12 results about "Fiber optic biosensor" patented technology

The invention relates to the field of biochemical sensing, in particular to a nucleic acid aptamer biosensor, an optical fiber sensor and cell detection application. The nucleic acid aptamer biosensor comprises a matrix, a connecting structure and TDN-coated AuNP, the matrix and the TDN-coated AuNP are combined through the connecting structure, and the connecting structure is an amino group, PolyA or a functionalized complementary chain. Through a special tetrahedral nanogold structure, the problem that nanogold is gathered due to the fact that the surface of the matrix is modified with nanogold is solved, and the biosensor has a good application prospect. Therefore, high-sensitivity detection is realized, and the method has extraordinary significance in the whole LSPR field.

The present invention discloses an integrated fiber optic biosensor for microdialysis sampling and rapid separation, and its preparation method. The sensor comprises a broadband light source, a transmission optical path, a circulator, a demodulator, a dialysis membrane, a microdialysis channel, an optofluidic electrophoresis channel, an inlet, an outlet, a microinjection pump, and an electrophoresis apparatus. The preparation method includes the following steps: drawing a microstructured optical fiber with a dual-channel structure and two fiber cores; constructing a microdialysis microfluidic circuit and integrating the dialysis membrane within the optical fiber; opening holes perpendicular to the optical fiber in the microdialysis channel and the optofluidic electrophoresis channel to enable the circulation of dialysate and the encapsulation of microelectrodes; fabricating a dual FBG structure within the two fiber cores; and finally coupling the optical fibers to form a closed optical path. This invention addresses the bottleneck problem of existing fiber optic biosensors that rely on off-fiber sample pretreatment, enabling the integrated collection, separation, and detection of complex samples from living tissues, blood vessels, and other locations, thereby promoting the medical application of fiber optic in vivo / online biosensors.

A fiber optic biosensor and detection system includes a transmission fiber and a metal sensing membrane with a nanopore array. The metal sensing membrane is configured to receive incident light from the transmission fiber and reflect the light back into the transmission fiber for detection. This fiber optic biosensor offers advantages such as compactness and portability.

Disclosed is a method for detecting breast cancer biomarkers, which can be applied in cancer diagnosis and monitoring the effectiveness of cancer therapy. This fiber-optic biosensor-based approach employs advanced photonic techniques to identify specific biomarkers with high sensitivity and specificity. The technical result is achieved through a system including a fabricated and biologically functionalized fiber-optic biosensor, immersed in a controlled fluidic environment that mimics in situ conditions. The biosensor is integrated into a commercially available catheter, which is placed within a flow-through tube containing serum. The work focuses on enhancing biosensor performance under simulated blood-like conditions, including optimization of sensor positioning, packaging modifications, and assessment of specificity and sensitivity under varying pressure levels. The aim is to improve the efficiency, accuracy, and reliability of cancer biomarker detection, ultimately enabling earlier and more precise diagnosis for patient benefit.

This application discloses an optical fiber biosensor, a flexible photonic crystal fiber, and a preparation method thereof, relating to the field of optical fiber sensing technology. The preparation method comprises: preparing polyethylene glycol diacrylate, water, and a photocrosslinker according to a set mass ratio to obtain a hydrogel precursor; adding silica microspheres of a set particle size to the hydrogel precursor, performing ultrasonic dispersion and photocrosslinking and curing to obtain an intermediate of the hydrogel photonic crystal fiber; inserting this intermediate into a hydrofluoric acid solution of a set concentration and standing for a first set time to obtain a flexible photonic crystal fiber with a controllable three-dimensional photonic crystal hole structure. By using a flexible photonic crystal fiber as the core of the optical fiber biosensor, this application can achieve high-sensitivity detection, has good stability, and is reusable. This can facilitate the application of optical fiber biosensors in practical situations, broaden the application field of optical fiber sensors, and product preparation.

The invention provides an optical fiber biosensor based on a capillary tube and a preparation method of the optical fiber biosensor, and belongs to the technical field of optical fiber sensing. The optical fiber biosensor based on the capillary tube comprises an optical fiber sensing unit, a peristaltic pump power module 2, a sample pool 3, a transmission optical fiber 5, a computer module 6, a signal acquisition and processing module 7, an optical fiber circulator 8, a light source module 9 and a waste liquid treatment module 10, wherein the optical fiber sensing unit comprises an optical fiber probe 4 and the capillary tube; wherein the transmission direction of optical signals received or reflected by the optical fiber probe 4 is perpendicular to the length direction of the capillary tube, the optical fiber end face of the optical fiber probe 4 is parallel to the length direction of the capillary tube, and the optical fiber end face of the optical fiber probe 4 is not in contact with the capillary tube. The optical fiber biosensor based on the capillary tube is a non-contact optical fiber biosensor, the capillary tube is convenient to disassemble, replace and reuse, and the manufacturing and monitoring cost can be reduced.

The invention discloses an optical fiber biosensing device based on an SMTMS cascaded SMS inclined throwing structure, and belongs to the technical field of optical fiber sensing. The device comprises a broadband light source, a one-way isolator, a sensing unit, an attenuator, a signal processing unit and an upper computer which are sequentially connected through single-mode optical fibers. The sensing unit is of an SMS side face inclined throwing cascade SMTMS structure. The multi-mode optical fiber side surface oblique throwing increase in the SMS can increase the evanescent field proportion and the surface sensing area so as to improve the sensitivity, and the oblique throwing depth is adjustable. The conical optical fiber in the SMTMS is used for preparing a non-adiabatic cone through a fused biconical taper method, the interference effect of a high-order mode and a fundamental mode is excited, the free spectral range is expanded through the vernier effect of a cascade structure, and sensitivity amplification is achieved. The combination change of external biomolecules causes the change of the refractive index of the optical fiber, the wavelength shift of the interference spectrum is modulated, and the signal processing unit inverts a parameter value by demodulating the spectrum offset. And the detection limit is 1fM when the concentration of the target DNA is in a range of 0-1.0 mu M. The device solves the problems of low detection limit, cross sensitivity and poor environmental adaptability of a traditional sensor, and is suitable for high-precision DNA sensing in monitoring, biomedical treatment and complex environments.

The invention discloses an optical fiber biosensor, which is prepared by coating the surface of an amino-functionalized waist-tapered optical fiber sensor with a graphene oxide layer and adsorbing an aptamer NE-AP or an aptamer LF-AP on the surface of the graphene oxide layer. The aptamer NE-AP can be specifically combined with the elastinase ELANE; the aptamer LF-AP can be specifically combined with the lactoferrin LF. After the optical fiber biosensor captures ELANE and LF proteins, the roughness and thickness of the surface of the optical fiber at the waist-cone-shaped part are changed, when light penetrates through the waist-cone-shaped part covered by biomolecules, the effective refractive index of the surface of the optical fiber biosensor is changed due to the fact that the diameter is gradually reduced and an evanescent field outside a core is enhanced, and the optical fiber biosensor can be used for detecting the optical fiber in the waist-cone-shaped part. Therefore, red shift occurs according to a transmitted spectrum induced after aptamer-protein combination is tracked, and high-sensitivity and low-detection-limit detection on the activity of the neutrophil is realized. The method has the characteristics of high sensitivity and low detection limit.

The invention relates to the technical field related to molecular diagnosis, and particularly discloses a coding gene, a recombinant plasmid, a cell line, a recombinant protein, a capture sensor and a method for detecting a mycoplasma pneumoniae antibody based on a biological membrane interference technology. The nucleotide sequence of the coding gene is as shown in SEQ ID No: 1. The amino acid sequence of the recombinant protein is as shown in SEQ ID No.2. The capture sensor comprises an optical fiber biosensor, and the optical fiber end face of the optical fiber biosensor is combined with the recombinant protein of the chlamydia pneumoniae. According to the recombinant protein encoded by the encoding gene, and the mycoplasma pneumoniae antibody is detected by using the recombinant protein antigen, so that the detection limit of the mycoplasma pneumoniae antibody is greatly improved, and a false positive result is avoided.

The application discloses a SpyCatcher immobilized mutant SpyFixer and application thereof. The mutant is obtained by introducing an A77E mutation site based on SpyDock. The mutant is respectively immobilized on a fiber biosensor, a hydrophilic epoxy agarose resin and a hydrophobic epoxy acrylate resin to obtain different carriers modified by SpyFixer, and then target proteins containing SpyTag are respectively immobilized on the different carriers modified by SpyFixer through Spy chemistry, and are used for protein interaction detection, immune detection, purification and immobilization. The mutant can be effectively immobilized on various types of carriers, and the immobilization efficiency and the binding activity after immobilization are superior to those of SpyCatcher and SpyCatcher003, and the application range is wider. Moreover, the proteins immobilized on the carriers modified by SpyFixer can be repeatedly used, and have potential industrial utilization value.

This invention provides a capillary-based fiber optic biosensor and its fabrication method, belonging to the field of fiber optic sensing technology. The capillary-based fiber optic biosensor of this invention includes a fiber optic sensing unit, a peristaltic pump power module 2, a sample cell 3, a transmission fiber optic cable 5, a computer module 6, a signal acquisition and processing module 7, a fiber optic circulator 8, a light source module 9, and a waste liquid treatment module 10. The fiber optic sensing unit includes a fiber optic probe 4 and a capillary; wherein the direction of light signal transmission received or reflected by the fiber optic probe 4 is perpendicular to the length direction of the capillary, the fiber end face of the fiber optic probe 4 is parallel to the length direction of the capillary, and the fiber end face of the fiber optic probe 4 does not contact the capillary. The capillary-based fiber optic biosensor provided by this invention is a non-contact fiber optic biosensor, which facilitates disassembly and replacement of the capillary for reuse, thus helping to reduce manufacturing and monitoring costs.