40 results about "Fiber optic biosensor" patented technology

The present invention relates to a surface plasmon resonance detector, that is portable and easy to operate, and its optical-fiber biosensor unit can be readily replaced. The SPR detector of the present invention comprises: a light source; an optical-fiber biosensor unit having a well, a coating layer, and a core layer; an optical detector; a plurality of optical fibers connecting with the light source, the optical-fiber biosensor unit and the optical detector; and a calculation and display unit connecting with the optical detector, wherein the optical detector receives the optical signals from the optical detector and display the calculation results thereof. Besides, the SPR detector of the present invention has high sensitivity and is able to identify species of trace biomolecules.

The invention provides a plasma resonant type optical fiber biosensor based on annular core wave guide, which consists of a broad band light source, an optical fiber coupler, an optical fiber probe, a detector, an optical spectrometer and a signal processor through being connected, wherein the optical fiber coupler is formed by single mode optical fiber and annular core optical fiber through being coupled, the single mode optical fiber of the optical fiber coupler is connected with the output of the broad band light source, and the annular core optical fiber is connected with the optical fiber probe. The optical fiber probe belongs to an optical fiber probe manufactured by a method comprising the following steps: grinding the annular core single mode optical fiber into a frustum with the designed angle and the designed height; using vacuum evaporation film plating equipment for plating a golden film for depositing a layer of golden film on the surface of the frustum; and carrying out secondary film plating for forming a reflecting film on the end surface of the optical fiber. The invention has the small size, reduces mechanical parts of the biosensor, is suitable for remote distance detection, is convenient for the instrument integration, and can detect the change of the external environment in real time. The invention has higher optical coupling efficiency than the traditional single mode optical fiber. The manufacture of the sensing probe is convenient, and the repetitiveness is good. The detection sensitivity is high, and the test results are stable and reliable.

The invention relates to a thin-film material for an optical fiber biosensor probe and a preparation method thereof. The material has catalytic characteristic and photosensitivity and is made from Fe3O4/SiO2 magnetic nanocomposite particles, bioactive enzyme, fluorescent material and sol-gel. The bioactive enzyme is fixed on the Fe3O4/SiO2 magnetic nanocomposite particles and the fixed bioactive enzyme and the fluorescent material are embedded in a sol-gel grid. The preparation method comprises the following steps: a Fe3O4/SiO2 magnetic nanocomposite particle carrier is synthesized; the carrier is activated by glutaraldehyde or by glutaraldehyde, bovine serum albumin and glutaraldehyde in sequence and then has a crosslinking reaction with the bioactive enzyme to fix the enzyme; the Fe3O4/SiO2 magnetic nanocomposite particles fixed with the bioactive enzyme are dripped on the surface of a treated glass substrate after being mixed with the sol-gel liquid blended with a Ru(bpy)3Cl2 fluorescent indicator; after cleaning, drying and aging, a sol-gel optical bio-composite sensing film embedding the bioactive enzyme and the fluorescent indicator is obtained. The optical fiber biosensor probe made from the thin-film material is especially applicable to the detection of blood sugar, blood cholesterol and the like.

A biconically tapered optical fiber serves as a biosensor at all optical wavelengths of interest ranging from UV to far IR at subfemtogram per mL sensitivity. The biconically tapered sensor detects biomaterials such as pathogenic species, proteins and DNA and others biological analytes. Although it uses the principles of evanescent fields, absorption, adsorption, fluorescence capture and retransmission through the fiber, the detection at the sub-nanogram per mL level is achieved primarily by adsorption or a surface activity due to a refractive index change. The geometry of the biconically tapered optical sensor affects its performance. The sensing modality is achieved in situ with a source connected at one end of a tapered fiber and a suitable detector at the other end. The tapered region is optionally immobilized with a recognition molecule such as an antibody to the target antigen or a complementary DNA strand. The sample is brought in contact with the tapered region either in batch mode or in a flow mode.

A method for biosensing that includes passing, via convective flow, a sample believed to contain one or more target biomarkers through a microfluidic channel and over the surface of an optical waveguide that has been prepared to bind the one or more target biomarkers, and sensing for an emission output from the optical waveguide at a wavelength that is characteristic of the binding of the target biomarker. A biosensor device that includes a module defining at least one microfluidic channel, an optical waveguide exposed along at least a portion of its length to fluid flow within the microfluidic channel, where a surface of the optical waveguide being prepared to bind a target biomarker, and an excitation source to couple an excitation wavelength of light into the optical waveguide. The device also includes a sensor for detecting emission light from the optical waveguide at an emission wavelength characteristic of binding of the target biomarker.

A fluorescent fiber-optic biosensor system using ultrasonic concentration of particles and cells for the detection of Salmonella typhimurium. A biosensor test chamber serves as an ultrasonic standing-wave cell that allows microspheres or cells to be concentrated in parallel layers or in a column along the axis of the cell. A fiber probe along the axis delivers laser excitation to fluorescent-labeled antibodies of Salmonella and collects the fluorescent signal. The Salmonella-antibody complexes are moved acoustically to the axis of the cell, increasing the fluorescent signal. Alternatively, the Salmonella-labelled antibody complexes attach to unlabeled antibodies that have been immobilized on the surface of polystyrene microspheres. This entire structure can be manipulated acoustically and the increase in the fluorescent signal, which can be an order of magnitude, indicates the presence of Salmonella.

An optical waveguide sensing method and device in which a waveguide layer receives an optical signal and propagates the optical signal in accordance with a predetermined optical waveguide propagation mode. A testing medium surface in communication with the waveguide layer is responsive to a testing medium for modifying at least one characteristic of the propagated optical signal in relation to a given parameter of the testing medium. In this manner, the modified characteristic of the propagated optical signal can be measured in view of determining the given parameter of the testing medium.

Provided is a micro-nano fiber biosensor which relates to the optics field and concretely relates to a biosensor. The micro-nano fiber biosensor aims to solve problems of complex structure, poor stability and low accuracy of real-time in vivo measurement devices for Brillouin spectra of living bodies. Laser emitted from a laser device of the micro-nano fiber biosensor goes into a coupler, and is divided into a number one light beam and a number two light beam by the coupler. The number one light beam is modulated by a single-side band modulator, and goes into a number one light input end of a circulator. The intensity of the number two light beam is modulated by an electrooptical modulator. The number two light beam is amplified by an erbium-doped optical fiber amplifier and goes into a number two light input end of the circulator. Light emitted from a light output end of the circulator goes into a light-sensing end of a photoelectric detector. Electrical signals of the photoelectric detector are output to an oscilloscope by a lock-in amplifier. The micro-nano biosensor is suitable for real-time in vivo measurement for Brillouin spectra of living bodies.

In order to solve the problem of low sensitivity of existing fiber optic SPR biosensors, the invention provides a sensitized fiber optic SPR sensor based on graphene oxide and gold nanorods. Accordingto the sensitized fiber optic SPR sensor based on the graphene oxide and the gold nanorods, the plasmon coupling effect between a plasma wave on the surface of a gold film and a local surface plasmawave on the surface of the gold nanorods is utilized to enhance the electric field strength. At the same time, the charge transfer between the gold film and the gold nanorods can be promoted by the graphene oxide, so that interactions between a surface plasma wave and a substance to be detected is enhanced, and the detection sensitivity is improved. In addition, the graphene oxide has excellent bio-sensing characteristics, biocompatibility, and a large specific surface area, can better measure biomass and chemical quantities. Compared to an ordinary fiber optic SPR sensor, the sensitized fiberoptic SPR sensor based on the graphene oxide and the gold nanorods has higher sensing sensitivity and chemical stability, real-time monitoring can be performed, the structure of the sensor is compact, thereby being able to widely applied in the fields of physics, chemistry, biology, medical care, food safety and the like.

The invention relates to a multi-channel evanescent wave all-optical fiber biosensor. The biosensor is characterized in that it comprises a flow sample injection system, an optical system and a control circuit and signal processing system; laser emitted by a semiconductor laser unit is collimated and then divided into a plurality of light beams, each light beam is emitted by a corresponding all-optical fiber system to a corresponding sample pool, each optical fiber probe produces fluorescence after excitation, the generated fluorescence signals are collected and filtered by all-optical fiber systems and are then emitted to a photoelectric detector group, the photoelectric detector group converts the collected fluorescence signals of all the optical fiber probes' surfaces into current signals, multi-channel current signals are processed by a micro-signal simulation preamplifier and a multi-channel lock-in amplifier and then sent to a computer, thus realizing simultaneous quantitative analysis of a sample's multiple indexes; and the computer is also connected to a cooperative control module through a parallel processing module, the parallel processing module is also connected to the semiconductor laser unit through a pulse signal generator, and the cooperative control module is in parallel connection with a peristaltic pump and a ten-position valve.

The invention discloses a whispering-gallery-mode fiber optic biosensor which is provided with at least one optical fiber and at least one micro-resonator. The whispering-gallery-mode fiber optic biosensor can couple the light in the optical fiber to the micro-resonator and excite the whispering gallery mode, the fiber optic biosensor is also provided with a substrate which can be inserted in an object to be measured, the optical fiber is Y-shaped, one branch of the Y-shaped optical fiber is a light incidence end which can be connected with a light source, the other branch is a light emergence end which can be connected with a detector, the lower end of the Y-shaped optical fiber is fixed on the substrate and the tail end is fixedly provided with a reflector, and the micro-resonator is fixed on the substrate and coupled with the lower end of the Y-shaped optical fiber. When the fiber optic biosensor is in use, the light source and the detecting instrument are arranged at the same side of the micro-resonator, thereby being beneficial for detecting and moving and realizing detection for the analyte to be measured in real time without marking the detected object. The substrate of the fiber optic biosensor also can be inserted in the analyte to be measured, therefore the detection is convenient, safe and stable, and outdoor detection can be realized as well. Meanwhile, the technical scheme leads the light wave to be coupled twice in the micro-resonator, thereby further improving the precision and sensitivity of the whispering-gallery-mode sensor.

The invention relates to a dual-channel optical fiber SPR sensor. The dual-channel SPR sensor comprises a reference channel and a sensing channel; the reference channel serves as a blank control reference channel and detects a wavelength shift quantity caused by non-specific adsorption; and the sensing channel improves the detection sensitivity of the sensor by enhancing the intensity of a local electric field through utilizing a coupling effect between a gold film and gold nanoparticles, and improves the immobilization efficiency of an antibody by utilizing a graphene oxide thin film. The twochannels in the dual-channel optical fiber SPR sensor use different metal films respectively, so that two resonance valleys are generated, double-channel sensing is realized, and the unique advantages of eliminating a measuring error caused by the non-specific adsorption and being insensitive to the temperature are achieved; and the two SPR resonance valleys of the two channels are separated fromeach other, so that the SPR resonance valleys are prevented from generating the adverse effects mutually in the detection process, and biological sensing is better facilitated. According to the sensor, the problems that the sensor is low in sensitivity and easy to influence by liquid refractive index change and temperature fluctuation in the prior art are effectively solved.

The invention discloses a method for fast detecting beta-lactamase contained in milk products by an optical fiber biosensor, belonging to the field of the optical fiber biosensor and comprising an optical system, an electronic system, a data collection system, a control and processing system, optical fibers and a sensor, wherein optical fiber material is polystyrene; the sensor is provided with a sensitive film, the sensitive film contains a beta-lactamase monoclonal antibody fixed by glutaraldehyde and marked by Cy5, and the monoclonal antibody is prepared by taking the beta-lactamase as an antigenic and immunized mouse, fusing cells, and the like. The invention has the advantages of high sensitivity, electromagnetic radiation resistance, electronic frequency interference resistance, corrosion resistance, small instrument volume, portability, few needed detection sample, rapid response time, easy operation, low cost, and the like and can be used for detecting the beta-lactamase contained in the milk products, such as liquid milk, powdered milk, solid samples, and the like.

A sensor for sensing and monitoring a property associated with transformation of a biochemical analyte by a micro-organism, the sensor comprising a glass permeable coating applied to an unclad portion (14) of a fibre optic member (13). The coating has a transformable precursor impregnated into it, the precursor being specifically metabolisable by one or more targeted organisms. When the sensor is placed in contact with a sample in a container (15) contacting an active targeted micro-organisms, the precursor is transformed by the micro-organisms to produce a spectroscopically detectable indicator of the property of the analyte. Spectroscopic information may be analysed by a computer program to provide an overall index of microbiological activity for the targeted micro-organism. The invention extends to a method of producing a sensor and a method of identifying the presence of a targeted micro-organism.

The invention discloses a staphylococcus aureus detection method. The method comprises the following steps: preparing staphylococcus aureus culture solution I; adding a sample to be detected in the culture solution I for enrichment culture to prepare culture solution II for standby use; preparing an optical fiber biosensor: enabling protein specific antibody I of staphylococcus aureus A to be immobilized on the optical fiber biosensor under the hydrophobic forces and the intermolecular forces or under the action of covalent bonds; preparing blank detection liquid and liquid to be detected, wherein the blank detection liquid comprises the culture solution I and buffer solution, the liquid to be detected comprises the culture solution II and buffer solution; immersing the prepared optical fiber biosensor into the blank detection liquid for equalization treatment, and then immersing into the liquid to be detected for detection. Compared with the detection method in the prior art, the staphylococcus aureus detection method has the advantages of real-time detection, short detection time, convenience in operation and the like and has good application prospect.

The invention relates to a biological detection instrument, in particular to a biological fast detection system for quickly detecting various biological targets, which comprises an evanescent wave optical fiber biological sensor and a recognition biological molecule solution with fluorescent labels, wherein the recognition biological molecule solution with fluorescent labels is provided with conversion nanometer crystal labels. The biological fast detection system improves the stability of fluorescent detection signals and the signal/noise ratio of the detection fluorescent signal of the evanescent wave optical fiber biological sensor.

A multiplex fiber optic biosensor including an optical fiber, a plurality of noble metal nanoparticle layers, a plurality of light sources and a light source function generator is disclosed. The optical fiber includes a plurality of sensing regions which are unclad regions of the optical fiber so that the fiber core is exposed, wherein the noble metal nanoparticle layers are set in each sensing regions. The light sources emit light with different wavelengths, and the noble metal nanoparticle layers absorb the lights with different wavelengths, respectively. The light sources emit the lights in different timing sequences or different carrier frequencies, wherein when the lights propagate along the optical fiber in accordance with the different timing sequences or the different carrier frequencies, a detection unit detects particle plasmon resonance signals produced by interactions between the different noble metal nanoparticle layers and the corresponding analytes.

The invention discloses a portable multi-sample detection fiber optic biosensor. The fiber optic biosensor comprises a light source module, a light splitting module, a fiber optic probe, a filter module and a mobile device. A laser signal is transmitted to the fiber optic probe through the light splitting module and excites the quantum dot of the probe to produce a fluorescence signal, then the signal is reversely transmitted to the filter module, then the signal light is detected through a camera of the mobile device and the detection of different samples is realized through separation basedon an algorithm. The imaging detection and image processing are used in the fiber optic biosensor. The fiber optic biosensor has a compact structure, has a small size, is portable, greatly improves the utilization efficiency of laser and fluorescence and can be widely used in the fields of food safety, biomedicine, environmental monitoring and military.

The invention discloses an optical fiber biosensor, the surface of the optical fiber sensor is simultaneously modified with biological recognition molecules and a chemiluminescence substrate, and the optical fiber sensor can be applied to homogeneous chemiluminescence biological detection. The invention also discloses a homogeneous chemiluminescence biological detection method, in the method, the optical fiber biosensor is used, the chemiluminescence substrate concentration and reaction speed are controlled at the same time, and specific and non-specific detection signals are distinguished from the generation time of a luminescence signal, so that the interference of the non-specific signal is avoided; and the accuracy and the sensitivity of homogeneous detection are improved.

The invention relates to an optical fiber biological probe for the evanescent wave optical fiber biological sensor in immunity test, in particular to an optical fiber biological probe which surface is directionally fixed with antibodies and a preparation method thereof. The biological probe is composed of an optical fiber and a capture antibody molecule layer fixed on the surface of the optical fiber, which is characterized in that a protein G molecule layer is arranged between the optical fiber and the capture antibody molecule layer. The preparation method comprises: immersing an clean optical fiber into a protein G solution, to obtain the saturated adsorption layer of the protein G, immersing into the capture antibody molecule solution, to combine the capture antibody molecules via the proteins G until saturation, to obtain the directional fixed antibody optical fiber biological probe. The optical fiber biological probe overcomes the defect such as reduced biological activity of the antibody caused by directly fixing antibodies on the surface of the fiber core, to reduce the spatial resistance of the antibody molecules, effectively reduces the non-specific adsorption of the surface, and improves the analysis sensitivity of the detection antigens.