3 results about "Fiber optic biosensor" patented technology

The application discloses a SpyCatcher immobilized mutant SpyFixer and application thereof. The mutant is obtained by introducing an A77E mutation site based on SpyDock. The mutant is respectively immobilized on a fiber biosensor, a hydrophilic epoxy agarose resin and a hydrophobic epoxy acrylate resin to obtain different carriers modified by SpyFixer, and then target proteins containing SpyTag are respectively immobilized on the different carriers modified by SpyFixer through Spy chemistry, and are used for protein interaction detection, immune detection, purification and immobilization. The mutant can be effectively immobilized on various types of carriers, and the immobilization efficiency and the binding activity after immobilization are superior to those of SpyCatcher and SpyCatcher003, and the application range is wider. Moreover, the proteins immobilized on the carriers modified by SpyFixer can be repeatedly used, and have potential industrial utilization value.

This invention provides a capillary-based fiber optic biosensor and its fabrication method, belonging to the field of fiber optic sensing technology. The capillary-based fiber optic biosensor of this invention includes a fiber optic sensing unit, a peristaltic pump power module 2, a sample cell 3, a transmission fiber optic cable 5, a computer module 6, a signal acquisition and processing module 7, a fiber optic circulator 8, a light source module 9, and a waste liquid treatment module 10. The fiber optic sensing unit includes a fiber optic probe 4 and a capillary; wherein the direction of light signal transmission received or reflected by the fiber optic probe 4 is perpendicular to the length direction of the capillary, the fiber end face of the fiber optic probe 4 is parallel to the length direction of the capillary, and the fiber end face of the fiber optic probe 4 does not contact the capillary. The capillary-based fiber optic biosensor provided by this invention is a non-contact fiber optic biosensor, which facilitates disassembly and replacement of the capillary for reuse, thus helping to reduce manufacturing and monitoring costs.