Label-free light reflection interference optical fiber biosensor

Abstract

The invention discloses a label-free light reflection interference optical fiber biosensor which belongs to the technical field of biochemical equipment. The label-free light reflection interference optical fiber biosensor is characterized in that a full-wave band halogen tungsten lamp is used as a light source, and broadband light is coupled in an optical fiber through a lens collimator; a Y-shaped optical fiber coupler, a source optical fiber connected with a light source, a transmission optical fiber connected with an optical fiber probe and a detection optical fiber connected with a rear beam splitting system are adopted; the optical fiber probe is composed of a multi-mode optical fiber, and the far end of the optical fiber probe is provided with three layers, namely a high-refraction index interference layer, a chemical reagent modified layer and a biomolecule layer, and incident light is subjected to two reflection on the high-refraction index interface layer and the biomolecule layer at the end face; the rear beam splitting system comprises an incident slit, a concave mirror, an optical grating and an array receiver, and the detection on a to-be-tested signal is realized through a signal acquirer. The label-free light reflection interference optical fiber biosensor has the beneficial effects that a sample does not need to be marked and purified based on a light interference principle and a principle of interaction among biomolecule, and the stability and the accuracy of detection are ensured; no other reagents are required to be added during the detection; a dynamic process can be directly detected in real time and in place, and the detection on existence of a to-be-detected material can be simultaneously realized; the label-free light reflection interference optical fiber biosensor is suitable for measurement of a sample on the spot; the man-machine interaction is realized, and the operation is simple.

Description

technical field [0001] The invention belongs to the technical field of biochemical equipment, and relates to a detection instrument for samples to be tested in the fields of biology, medicine, agriculture, food and the like. Background technique [0002] Optical fiber biosensors use optical fiber as a conducting medium and collect signals for detection. They can adapt to extremely harsh environments, are free from electromagnetic interference, corrosion resistance, safe signal transmission, low loss, non-destructive and easy to use, and have attracted widespread attention. Among the optical fiber biosensors, the most widely used is the optical fiber evanescent wave biosensor, which utilizes the evanescent wave generated at the optical fiber probe when the light wave is transmitted in the optical fiber in the form of total reflection, and the evanescent wave excites the surface of the sensor probe. The connected biological substances labeled with fluorescent molecules generat...

AI Technical Summary

Problems solved by technology

However, the energy of the evanescent wave field is small, and the excited fluorescent signal is weak; the sample to be detected needs fluorescent labeling, but the stability of the fluorescent label is poor; The stability of the large interference

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