A kind of rapid diagnostic test paper and preparation method thereof

A rapid diagnosis and test strip technology, applied in biological testing, material inspection products, measuring devices, etc., can solve the problems of unstable signal, poor anti-interference ability, low sensitivity, etc., to improve specificity, improve stability, and improve signal Effects of stability and anti-interference ability

Inactive Publication Date: 2016-06-15
SHENZHEN LEADMED MEDICAL TECH
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of low sensitivity, unstable signal and poor anti-interference ability of the existing rapid diagnostic test paper, the present invention provides a method that uses far-infrared nano-fluorescent particles to realize color reaction, and has stable signal, strong anti-interference ability, and is not immune to interference. Rapid diagnostic test paper with external light interference and high sensitivity, and preparation method of the rapid diagnostic test paper

Method used

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  • A kind of rapid diagnostic test paper and preparation method thereof
  • A kind of rapid diagnostic test paper and preparation method thereof
  • A kind of rapid diagnostic test paper and preparation method thereof

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preparation example Construction

[0024] The preparation method of quick diagnostic test paper of the present invention is:

[0025] (1) At room temperature, the fluorescent particle solution is mixed with the SMCC solution, and the mass ratio of the fluorescent particle to the SMCC in the solution is 0.1-0.15:48-96, and left standing for 0.5-1.5h to obtain a mixed solution; then remove the mixed solution salt (according to the prior art, desalting through a desalting column), and adding a phosphate buffer solution with a pH of 7.0-7.4 to the mixed solution until the mass volume concentration of the fluorescent particles in the mixed solution is 100-300ng / mL to obtain a preparation solution.

[0026] Take 10-20 nitrotyrosine antibody solutions with a volume concentration of 200-400ng / mL, mix it with 1 volume of the preparation solution and let it stand for 20-50min, then remove the salt in the preparation solution (according to the prior art, Desalting through a desalting column) to obtain a preparatory soluti...

Embodiment 1

[0033] refer to Figure 1-3 , a rapid diagnostic test paper for diagnosing kidney disease provided in this embodiment, comprising a bottom plate 6, a nitrosated sample pad 1, a nitrosed binding pad 2, a nitrosed reaction membrane 3, a nitrosed absorbent pad 4 and Plastic support frame 5. A sampling window 51 and an observation window 52 are provided on the plastic support frame 5 , and the bottom plate 6 is packaged in the plastic support frame 5 . The nitrosation sample pad 1 , the nitrosation binding pad 2 , the nitrosation reaction membrane 3 and the nitrosation absorption pad 4 are sequentially arranged on the bottom plate 6 and closely connected. Simultaneously, a detection zone 32 and a quality control zone 31 are arranged in parallel on the nitration reaction membrane 3, and the detection zone 32 is positioned between the quality control zone 31 and the nitration binding pad 2, such as figure 1 and figure 2 shown. The detection area 32 is the area coated with the a...

Embodiment 2

[0050] This embodiment provides a rapid diagnostic test strip for diagnosing heart disease, the structure of which is the same as that of the rapid diagnostic test strip for diagnosing kidney disease in Embodiment 1.

[0051] The preparation steps of the rapid diagnostic test paper for diagnosing heart disease are as follows:

[0052] (1) Fluorescent particle solution: the particle size is 40nm, the fluorescent wavelength is 700nm, the solvent is dithiothreitol, the concentration of fluorescent particles is 800nmol / L, and the mass volume concentration is 1mg / L.

[0053] At room temperature, take 150 μL of fluorescent particle solution, add 15 μL of 4.8 mg / mL SMCC solution to it, mix well and let stand for 1 hour to obtain a mixed solution. Then put the mixed solution into a desalting column for desalting treatment, and collect the desalted mixed solution in a centrifuge tube by low-speed centrifugation (1000-5000rmp). Then, the mixed solution was diluted to 500 μL with a phos...

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Abstract

Disclosed are a rapid diagnosis strip and the preparation method thereof. The rapid diagnosis strip contains a binding pad coated with a nitrotyrosine antibody-fluorescent particle marker, a sample pad, a reaction film and an absorption pad, wherein a nitrotyrosine antibody-fluorescent particle marker spraying liquid is prepared before performing the processes of spraying, drying and sealing. Due to the character of far infrared fluorescent particles, there is no quenching phenomenon appearing for the tracer used in the invention, and the diagnosing strip doesn't have its own background far infrared fluorescence. This can significantly improve the signal stability and the anti-interference capability of the rapid diagnosis strip. Cooperating with the nitrated sample pad, the binding pad, the reaction film and the absorption pad, the specificity, sensitivity and veracity of the diagnosing strip can be further improved. When the fluorescent particle solution is mixed with a SMCC solution in advance, activating the activity of the fluorescent particles and then making them bind with the nitrotyrosine antibody, the stability of the tracer can be improved, thereby facilitating the improvement of the specificity, sensitivity and veracity of the diagnosing strip.

Description

technical field [0001] The invention relates to the technical field of in vitro detection, in particular to a rapid diagnostic test paper and a preparation method thereof. Background technique [0002] At present, the incidence of various diseases such as cardiovascular disease, liver disease, kidney disease, diabetes and tumors is increasing year by year. People have realized that the rapid diagnosis and early detection of diseases has become more and more important. Scientists have made many attempts and breakthroughs for this purpose, such as gene chip technology, radioimmunoassay, enzyme-linked immunosorbent technology, etc., but because these methods require professional operation, and the operation is complicated, the steps are cumbersome, and some even involve radioactive substances. , Therefore, it is difficult to effectively diagnose the disease early and quickly. However, rapid diagnostic test strips are a popular and important method for rapid and early diagnosis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543G01N33/533
CPCG01N33/52G01N33/68
Inventor 岳朋周锦源盛威玮
Owner SHENZHEN LEADMED MEDICAL TECH
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