20 results about "Fiber optic biosensor" patented technology

The invention belongs to the technical field of biological detection, and particularly relates to a multi-channel optical fiber biosensor based on fluorescence detection, optical fiber sensing units can be cascaded on the same optical fiber, and the sensing units are realized through a packaging structure adopting 3D printing resin. Quantum dot modification, functionalization processing and sensing detection on the surface of the sensing area of the optical fiber sensing unit can be completed in the packaging structure, and integration is achieved. Different antibody materials can be modified on quantum dot materials with different particle sizes so as to obtain fluorescence signals with different emission wavelengths, and qualitative and quantitative detection of various microorganisms and viruses is realized according to the variation of fluorescence intensity of a corresponding emission peak. The sensor is low in cost, short in preparation time and simple in signal acquisition and processing, the packaging structure can ensure the stability of the sensor, the sensor is convenient to carry, and the sensor can be applied to the environmental protection field such as outdoor water quality real-time monitoring.

The invention provides a detection method of an end face integrated reflection type SPR optical fiber biosensor manufactured based on a 2PP technology. An experiment system mainly comprises a single-mode optical fiber, an optical fiber coupler, an SPR probe, a tungsten lamp light source, a sample pool, a spectrum analyzer and a computer. The method is characterized in that a volcanic three-dimensional microstructure is directly constructed on the end face of a single-mode fiber through a 2PP technology, a metal film with the thickness of about 50nm is deposited on the inner wall of the volcanic three-dimensional microstructure, and a stable and efficient SPR excitation structure is formed; the structural parameters are subjected to FDTD simulation optimization design, and have the characteristics of high quality factor and high sensitivity. The sensor can be used for AFP detection, the sensitivity reaches 0.684 nm / (ng / mL) within the linear range of 0-10 ng / mL, the normal and risk early warning concentration interval of human serum AFP is covered, and the sensor has good biological suitability and early hepatocellular carcinoma screening capacity; in addition, the probe structure is small in size, detection can be completed only by a few microliters of liquid to be detected, and the probe is suitable for a microfluidic platform with an extremely low sample size or a clinical sampling scene. Compared with the prior art, the device has the advantages of high structural integration, high sensitivity, low sample consumption, convenience in manufacturing, stability, reliability and the like.

The invention provides an optical fiber biosensor, a preparation method thereof and an optical fiber sensing system.The optical fiber biosensor comprises an optical fiber microstructure with an optical fiber sensing part and a surface plasma resonance layer, and the outer surface of the optical fiber sensing part is coated with the surface plasma resonance layer; the surface plasma resonance layer is used for exciting the optical fiber sensing part to generate surface plasma waves and combining or adsorbing a sensing target, and the surface plasma resonance layer is an AuNPs (at) MOF composite film; the AuNPs (at) MOF composite film is combined on the outer surface of the optical fiber sensing part through a chemical modification method, the optical fiber sensing part can be excited to generate surface plasma waves, and a sensing target is combined or adsorbed, so that a sensing film corresponding to a target detection object does not need to be coated during follow-up biological sensing, and the cost is reduced. And the manufacturing cost of the optical fiber biosensor based on the LSPR effect is further reduced.

The invention belongs to the technical field of optical fiber biosensors, and discloses an optical fiber biosensor for multi-parameter measurement, which comprises a sensor main body, the sensor main body is a fiber core made of a photonic crystal material, and the periphery of the fiber core is wrapped with a cladding. A plurality of circular air holes of two different sizes are etched in the cladding, two pairs of elliptical holes perpendicular to each other are formed in the portion, close to the fiber core, of the cladding, a first sensing channel is formed by a pair of elliptical holes which are symmetrical left and right, and a second sensing channel is formed by a pair of elliptical holes which are symmetrical up and down. By generating surface plasma resonance (SPR) and loss mode resonance (LMR) effects and utilizing coupling of a waveguide mode and a specific loss mode, simultaneous measurement of multiple parameters such as temperature, solution refractive index, biological solution concentration and the like is realized.

The invention relates to the field of biochemical sensing, in particular to a nucleic acid aptamer biosensor, an optical fiber sensor and cell detection application. The nucleic acid aptamer biosensor comprises a matrix, a connecting structure and TDN-coated AuNP, the matrix and the TDN-coated AuNP are combined through the connecting structure, and the connecting structure is an amino group, PolyA or a functionalized complementary chain. Through a special tetrahedral nanogold structure, the problem that nanogold is gathered due to the fact that the surface of the matrix is modified with nanogold is solved, and the biosensor has a good application prospect. Therefore, high-sensitivity detection is realized, and the method has extraordinary significance in the whole LSPR field.

The present invention discloses an integrated fiber optic biosensor for microdialysis sampling and rapid separation, and its preparation method. The sensor comprises a broadband light source, a transmission optical path, a circulator, a demodulator, a dialysis membrane, a microdialysis channel, an optofluidic electrophoresis channel, an inlet, an outlet, a microinjection pump, and an electrophoresis apparatus. The preparation method includes the following steps: drawing a microstructured optical fiber with a dual-channel structure and two fiber cores; constructing a microdialysis microfluidic circuit and integrating the dialysis membrane within the optical fiber; opening holes perpendicular to the optical fiber in the microdialysis channel and the optofluidic electrophoresis channel to enable the circulation of dialysate and the encapsulation of microelectrodes; fabricating a dual FBG structure within the two fiber cores; and finally coupling the optical fibers to form a closed optical path. This invention addresses the bottleneck problem of existing fiber optic biosensors that rely on off-fiber sample pretreatment, enabling the integrated collection, separation, and detection of complex samples from living tissues, blood vessels, and other locations, thereby promoting the medical application of fiber optic in vivo / online biosensors.

A fiber optic biosensor and detection system includes a transmission fiber and a metal sensing membrane with a nanopore array. The metal sensing membrane is configured to receive incident light from the transmission fiber and reflect the light back into the transmission fiber for detection. This fiber optic biosensor offers advantages such as compactness and portability.

Disclosed is a method for detecting breast cancer biomarkers, which can be applied in cancer diagnosis and monitoring the effectiveness of cancer therapy. This fiber-optic biosensor-based approach employs advanced photonic techniques to identify specific biomarkers with high sensitivity and specificity. The technical result is achieved through a system including a fabricated and biologically functionalized fiber-optic biosensor, immersed in a controlled fluidic environment that mimics in situ conditions. The biosensor is integrated into a commercially available catheter, which is placed within a flow-through tube containing serum. The work focuses on enhancing biosensor performance under simulated blood-like conditions, including optimization of sensor positioning, packaging modifications, and assessment of specificity and sensitivity under varying pressure levels. The aim is to improve the efficiency, accuracy, and reliability of cancer biomarker detection, ultimately enabling earlier and more precise diagnosis for patient benefit.

This application discloses an optical fiber biosensor, a flexible photonic crystal fiber, and a preparation method thereof, relating to the field of optical fiber sensing technology. The preparation method comprises: preparing polyethylene glycol diacrylate, water, and a photocrosslinker according to a set mass ratio to obtain a hydrogel precursor; adding silica microspheres of a set particle size to the hydrogel precursor, performing ultrasonic dispersion and photocrosslinking and curing to obtain an intermediate of the hydrogel photonic crystal fiber; inserting this intermediate into a hydrofluoric acid solution of a set concentration and standing for a first set time to obtain a flexible photonic crystal fiber with a controllable three-dimensional photonic crystal hole structure. By using a flexible photonic crystal fiber as the core of the optical fiber biosensor, this application can achieve high-sensitivity detection, has good stability, and is reusable. This can facilitate the application of optical fiber biosensors in practical situations, broaden the application field of optical fiber sensors, and product preparation.

The present invention provides an optical fiber biosensor, a biological detection device and a biological detection method thereof. The above optical fiber biosensor includes a capillary with an inner cavity and a surface plasmon resonance optical fiber probe. The reflection end of the surface plasmon resonance optical fiber probe completely penetrates the inner cavity and does not contact the inner wall of the capillary. Wherein, the optical fiber biosensor further includes an AC power supply. The first end of the AC power supply is electrically connected to the capillary, and the second end of the AC power supply is electrically connected to the reflection end. The optical fiber biosensor provided by the present invention forms an alternating current electric field between the capillary and the reflection end. Charged particles in the solution to be detected are attracted to the surface plasmon resonance optical fiber probe due to electrophoresis under the action of the alternating current electric field, thereby reducing the detection period of the optical fiber biosensor. At the same time, due to the action of the alternating current electric field, originally non-specifically adsorbed particles are desorbed on the surface of the plasmon resonance optical fiber probe and separated from the charged particles to be detected, thereby improving the detection accuracy.

The invention provides an optical fiber biosensor based on a capillary tube and a preparation method of the optical fiber biosensor, and belongs to the technical field of optical fiber sensing. The optical fiber biosensor based on the capillary tube comprises an optical fiber sensing unit, a peristaltic pump power module 2, a sample pool 3, a transmission optical fiber 5, a computer module 6, a signal acquisition and processing module 7, an optical fiber circulator 8, a light source module 9 and a waste liquid treatment module 10, wherein the optical fiber sensing unit comprises an optical fiber probe 4 and the capillary tube; wherein the transmission direction of optical signals received or reflected by the optical fiber probe 4 is perpendicular to the length direction of the capillary tube, the optical fiber end face of the optical fiber probe 4 is parallel to the length direction of the capillary tube, and the optical fiber end face of the optical fiber probe 4 is not in contact with the capillary tube. The optical fiber biosensor based on the capillary tube is a non-contact optical fiber biosensor, the capillary tube is convenient to disassemble, replace and reuse, and the manufacturing and monitoring cost can be reduced.

The invention discloses an optical fiber biosensor, a flexible photonic crystal fiber and a preparation method thereof, and relates to the technical field of optical fiber sensing, the preparation method comprises the following steps: configuring polyethylene glycol diacrylate, water and a photo-crosslinking agent according to a set mass ratio to obtain a hydrogel precursor; adding silicon dioxide microspheres with a set particle size into the hydrogel precursor, and then carrying out ultrasonic dispersion and photo-crosslinking curing to obtain an intermediate of the hydrogel photonic crystal fiber; and inserting the intermediate into a hydrofluoric acid solution with a set concentration, and standing for a first set time to obtain the flexible photonic crystal fiber with the controllable three-dimensional photonic crystal hole structure. The flexible photonic crystal fiber is adopted as the core of the optical fiber biosensor, high-sensitivity detection can be achieved, the optical fiber biosensor has the advantages of being good in stability and capable of being repeatedly used, then convenience can be provided for application of the optical fiber biosensor in actual occasions, the application field of the optical fiber sensor is widened, and productization preparation of the optical fiber sensor is achieved.

The invention discloses an optical fiber biosensing device based on an SMTMS cascaded SMS inclined throwing structure, and belongs to the technical field of optical fiber sensing. The device comprises a broadband light source, a one-way isolator, a sensing unit, an attenuator, a signal processing unit and an upper computer which are sequentially connected through single-mode optical fibers. The sensing unit is of an SMS side face inclined throwing cascade SMTMS structure. The multi-mode optical fiber side surface oblique throwing increase in the SMS can increase the evanescent field proportion and the surface sensing area so as to improve the sensitivity, and the oblique throwing depth is adjustable. The conical optical fiber in the SMTMS is used for preparing a non-adiabatic cone through a fused biconical taper method, the interference effect of a high-order mode and a fundamental mode is excited, the free spectral range is expanded through the vernier effect of a cascade structure, and sensitivity amplification is achieved. The combination change of external biomolecules causes the change of the refractive index of the optical fiber, the wavelength shift of the interference spectrum is modulated, and the signal processing unit inverts a parameter value by demodulating the spectrum offset. And the detection limit is 1fM when the concentration of the target DNA is in a range of 0-1.0 mu M. The device solves the problems of low detection limit, cross sensitivity and poor environmental adaptability of a traditional sensor, and is suitable for high-precision DNA sensing in monitoring, biomedical treatment and complex environments.

The utility model belongs to the technical field of quantum measurement and optical fiber sensing, and particularly relates to a conical coreless optical fiber biosensor based on quantum measurement. The conical coreless optical fiber biosensor is used for measurement of a photosensitive biological fragile system. The quantum entanglement source used in the utility model adopts a Sagnac interferometer structure, stable optical path configuration is provided, entanglement photon pairs generated through spontaneous parametric down-conversion (SPDC) have high quantum correlation, and a high signal-to-noise ratio can still be maintained under weak light detection. When the concentration of an analyte in a to-be-detected sample changes, the biological sensitive film on the conical surface captures the change of the analyte, so that the refractive index of the conical surface changes, the interference spectrum of the sensor moves, the light intensity at the wavelength position of signal light changes, and the number of signal photons changes; the photon counting of the first single-photon detector changes, and the second-order correlation measurement result of the signal photons and the reference photons on the coincidence counter changes, that is, the coincidence counting value changes. And establishing a proportional relation between the coincidence count value and the concentration of the to-be-measured sample, and measuring the concentration of the to-be-measured sample by monitoring the change of the coincidence count value.

The invention discloses an optical fiber biosensor, which is prepared by coating the surface of an amino-functionalized waist-tapered optical fiber sensor with a graphene oxide layer and adsorbing an aptamer NE-AP or an aptamer LF-AP on the surface of the graphene oxide layer. The aptamer NE-AP can be specifically combined with the elastinase ELANE; the aptamer LF-AP can be specifically combined with the lactoferrin LF. After the optical fiber biosensor captures ELANE and LF proteins, the roughness and thickness of the surface of the optical fiber at the waist-cone-shaped part are changed, when light penetrates through the waist-cone-shaped part covered by biomolecules, the effective refractive index of the surface of the optical fiber biosensor is changed due to the fact that the diameter is gradually reduced and an evanescent field outside a core is enhanced, and the optical fiber biosensor can be used for detecting the optical fiber in the waist-cone-shaped part. Therefore, red shift occurs according to a transmitted spectrum induced after aptamer-protein combination is tracked, and high-sensitivity and low-detection-limit detection on the activity of the neutrophil is realized. The method has the characteristics of high sensitivity and low detection limit.

The invention provides an optical fiber DNA sensor prepared by combining a molybdenum disulfide material and a detection method, a micro-fluidic chip with a tip coupled bubble structure is provided, the micro-fluidic chip comprises an elastic material chip and a glass sheet which are bonded, the plane of the glass sheet covers the plane projection part of the elastic material chip, and the plane projection part of the elastic material chip covers the plane projection part of the glass sheet. One plane of the elastic material chip is used as a bonding plane to be bonded with the glass sheet, a groove penetrating through the length direction of the elastic material chip is formed in the bonding plane, and the groove is used for placing the optical fiber biosensor in the length direction and is filled with liquid acting on the optical fiber biosensor; the groove comprises a liquid channel, a first extension area, a second extension area and a plurality of tip coupling bubble structures, the first extension area and the second extension area are located on the two sides of the liquid channel in the length direction respectively, and the tip coupling bubble structures are located on one side of the liquid channel in the width direction.

The invention relates to the technical field related to molecular diagnosis, and particularly discloses a coding gene, a recombinant plasmid, a cell line, a recombinant protein, a capture sensor and a method for detecting a mycoplasma pneumoniae antibody based on a biological membrane interference technology. The nucleotide sequence of the coding gene is as shown in SEQ ID No: 1. The amino acid sequence of the recombinant protein is as shown in SEQ ID No.2. The capture sensor comprises an optical fiber biosensor, and the optical fiber end face of the optical fiber biosensor is combined with the recombinant protein of the chlamydia pneumoniae. According to the recombinant protein encoded by the encoding gene, and the mycoplasma pneumoniae antibody is detected by using the recombinant protein antigen, so that the detection limit of the mycoplasma pneumoniae antibody is greatly improved, and a false positive result is avoided.

The application discloses a SpyCatcher immobilized mutant SpyFixer and application thereof. The mutant is obtained by introducing an A77E mutation site based on SpyDock. The mutant is respectively immobilized on a fiber biosensor, a hydrophilic epoxy agarose resin and a hydrophobic epoxy acrylate resin to obtain different carriers modified by SpyFixer, and then target proteins containing SpyTag are respectively immobilized on the different carriers modified by SpyFixer through Spy chemistry, and are used for protein interaction detection, immune detection, purification and immobilization. The mutant can be effectively immobilized on various types of carriers, and the immobilization efficiency and the binding activity after immobilization are superior to those of SpyCatcher and SpyCatcher003, and the application range is wider. Moreover, the proteins immobilized on the carriers modified by SpyFixer can be repeatedly used, and have potential industrial utilization value.

This invention provides a capillary-based fiber optic biosensor and its fabrication method, belonging to the field of fiber optic sensing technology. The capillary-based fiber optic biosensor of this invention includes a fiber optic sensing unit, a peristaltic pump power module 2, a sample cell 3, a transmission fiber optic cable 5, a computer module 6, a signal acquisition and processing module 7, a fiber optic circulator 8, a light source module 9, and a waste liquid treatment module 10. The fiber optic sensing unit includes a fiber optic probe 4 and a capillary; wherein the direction of light signal transmission received or reflected by the fiber optic probe 4 is perpendicular to the length direction of the capillary, the fiber end face of the fiber optic probe 4 is parallel to the length direction of the capillary, and the fiber end face of the fiber optic probe 4 does not contact the capillary. The capillary-based fiber optic biosensor provided by this invention is a non-contact fiber optic biosensor, which facilitates disassembly and replacement of the capillary for reuse, thus helping to reduce manufacturing and monitoring costs.