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Biological rapid detecting system

A detection system and fast technology, applied in the field of high-sensitivity detection systems, can solve the problems of poor stability of biological fluorescence signals, wide fluorescence spectrum of fluorescent dyes, and narrow excitation spectrum lines, so as to improve detection sensitivity and stability, and improve signal-to-noise ratio. , Emitting the effect of narrow spectral lines

Inactive Publication Date: 2009-03-18
CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

[0006] The purpose of the present invention is to overcome the poor stability of the bioluminescent signal detected by the optical fiber biosensor and the poor stability of the biomolecular or The shortcomings of autofluorescence produced by the biological environment provide a high-sensitivity biological rapid detection system for the detection of target biomolecules

Method used

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  • Biological rapid detecting system

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Experimental program
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Effect test

Embodiment 1

[0019] (1) Preparation of optical fiber bioprobe.

[0020] a. surface hydrophilic polarization treatment: the fiber core surface of the optical fiber biological probe is first cleaned in cleaning solution 1 for 30 minutes, then cleaned in cleaning solution 2 for 30 minutes, then boiled in cleaning solution 3 for 10 minutes, A polarized layer with hydrophilicity is prepared on the surface-matrixed fiber core.

[0021] Wherein the cleaning solution 1 is: concentrated HCl: ethanol = 1: 1 (V / V);

[0022] Cleaning solution 2 is: concentrated sulfuric acid;

[0023] Cleaning liquid 3 is: ultrapure water.

[0024] b. Preparation of capture molecular layer: put the above-mentioned surface hydrophilized fiber core into IgG protein solution (1mg / ml), so that the protein molecules are naturally adsorbed on the surface of the fiber core for 30 minutes, and then washed, that is Fiber optic bioprobes for detection are formed.

[0025] (2) Preparation of the fluorescently labeled recogni...

Embodiment 2

[0030] (1) The optical fiber bioprobe containing the capture molecular layer IgG was prepared completely according to the process of Example 1.

[0031] (2) Take 2mL concentration as 10 -7 Amino Y of M 2 o 3 : Er, Yb UCP nanocrystal solution, add 20μL 0.05M glutaraldehyde solution to the UCP nanocrystal solution, stir for 30-60 minutes; then add the same concentration of anti-IgG antibody to the above mixture to obtain UCP Nanocrystalline Labeled Biomolecular Solutions for Recognition.

[0032] NaYF can also be prepared according to the process of Example 1 4 : Yb, Ho, ZnS: Yb, Eu or ZnCl 2 : Yb, Tm and other UCP nanocrystal-labeled recognition molecule solution.

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Abstract

The invention relates to a biological detection instrument, in particular to a biological fast detection system for quickly detecting various biological targets, which comprises an evanescent wave optical fiber biological sensor and a recognition biological molecule solution with fluorescent labels, wherein the recognition biological molecule solution with fluorescent labels is provided with conversion nanometer crystal labels. The biological fast detection system improves the stability of fluorescent detection signals and the signal / noise ratio of the detection fluorescent signal of the evanescent wave optical fiber biological sensor.

Description

technical field [0001] The invention relates to a biological detection device that can be widely used in fields such as molecular biology, biomedicine, food inspection, biological warfare agents, and environmental monitoring, especially a device that can detect various biological targets, such as bacteria, biological warfare agent molecules, A highly sensitive detection system for rapid detection of biomolecules such as viruses, deoxyribonucleic acid (DNA), peptides, antigens and antibodies. Background technique [0002] At present, the widely used biological detection system based on evanescent wave optical fiber biosensor is to use the evanescent wave excitation generated during the transmission of light waves in the optical fiber in the form of total reflection to bind to the surface of the optical fiber probe with a bioaffinity reaction. Fluorescent dyes for biomolecules. A fiber optic probe immobilized on the surface to capture biomolecules is placed in the sample to b...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N21/64
Inventor 孔祥贵张友林曾庆辉孙雅娟刘晓敏
Owner CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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