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A technology of biosensors and optical fibers, applied in biological testing, fluorescence/phosphorescence, instruments, etc., can solve problems such as patent achievement reporting, and achieve the effects of good selectivity, high sensitivity, and portable size
Inactive Publication Date: 2009-12-23
DALIAN MARITIME UNIVERSITY
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At present, the research and development of sensing equipment matching the above-mentioned fluorescent labels is in its infancy, and no corresponding patent results have been reported.
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[0029] The Y-shaped optical fiber bundle 9 used in the optical fiber fluorescent biosensor is formed by bundling seven multi-film optical fibers, one in the center and six in total around. Such as figure 2 As shown, at one end of the Y-shaped optical fiber bundle 9, the central optical fiber separates from the optical fiber bundle 9 to form a branch of the Y-shaped optical fiber bundle 9, that is, a branch 10; other peripheral optical fibers are bundled to form another branch of the Y-shaped optical fiber bundle 9 , namely the branch 11;
[0030] The branch 10 of Y-shaped fiber bundle 9 is made up of a single multi-film optical fiber 13 in the center of Y-shaped fiber bundle 9, and its cross-sectional schematic diagram is as follows image 3 shown. The inner layer of the central multi-film optical fiber 13 is the core 14, and its material is high-purity silicon oxide. The outer layer of the fiber core 14 is cladding material 15, which is high-purity silicon oxide. On the ...
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Abstract
The invention relates to an optical fiber fluorescent biological sensor, which is composed of an excitation light path, a detection light path and a fluorescence receiving light path, and the three light paths are respectively connected to three branches of a Y-shaped optical fiber. On the excitation optical path, the excitation light source is coupled to one branch of the Y-shaped optical fiber by coupling optics; on the detection optical path, the other branch of the Y-shaped optical fiber is connected to the carrier or sample pool for immobilizing the biological recognition agent and performing sample detection; On the fluorescence receiving optical path, the third branch of the Y-shaped optical fiber is connected to the data acquisition system, and further connected to the signal processing and display system. The sensor has fast response, high sensitivity, good selectivity, light weight, small size, and is biologically friendly. It is suitable for all current fluorescent markers and can overcome the outstanding defects of traditional fluorescent markers such as short luminescence lifetime and photobleaching. It is widely used in Biomolecular recognition, medical clinical diagnosis, high-throughput drug screening, environmental monitoring, biochemical warfare agent detection and many other fields.
Description
technical field [0001] The invention relates to an optical fiber fluorescent biosensor, which can be widely used in many fields such as medical diagnosis, biomolecules, drug screening, environmental monitoring, food sanitation detection, biochemical warfare agent detection and the like. Background technique [0002] A sensor is a component that can sense a certain signal to be measured and convert it into sound, light, electricity and other signals, including sensitive components, conversion components and corresponding circuits. There are many types of sensors, among which the sensors composed of biological materials such as antigens, antibodies, enzymes, nucleic acids, cells, etc. , FOBS), this sensor determines the amount of the substance to be tested by detecting the light generated by the biological reaction, and by detecting the wavelength, intensity, amplitude, phase and other parameters of the light. Compared with other sensors, this sensor has the advantages of str...
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