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Gastric cancer detecting method, reagent and gastric cancer detecting kit

A detection method and kit technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of short validity period, unpopularization, slow detection speed, etc., achieve good diagnosis and screening, improve early diagnosis, and low detection cost Effect

Inactive Publication Date: 2015-05-27
PUREBIO LAB NINGBO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although RIA has high sensitivity, the reagents are radioactive, causing environmental pollution, and the validity period is short, difficult to store, and the application is limited; TRFIA has the highest sensitivity, but the cost of reagents is also high, and expensive instruments are required; the sensitivity of ELISA Relatively low, and can only be used for qualitative or semi-quantitative detection, and cannot dynamically observe the changes of PGⅠ. The operation steps of these three methods are cumbersome manual operations, and the detection speed is relatively slow
As a new tumor marker, PG is of great significance for the early diagnosis and screening of gastric cancer, the detection of recurrence and metastasis after gastric cancer surgery, the differentiation of benign and malignant ulcers, and the evaluation of gastric acid secretion. The method is not yet popular in our country

Method used

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  • Gastric cancer detecting method, reagent and gastric cancer detecting kit
  • Gastric cancer detecting method, reagent and gastric cancer detecting kit
  • Gastric cancer detecting method, reagent and gastric cancer detecting kit

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Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0065] 1. The main components and concentration of the kit:

[0066] Reagent R1 includes: glycine buffer solution, pH 8.0, with a concentration of 50mmol / L; sodium azide, with a mass percentage of 0.09%; bovine serum albumin, with a mass percentage of 0.60%; sodium chloride, with a mass percentage of 0.90%; PEG6000 , the mass percentage is 3.00%; Tween20, the mass percentage is 0.10%.

[0067] Reagent R2 includes: Tris buffer solution, pH8.0, concentration is 50mmol / L; sodium azide, mass percentage is 0.09%; sodium chloride, mass percentage is 0.90%; Tween20, mass percentage is 0.10%; bovine serum albumin , the mass percentage is 0.60%; ethylene glycol, the mass percentage is 8.00%; latex particles, the percentage content is 0.40%, coated with PGⅠantibody, and the particle size is 100nm.

[0068] Reagent R3 includes: Tris buffer, PH8.0, concentration is 50mmol / L; Sodium azide, mass percentage is 0.09%; Sodium chloride, mass percentage is 0.90%; Tween20, mass percentage is 0.1...

specific Embodiment 2

[0072] 1. The main components and concentration of the kit:

[0073] Reagent R1 includes: Tris buffer, pH8.0, concentration is 50mmol / L; Glycine, mass percentage is 0.40%; Sodium azide, mass percentage is 0.09%; Bovine serum albumin, mass percentage is 0.60%; , the mass percentage is 0.90%; PEG6000, the mass percentage is 4.00%; Emulgen B-66, the mass percentage is 0.20%.

[0074] Reagent R2 includes: Tris buffer solution, pH8.0, concentration is 50mmol / L; sodium azide, mass percentage is 0.09%; sodium chloride, mass percentage is 0.90%; Tween20, mass percentage is 0.10%; bovine serum albumin , the mass percentage is 0.60%; sucrose, the mass percentage is 10.00%; latex particles, the percentage content is 0.35%, coated with PGⅠantibody, and the particle size is 150nm.

[0075]Reagent R3 includes: Tris buffer, PH8.0, concentration is 50mmol / L; Sodium azide, mass percentage is 0.09%; Sodium chloride, mass percentage is 0.90%; Tween20, mass percentage is 0.10%; Bovine serum albu...

specific Embodiment 3

[0079] 1. The main components and concentration of the kit:

[0080] Reagent R1 includes: Tris buffer solution, PH7.0, concentration is 20mmol / L; Potassium sorbate, mass percentage is 0.05%; Bovine serum albumin, mass percentage is 1.80%; Magnesium chloride, mass percentage is 0.90%; The percentage is 4.00%; Tween80, the mass percentage is 0.90%; EDTA, the mass percentage is 0.50%; lactose, the mass percentage is 0.01%.

[0081] Reagent R2 comprises: glycine buffer solution, pH7.0, concentration is 80mmol / L; Sodium azide, mass percentage is 0.10%; Potassium sorbate, mass percentage is 0.05%; Sodium chloride, mass percentage is 0.20%; Tween20, The mass percentage is 0.10%; SDS, the mass percentage is 1.00%; bovine serum albumin, the mass percentage is 0.60%; ethylene glycol, the mass percentage is 8.00%; latex particles, the mass percentage is 0.20%, coated with PGⅠ antibody , the particle size is 200nm.

[0082] Reagent R3 comprises: glycine buffer solution, pH7.0, concentra...

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Abstract

The invention provides a gastric cancer detecting method, a reagent and a gastric cancer detecting kit. The kit comprises a reagent R1 and a reagent R2 or R3; in vitro, the serum of a tested body is mixed with the reagent R1, and the reagent R2 or R3 is added after several minutes; latex particles included in the reagent R2 or R3 are coated with PG antibodies and can be combined with the PG antigen in a sample to form an insoluble complex; based on a latex-enhanced immunoturbidimetry principle, the absorbancy tested by a biochemical analyzer is compared with that of a calibration product, so that the content level of PG is calculated; compared with the content level of the serum PG of healthy people, the content level of PG shows that a health condition of the stomach of the tested human body is initially judged. By comparing a test result obtained by using the kit with that obtained by using an imported kit A, the kit has the advantages that the extremely-high correlation is achieved, and the correlation coefficient is more than or equal to 0.9750. The kit provided by the invention can be used for detecting the gastric cancer, and has stable and reliable detection results; moreover, the use cost can be reduced.

Description

technical field [0001] The invention relates to a gastric cancer detection method, which can quickly and accurately detect gastric cancer quantitatively through the reaction between pepsinogen antigen and antibody and adopts the principle of latex-enhanced immune turbidimetry, and relates to a method capable of combining pepsin antigen and antibody A combined kit for detecting gastric cancer and related reagents. Background technique [0002] The stomach is the organ that holds food in the abdominal cavity. It is mainly used to grind large pieces of food into small pieces and degrade the large molecules in the food into smaller molecules for easy absorption. For humans, the stomach is a very important one digestive organs. [0003] In modern society, due to the unhealthy lifestyle of human beings, the damage to the stomach is great, coupled with insufficient protection, the incidence of gastric cancer is extremely high, which seriously affects people's quality of life. In m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57446G01N33/54393G01N33/544
Inventor 许国和胡露群范翠翠
Owner PUREBIO LAB NINGBO
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