salt of carboxymethyl cellulose, and one or more crosslinking agents (6) selected from the group consisting of a water-soluble epoxy resin, a water-soluble carbodiimide compound, a water-dispersible carbodiimide compound, and a water-soluble oxazoline group-containing resin.

Provided is an aluminum fin material in which the hydrophilicity of a fin material surface is sustained for a long period time. A fin material (10A) having a hydrophilic film layer (2) and a lubricant film layer (3) provided to the surface of an aluminum plate (1) in the stated order, the fin material being characterized in that: the hydrophilic film layer (2) is composed of a polymer configured solely from a monomer having a carboxyl group, a copolymer containing a monomer having a carboxyl group, or a resin substance containing a hydrophilic resin as a mixture thereof; and the lubricant film layer (3) is composed of a resin composition in which a crosslinking agent (6) has a solids ratio of 0.2 to 70 mass%, the lubricant film layer (3) containing one or more lubricant resins selected from the group consisting of polyethylene glycol, carboxymethyl cellulose, and an alkali metal salt of carboxymethyl cellulose, and one or more crosslinking agents (6) selected from the group consisting of a water-soluble epoxy resin, a water-soluble carbodiimide compound, a water-dispersible carbodiimide compound, and a water-soluble oxazoline group-containing resin.

The invention relates to an alginate hydrogel microcarrier and a preparation method thereof. With a molecular structure represented by the formula 1, the alginate hydrogel microcarrier is prepared by the steps of: under the activation of water-soluble carbodiimide, carboxyl in sodium alginate and amino in cystamine undergo an amidation reaction by a suspension crosslinking method so as to generate a chemically crosslinked alginate hydrogel microcarrier, freeze drying the chemically crosslinked alginate hydrogel microcarrier so as to obtain a porous structured microcarrier which is adhered with chitosan, heparin, basic fibroblast growth factors, fibronectins and other components. The alginate hydrogel microcarrier provided in the invention has simple preparation method, abundant raw material source, good degradability in vitro and cellular compatibility. Application of the alginate hydrogel microcarrier of the invention to mesenchymal stem cell amplification in vitro can avoid the damage of a pancreatin digestion process to cells, and can also increase the cell output.

Bioimplants and methods of making the bioimplants are provided. The bioimplants comprise biological tissues having conjugated thereto adjunct molecules. The biological tissues are sterilized with a chemical sterilizing agent, such as a water soluble carbodiimide. The processes of making the bioimplants include a process in which an adjunct molecule is conjugated to a biological tissue during the sterilization process.

A method for preparation of hyaluronic acid -chitose cross compatible material belongs to the biochemical technical sphere. The invention selects water-soluble carbodiimide as cross linker and N-hydroxy succinimide as active agent, adjusts PH value with N,N,N',N'-tetramethylethylenediamine, then makes the hyaluronic acid and the chitose perform cross-linking reaction, finally prepares the hyaluronic acid -chitose cross compatible material by dialysis and cryodesiccation. The material has a good application prospect in domains of drug embedment, tissue pack, film material and so on.

The invention relates to a mask for slowing down respiratory tract infectious diseases and a using method thereof, which are characterized in that hydroxyl on cellulose in gauze is introduced into carboxyl under an alkaline condition through a sodium chloroacetate method, and the introduced carboxyl is covalently coupled with amino in trypsin under the action of a water-soluble carbodiimide cross-linking agent to obtain the mask for slowing down the respiratory tract infectious diseases; the trypsin is covalently bound to the gauze to prepare the immobilized trypsin. According to the immobilized enzyme technology, protease is immobilized on gauze to form immobilized enzyme. A solution with the pH of 7.2-8.0 is filled in a small plastic spray bottle to spray steam mist to gauze, so the pancreatin is in a moist environment, the solid-phase pancreatin is in the optimum working pH range, and then the pancreatin is kept at a proper temperature through human respiration. The pancreatin destroys spike protein of protein on a surface of a new crown under proper conditions, so the pancreatin cannot be combined with a virus receptor in a human body, and the prevention and control effect of blocking the new crown virus is improved.