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Organophosphorus detection method based on interference spectrum analysis

A detection method and interference spectroscopy technology, which are applied in the field of organophosphorus detection based on interference spectroscopy analysis, can solve the problems of false negative/positive by immune method, high cost of instruments and equipment, influence of enzyme inhibition method, etc.

Inactive Publication Date: 2018-03-30
陈军 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although various detection methods have their specific advantages, there are also disadvantages, such as: the traditional chromatographic detection method has high equipment costs, and the pretreatment of samples is more complicated; the immunological method may produce false negative / positive phenomena; the enzyme inhibition method is also can be affected by enzymatic activity

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  • Organophosphorus detection method based on interference spectrum analysis

Examples

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Embodiment 1

[0026] Example 1: A detection method for organophosphorus based on interference spectrum analysis

[0027] Synthesis of general immunogen and coating agent of organophosphorus pesticides:

[0028] Synthesis of coated original conjugates: Weigh 0.88g of 4-hydroxybenzoic acid and dissolve it in 50 mL of methanol containing 0.90 g of KOH, add dropwise (within 5min) 1.81g of diethoxyphosphorous thiol under stirring at 0°C acid chloride, heated to 65°C, and stirred for 18 h. After the reaction was completed, the reactant was concentrated by rotary evaporation, and the obtained crude product was dissolved in ethyl acetate (20-30mL), extracted three times with 50mL 2mol / L NaOH solution, and the aqueous phase was acidified with 6mol / L hydrochloric acid until precipitation occurred. Washing with water yielded 1.05 g of the hapten. Dissolve 0.12 mmol of hapten in 2 mL of DMF, stir and add 0.14 mmol each of N,N'-dicyclohexylcarbodiimide (DCC) and N-hydroxysuccinimide (NHS), and react w...

Embodiment 2

[0035] Example 2: A detection method for organophosphorus based on interference spectrum analysis

[0036] A rapid test method for residual organophosphorus pesticides, in which a detection device is used, the detection device includes a sample pool, an optical fiber biosensor modified by a substrate carrier, an optical fiber connector and a spectrum analyzer, and is characterized by The general organophosphorus hapten prepared by the water-soluble carbodiimide method is then coupled with bovine serum albumin and chicken ovalbumin respectively, and the obtained conjugates are modified on the optical fiber biosensor to measure the content of organophosphate in the sample solution . The optical fiber biosensor modified by the substrate carrier has a carrier conjugate immobilized on the surface of the bottom of the fiber optic probe, and the conjugate is prepared by coupling with bovine serum albumin with diethylphosphonic acid acetic acid as a hapten. Originally, the modified f...

Embodiment 3

[0051] Example 3: A detection method for organophosphorus based on interference spectrum analysis

[0052] An optical sensor detection method for rapid detection of residual organophosphorus pesticides, said method comprising the steps of:

[0053] Synthesis of immunogen conjugates: Weigh 0.88g of 4-hydroxybenzoic acid and dissolve it in 50 mL of methanol containing 0.90 g of KOH, add dropwise (within 5min) 1.81g of diethoxyphosphoryl thiochloride under stirring at 0°C , heated to 65°C, and stirred for 18 h. After the reaction was completed, the reactant was concentrated by rotary evaporation, and the obtained crude product was dissolved in ethyl acetate (20-30mL), extracted three times with 50mL 2mol / L NaOH solution, and the aqueous phase was acidified with 6mol / L hydrochloric acid until precipitation occurred. Washing with water yielded 1.05 g of the hapten. Dissolve 0.12 mmol of hapten in 2 mL of DMF, stir and add 0.14 mmol each of N,N'-dicyclohexylcarbodiimide (DCC) and ...

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Abstract

The invention discloses an organophosphorus detection method based on interference spectrum analysis, belongs to the technical field of analytical chemistry detection and optical fiber biosensors, andrelates to a method for detecting organophosphorus in a sample solution by modifying the optical fiber biosensors. The method is characterized in that a water-soluble carbodiimide method is used forpreparing an organophosphorus universal hapten, and then the organophosphorus universal hapten is coupled with bovine serum albumin and chicken egg white albumin respectively; and the obtained conjugate is used for modifying an optical fiber biosensor, and then the optical fiber biosensor is used for determining the organophosphorus content in the sample solution. The detection method has the advantages of simplicity and convenience, short detection period, fast response time, good stability, high sensitivity and the like, and a modified probe is reproducible for reuse, so that the detectioncost is low, and large-scale popularization and application are expected to be carried out.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry and optical biosensing, and in particular relates to an organic phosphorus detection method based on interference spectrum analysis, which can quickly and effectively realize the detection of organic phosphorus. Background technique [0002] Organophosphorus pesticides have outstanding characteristics such as high efficiency and broad spectrum, special mechanism of action, relatively small environmental residues, safety to crops, many varieties, slow resistance development, low production costs, and special antidotes, whether they are widely used In terms of the share of sales volume, it ranks first among all kinds of pesticides and occupies a pivotal position in the pesticide market. While organophosphorus pesticides play a huge role in the prevention and control of crop diseases and insect pests and the elimination of pests, due to excessive use, it will inevitably cause serious pol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25G01N1/28
Inventor 陈军王玥张嵘
Owner 陈军
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