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Complement C3 detection kit and preparation thereof

A detection kit and kit technology, applied in the biological field, can solve the problems of difficult observation, large variation, inaccurate diameter measurement, etc.

Inactive Publication Date: 2015-08-19
王贤俊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The one-way immunodiffusion method is time-consuming and highly variable, the results are not easy to observe, and the measurement diameter is inaccurate; the ELISA method has poor repeatability, is prone to false positives, and is most affected by temperature and time; the immunoturbidimetric method is relatively better than the previous two methods , but has the disadvantage of requiring a large amount of antiserum

Method used

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  • Complement C3 detection kit and preparation thereof
  • Complement C3 detection kit and preparation thereof
  • Complement C3 detection kit and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) Determination of precision: 20 consecutive samples were taken for measurement, and the mean, standard deviation and coefficient of variation of the measured values ​​were calculated. CV = SD X ‾ × 100 %

[0030] Table 1 Precision test results

[0031]

[0032]

[0033] The coefficient of variation CV is usually used to measure the precision of a determination method, the smaller the CV value, the better the precision of the result of the determination method. For clinical chemistry tests, a method precision with a CV of less than 5% is generally accepted as acceptable. The CV value in Table 1 is less than 3%, indicating that the method of the present invention has excellent precision.

[0034] 2) Measurement of accuracy: use the same quality control product, repeat the measurement 3 times, take the average value, and the relative deviation...

Embodiment 2

[0045] Embodiment 2 The linear correlation determination of reagent of the present invention and commercially available reagent A:

[0046] Respectively adopt reagent of the present invention and commercially available reagent A, adopt Olympus 400 full-automatic biochemical analyzers, to 50 samples (comprising normal and abnormal sample), measure by respective parameter, and carry out correlation analysis to measured value (result See figure 2 , the X-axis represents the measured value of the test of the present invention, and the Y-axis represents the measured value of the commercially available reagent A). Correlation coefficient: r 2 =0.9948, the linear equation is: y=1.041x-0.054, the results show that this reagent has a good correlation with commercially available reagents.

[0047] Table 4 Linear correlation test results

[0048]

[0049]

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Abstract

The invention provides a complement C3 detection kit, which is a liquid double reagent, including a reagent R1 and a reagent R2; the reagent R1 contains pH 6.0 phosphate buffered saline, Tween 20, Ethylene Diamine Tetraacetic Acid (EDTA-2Na), sodium chloride, polyethylene glycol (PEG 6000) and sodium azide; the reagent R2 is a solution containing complement C3 latex particles. The complement C3 detection kit is characterized in that by crosslinking by using a chemical process, water-soluble carbodiimide (EDC) and N-hydroxysuccinimide (NHS) are utilized to enable goat anti-human complement C3 antiserum and carboxylated polystyrene latex to be subjected to covalent cross linking, thus forming the complement C3 latex reagent. The reagent is high in sensitivity, high in specificity, simple in reagent preparation, and worth of being popularized and used further.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and provides a complement C3 detection kit and its preparation, which have the characteristics of high sensitivity, strong specificity and simple reagent preparation, and are worthy of further popularization and use. Background technique: [0002] C3 is the complement component with the highest content in serum. It is a kind of β globulin synthesized by macrophages, monocytes, lymphoid tissue, bone marrow, peritoneum and liver. Under the action of C3 convertase, it is cracked into C3a and C3b. It plays an important role in both the classical and alternative pathways of complement activation. [0003] C3 is the one with the highest content among the components of complement, and is the most important link in the complement activation pathway, so the determination of its content is very important. The increase and decrease of C3 are basically consistent with the total complement activity, but more se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N33/68
Inventor 王贤俊郑蓓蕾郭二豪江新涛
Owner 王贤俊
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