57 results about "Buffered phosphate" patented technology

The present invention relates to a kind of silk fibroin hydrogel and preparation method thereof, silkworm raw silk is degummed, sol, dialyzed, filtered, freeze-dried to obtain silk fibroin freeze-dried powder, then redissolved into high concentration silk fibroin solution, filtered and spray-dried into silk fibroin microspheres; silk fibroin microspheres are slowly added to a phosphate buffered saline solution containing γ-polyglutamic acid and glutaraldehyde, stirred and ultrasonically carried out the first cross-linking, then a cross-linking agent solution is added, stirred evenly and ultrasonically, and the second cross-linking is carried out, and the silk fibroin hydrogel is obtained after being rinsed with pure water after being placed for a period of time. In the preparation method of the present invention, a phosphate buffered saline solution containing γ-polyglutamic acid and glutaraldehyde is used as a cross-linking agent and secondary cross-linking is carried out, and the prepared silk fibroin hydrogel silk fibroin has a long retention time, good stability and mechanical strength, and the cross-linking method is simple, and it is suitable for large-scale industrial production.

A method for preparing exosomes is provided. The method includes preparing a human platelet, treating the human platelet with a buffer to obtain a platelet solution, centrifuging the platelet solution, and collecting supernatant of the centrifuged platelet solution to obtain an exosome solution. The buffer is calcium ion buffer, phosphate buffered saline (PBS), tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) buffer, or 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES) buffer.

The invention discloses a preparation method of a drug-loaded glycoprotein-like network hybridization tissue engineering valve material, which comprises the following steps: carrying out a one-pot hybridization cross-linking reaction on a decellularized biological material, oxidized hyaluronic acid, phenylboronic acid grafted silk fibroin and curcumin to construct and form a drug-loaded glycoprotein-like network on the biological material; the biological material is animal tissue, including but not limited to any one of pericardium, valve, intestinal membrane, meninx and the like. The method comprises the following steps: soaking a decellularized biological material in a solution A containing OHA, SF-PBA and curcumin, and continuously oscillating for 48 hours to carry out a cross-linking reaction; and then washing with phosphate buffer saline water and distilled water to obtain the drug-loaded glycoprotein-like network hybrid tissue engineering valve material. The prepared tissue engineering valve material overcomes the defects that traditional decellularized valve and glutaraldehyde cross-linked valve materials are easy to calcify, have toxicity and are poor in immunogenicity.

The invention discloses a method for detecting phenyl sulfide in rectification residual liquid of 2, 4-dichlorophenol through high performance liquid chromatography, and belongs to the technical field of chemical detection and analysis. The method comprises the following steps: dissolving a to-be-detected 2, 4-dichlorophenol rectification residual liquid sample to prepare a to-be-detected sample, and preparing phenyl sulfide standard substance solutions with different concentrations; the method comprises the following steps: determining a to-be-detected sample and a phenyl sulfide standard substance solution by adopting a high performance liquid chromatograph, drawing a standard curve and a linear regression equation according to a detection result of the phenyl sulfide standard substance solution, and analyzing the phenyl sulfide in the sample by combining the linear regression equation and a chromatographic peak map of the to-be-detected sample, wherein the mobile phase comprises a phase A and a phase B, the phase A is an aqueous solution of phosphate buffer salt, the phase B is methanol, and an isocratic elution mode is adopted. According to the present invention, with the method, the accurate determination of the phenyl sulfide content in the 2, 4-dichlorophenol rectification residual liquid is achieved, the timely and accurate data guidance is provided for the 2, 4-D production process, and the target of production cost saving is ultimately achieved.

The invention belongs to the technical field of resveratrol synthesis, and particularly relates to a preparation method of resveratrol and application of resveratrol in meat preservation. The method comprises the following steps: taking 3, 5-dimethoxybenzoic acid as a raw material, and carrying out acylation reaction to obtain 3, 5-dimethoxybenzoyl chloride. Then reacting with p-acetoxystyrene to generate 3, 3 '-dihydroxy-3, 3'-dihydroxy-3 The preparation method comprises the following steps: taking 1, 5-dimethoxy-4 '-acetoxystilbene as a raw material, hydrolyzing and demethylating to obtain resveratrol, and preparing resveratrol lipid nanoparticle suspension by taking resveratrol, a ZIF-8 / chitosan compound, compound lipid, cholesterol, epsilon-polylysine, ascorbyl palmitate and phosphate buffer saline as raw materials. High load and controlled release of resveratrol are achieved, and the problems that resveratrol is poor in water solubility and prone to oxidation are solved.

The invention belongs to the technical field of hydrogel preparation, and particularly relates to sodium alginate-based hydrogel and a preparation method thereof. The raw materials of the kit comprise the following components in parts by weight: 20 mL of morpholinoethanesulfonic acid (MES) buffer solution, 0.06 g of N-hydroxysuccinimide (NHS), 0.2 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), 0.2 g of RGD polypeptide, 0.1 g of a pH regulator, 0.1 g of a pH regulator and the balance of deionized water. The hydrogel comprises 175 [mu] L of an EDC / NHS activation solution, 1 g of sodium alginate (SA), 51 mL of a phosphate buffer solution (PBS), 1.44 g of low-acylated gellan gum powder, 0.16 g of high-acylated gellan gum powder, 0.1 g of RGD-SA powder, 0.2 g of VN-SA powder, 0.1 g of QK-SA powder, a VN polypeptide according to a molar ratio of VN to SA being 1: 15, and a QK polypeptide according to a molar ratio of QK to SA being 1: 15. And the biofilm has the characteristics of controllable mechanical strength and slow degradation, and is beneficial to long-term culture and tissue repair of cells.

The invention relates to a method for detecting related substances of an iluaceae intermediate I, and belongs to the technical field of pharmaceutical analysis. According to the method, octadecylsilane chemically bonded silica is adopted as a chromatographic column of a filling agent, a phosphate buffer solution-methanol-acetonitrile is adopted as a mobile phase, a gradient elution mode is adopted, and the established high performance liquid chromatography method can effectively measure the content of the intermediate I, namely the content of 2-(dimethyl phosphoryl)-N-(2, 5-dichloropyrimidine) aniline and related impurities. The method is good in specificity, high in sensitivity and convenient to operate, and provides an effective guarantee for quality control of an iluaceae final product. The invention also provides a novel impurity reference substance compound and a preparation method thereof.

The present disclosure proposes a preparation method of a vesicle formed by an erythrocyte membrane encapsulating Newcastle disease virus, including: mixing a compacted erythrocyte solution, a phosphate buffered saline (PBS) solution, and Newcastle disease virus (NDV); centrifuging a resulting mixture of erythrocytes added with NDV; and resuspending and precipitating the centrifuged mixture with a PBS solution to obtain the vesicle formed by the erythrocyte membrane encapsulating Newcastle disease virus.

The invention relates to the technical field of biomedical sample preservation, and discloses a preserving fluid of porcine fat-derived mesenchymal stem cell exosomes, a preparation method and application thereof. The exosome preserving fluid provided by the invention comprises the following components in percentage by weight: 5%-20% of polyvinylpyrrolidone (PVP); 2%-10% of trehalose; 0.5%-5% of glycerol; according to the cryoprotectant, a cryoprotectant is adopted, a phosphate buffer solution (PBS) is adopted as a solvent, polyvinylpyrrolidone (PVP) is adopted to replace traditional dimethyl sulfoxide (DMSO) to serve as the cryoprotectant, the problems that in the prior art, a preserving fluid is large in toxicity and poor in preserving effect are solved, and the application of the cryoprotectant in medical external application such as wound repair, inflammation treatment and skin care is expanded.

A radioactive yttrium phosphate suspension, including a phosphate buffered saline solution and a plurality of radioactive yttrium phosphate particles suspended in the phosphate buffered saline solution. The plurality of radioactive yttrium phosphate particles are between 0.1 um to 2 um in diameter. The plurality of radioactive yttrium phosphate particles are generally spherical. The YPO4 particle concentration is in the range of 40 mg / ml to 125 mg / ml.

The present invention relates to the technical field of detection and analysis, and in particular to a method for determining the chromatographic purity of 2,4,6-trimethylbenzenesulfonyl chloride by utilizing HPLC. The method comprises the following conditions: a stationary phase is an octadecylsilane bonded phase, a mobile phase comprises a mobile phase A and a mobile phase B, the mobile phase A is a potassium dihydrogen phosphate buffered saline solution, and the mobile phase B is acetonitrile; the method can effectively separate 2,4,6-trimethylbenzenesulfonyl chloride, mesitylene, and a hydrolysis impurity 2,4,6-trimethylbenzenesulfonic acid in a 2,4,6-trimethylbenzenesulfonyl chloride product, has high separation degree and high sensitivity, does not generate interfering impurities, and can accurately determine the purity of the 2,4,6-trimethylbenzenesulfonyl chloride in the product.

Disclosed here includes a method for purifying a biologic composition, comprising diafiltering the biologic composition into a composition comprising phosphate buffered saline (PBS) to obtain a purified composition. The method disclosed here can be particularly useful for removing one or more impurities from the biologic composition, such as bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane (Bis-tris).

A method of preparing a FeMnCeOx biomaterial is provided, including the following steps. A Pseudomonas sp. strain KW-2 is obtained. A culture medium with a pH of 6.5-7.8 is prepared, which includes 0.1 g / L K2HPO4, 0.2 g / L MnSO4·7H2O, 0.2 g / L NaNO3, 0.1 g / L CaCl2), 0.1 g / L NH4Cl, 0.1 g / L (NH4)2CO3, 35 g / L NaCl and 150 mg / L ferric ammonium citrate. The culture medium is autoclaved, inoculated with the KW-2 strain, cultured for 1-3 days, added with a cerium nitrate solution, cultured for 3-7 days and centrifuged at 4,000-8,000 rpm for 10-20 min to collect a precipitate. The precipitate is rinsed 5-8 times with deionized water and 0.01 mol / L phosphate buffered saline (PBS) and freeze-dried at −60° C. to obtain the FeMnCeOx biomaterial. A method for treating antibiotic wastewater using the FeMnCeOx biomaterial is also provided.

The present disclosure relates to a process to assemble proteins derived from platelet lysates in bioactive microparticles, with increased surface organization. The present invention further relates to protein-based biomaterials applicable to biomedical and biotechnology fields, more precisely in tissue engineering strategies, disease modeling, and other biomedical applications. Namely, a method for obtaining a protein microparticle from a platelet lysate comprising the following steps: lyophilizing the platelet lysate; dissolving the lyophilized platelet lysate in phosphate buffer saline to obtain a platelet lysate solution; adding a reducing agent to the platelet lysate solution to obtain a reduced platelet lysate solution; adding an oxidizing agent to the reduced platelet lysate solution to form the microparticles by precipitation.

The present application discloses a 3D printing method, comprising the following steps: weighing a phosphate buffered saline solution or / and deionized water and placing them in a mixing container, then placing the container in a refrigeration device and cooling it to 4°C, weighing a preset mass of poloxamer and adding it to the mixing container, and maintaining the temperature of the refrigeration device at 4°C until the poloxamer is completely dissolved; heating the solution after the poloxamer is completely dissolved to 50°C, gradually adding a preset mass of hydrophobic treated hydroxypropyl methylcellulose and stirring for 20 minutes to completely dissolve it, and obtaining a support bath material; adding an additive to the support bath material; using a 3D printing material to perform 3D printing in a mixture of the support bath material and the additive; after the 3D printing is completed, using an eluent to at least elute the support bath material. The present application is beneficial in that it provides a 3D printing method for printing using a support bath material with good stability, biocompatibility and rheological properties.

The present application provides a visible light crosslinking composite gel type hemostatic dressing, comprising 1-55% methacrylated natural polymer, 0.0001-0.05% visible light initiator, 2-15% thickening agent; the method for preparing the hemostatic dressing comprises the following steps: firstly, preparing a visible light initiator solution, and dissolving the methacrylated natural polymer with the visible light initiator solution to obtain solution A; dissolving the thickening agent with deionized water or a phosphate buffered saline solution to obtain solution B; uniformly mixing solution A and solution B to obtain the visible light crosslinking composite gel type hemostatic dressing. The present application (1) is suitable for first aid hemostasis; (2) is solidified under visible light irradiation and closely adheres to the wound tissue; (3) has good adhesion, flexibility and moisturizing property after being solidified under visible light irradiation, and has a certain healing promotion effect.

The invention discloses a preparation method of a soybean seed coat polysaccharide encapsulated essential oil liposome antibacterial agent, which comprises the following steps: uniformly dispersing soybean lecithin (20mg / ml), stigmasterol (4mg / ml) and essential oil (1%) in absolute ethyl alcohol, carrying out rotary evaporation at 45 DEG C until a uniform lipid film is formed in a bottle, transferring into a vacuum drying oven, and further drying and removing ethyl alcohol; the method comprises the following steps: carrying out ultrasonic treatment on a liposome sample, hydrating by using a phosphate buffer solution (PBS, 0.01 mol / l, pH of 7.2-7.4) containing Tween-80, and carrying out ultrasonic treatment for 10 minutes under the power of 200W to obtain the liposome sample; uniformly mixing the soybean seed coat polysaccharide solution (0.01%-0.2%) with the liposome solution according to the volume ratio of 1: 1, and stirring for 1 hour to obtain a soybean seed coat polysaccharide encapsulated liposome solution; the slow-release antibacterial agent prepared by the preparation method disclosed by the invention has excellent slow-release effect and antibacterial property, and the preparation method is simple to operate and low in cost.

The application relates to the technical field of medical biomaterials, in particular to a nanofiber composite hydrogel gastrointestinal patch and a preparation method thereof, which comprises the following steps: dissolving polycaprolactone in trifluoroethanol to obtain solution A; dispersing T-5224 in the solution A, uniformly mixing and then performing electrospinning to obtain a nanofiber membrane; adding carboxymethyl chitosan into a phosphate buffered saline solution, stirring until completely dissolved to obtain an A-component solution; sequentially adding sodium alginate oxide and tannic acid into the phosphate buffered saline solution, stirring until completely dissolved to obtain a B-component solution; mixing the A-component solution and the B-component solution in proportion, uniformly stirring, then smearing the mixture on the surface of the nanofiber membrane, and standing until the hydrogel is formed to obtain the nanofiber composite hydrogel gastrointestinal patch. In the application, the nanofiber membrane is used as a substrate, the hydrogel coating layer is crosslinked to form a stable three-dimensional network structure, and the mechanical property of the overall patch can meet the support requirement in the gastrointestinal repair process.

Disclosed herein are polymeric nanoparticles containing peptides, which provide low burst release and sustained, delivery of the peptides, and pharmaceutical compositions thereof. The polymeric nanoparticles contain a peptide encapsulated or dispersed therein. The nanoparticles can provide sustained release of the peptide, for example, less than 20% of the peptide is released initially (at time 0 hour) following placement into a phosphate buffered saline at pH 7.4 at 37° C. and room pressure. Methods for micronizing a peptide and for preparing polymeric nanoparticles containing solid, micronized peptides are also disclosed. The preparation methods use miscible solvent and non-solvent pairs in phase inversion nanoencapsulation processes. The Gibbs energy of mixing (ΔGMix) between the solvent and non-solvent can be tailored to achieve desired particle size, encapsulation efficiency, and release profile.

Disclosed herein are compositions for fixing tissue for cytologic, histomorphologic, and / or molecular analysis (e.g., DNA, RNA, and / or protein analysis). In some embodiments, the fixatives are aldehyde-free fixatives, for example, formaldehyde- or formalin-free fixatives. Particular disclosed compositions include buffered ethanol. The buffer is a phosphate buffer or phosphate buffered saline (PBS) in some examples. In further embodiments, the fixative includes additional components, such as glycerol and / or acetic acid.

The invention belongs to the technical field of fine chemicals, and provides a bionic nano melanin protective agent with photo-thermal responsiveness and an ultraviolet-visible light dual-band protection function as well as a preparation method and application of the bionic nano melanin protective agent. The preparation method comprises the following steps: mixing dopamine hydrochloride with a Tris buffer solution for reaction, and then carrying out light-shielding reaction with a tris (hydroxymethyl) aminomethane hydrochloride solution and sulfydryl polyethylene glycol to obtain PDA-coated PEG nanoparticles; the preparation method comprises the following steps: mixing Pluronic F-127 with phosphate buffered normal saline, and reacting to obtain PF-127 hydrogel; and mixing the PF-127 hydrogel with the PDA-coated PEG nanoparticles, and carrying out a light-shielding reaction, so as to obtain the bionic nano melanin protective agent with photothermal responsiveness and an ultraviolet-visible light dual-band protection function. The method is simple, green and environment-friendly, and the obtained protective agent has photo-thermal response performance and a lasting and safe sunscreen effect and has good application potential in the aspect of cold skin disease protection.

The invention belongs to the technical field of medical materials, and particularly relates to chondroitin sulfate composite repairing hydrogel as well as a preparation method and application thereof. The hydrogel is prepared from the following raw materials in parts by weight: 5 to 15 parts of gelatin, 1 to 5 parts of chondroitin sulfate, 0.5 to 2 parts of glutaraldehyde, 2 to 8 parts of glycerol and 70 to 92 parts of phosphate buffer saline. The specific preparation method comprises the following steps: adding a chondroitin sulfate solution into a gelatin solution, mixing, adding glycerol, mixing again, adding glutaraldehyde diluted by phosphate buffer saline water while stirring, degassing after stirring to obtain a hydrogel solution, pouring the hydrogel solution into a mold, standing for forming, soaking in the phosphate buffer saline water, and drying to obtain the chondroitin sulfate hydrogel. And finally, sterilizing to obtain the chondroitin sulfate composite repairing hydrogel. Through the unique component proportion and the preparation process, the contradiction between the mechanical property and the biological activity of the traditional material is solved, and the synergistic enhancement of multiple functions is also realized.

The invention discloses a treatment method for strengthening active components of medicinal and edible medicinal materials, which comprises the following steps: adding a phosphoric acid buffer salt solution and cellulose compound enzyme into medicine residue thick slurry, carrying out enzymolysis reaction for 2-8 hours in an acid environment of 45-55 DEG C by adopting ultrasonic oscillation, and collecting enzymatic hydrolysate; or performing particle crushing and homogenizing treatment by using the pressure of 30-60MPa, performing 2-10 times of volume circulation treatment capacity, performing oscillation treatment on the cell crushing liquid by using 30-80W ultrasonic waves, and collecting filtrate; performing differential centrifugation on the enzymatic hydrolysate or / and the filtrate to obtain an exosome solution; and adding the exosome solution into the concentrated original liquid medicine, and stirring and heating at 80-90 DEG C for 30-60 minutes. Residual cell exosomes are extracted from medicine residues of medicinal and edible medicinal materials and added into original Chinese herbal medicine liquid, Maillard reaction is carried out through heat treatment, and the oxidation resistance of the raw medicinal materials can be improved by 15% or above.

This invention discloses a tannic acid-reinforced anisotropic polyvinyl alcohol hydrogel, its preparation method, and its applications. The preparation method of the hydrogel includes: subjecting the polyvinyl alcohol hydrogel to sequential mechanical stretching, salting-out fixation, freeze-drying, and annealing to construct an anisotropic structure, followed by immersion in a tannic acid solution for reinforcement. The hydrogel exhibits high crystallinity, excellent mechanical properties, and an extremely low equilibrium swelling rate, with a swelling rate of only 4.8% in phosphate buffered saline. The introduction of tannic acid endows the hydrogel with broad-spectrum antibacterial, antioxidant, and anti-inflammatory functions. The anisotropic structure, in conjunction with tannic acid, can significantly promote the directional migration of endothelial cells, angiogenesis, and the orderly regeneration of epithelium and smooth muscle. Animal experimental results show that this hydrogel can effectively reduce the recurrence rate of urethral stricture and achieve scarless functional repair.

The invention discloses cell-free fat extract loaded GelMA hydrogel and skin flap survival application, and particularly relates to the technical field of biomedical engineering. The hydrogel is prepared from GelMA, Ceffe, lithium phenyl (2, 4, 6-trimethylbenzoyl) phosphonate and a phosphate buffer salt solution. Wherein the Ceffe is rich in a plurality of angiogenesis promoting factors and is obtained through physical emulsification and centrifugal extraction; gelMA is prepared by modifying pig A-type gelatin through methacrylic anhydride, and has temperature-sensitive and photo-crosslinking characteristics. The injectable hydrogel with a porous network structure is formed by mixing Ceffe and a GelMA solution and curing through ultraviolet irradiation. The compound system realizes Ceffe biphasic slow release, remarkably promotes proliferation, migration and tube formation of vascular endothelial cells in vivo and in vitro, improves the survival rate of random skin flaps of mice, and effectively relieves oxidative stress injury caused by ischemia reperfusion by up-regulating HO-1 and down-regulating INOS.

The invention discloses a method for extracting hair papilla cells of back hair follicles of adult mice, and relates to the technical field of cell engineering. Comprising the following steps: removing redundant epidermis of an adult mouse by using an enzyme digestion method to obtain relatively pure dermis, carrying out secondary enzyme digestion, removing redundant dermis, only retaining a dermis layer suspension in which hair papilla is located, sequentially carrying out variable-speed and high-speed centrifugation for three times by using PBS (Phosphate Buffered Saline) resuspension and low-speed centrifugation for three times by using a complete culture medium according to different gravities of tissues, and carrying out high-speed centrifugation for three times by using the complete culture medium. Carrying out six times of co-gravity gradient centrifugation, observing that the hair follicle loses the original structure, the hair papilla tissue is dissociated from the root of the hair follicle and a sphere-like structure with a clear boundary is kept, carrying out resuspension culture by using a complete culture medium, and carrying out digestion and passage after the hair papilla cells migrate and grow from the tissue and are collected to 80-90%. The method is different from a traditional pure mechanical stripping method and an enzyme digestion method, so that the hair papilla gets rid of mechanical injury, a stereoscopic microscope is not needed, time and labor are wasted, the extracted hair papilla is high in adherence rate, the migration and growth speed of hair papilla cells is high, and the method solves the pain point that the back hair of the mouse cannot be stripped due to small size, and has a good application prospect. The problem that the hair papilla cells are difficult to grow due to insufficient cell dryness of the adult mice is also solved, the method can be used for extracting the back hair papilla cells of the adult mice for the first time, and the method is also suitable for extracting the antenna hair papilla cells of the adult mice.

The invention discloses a preparation method of nano hydrogel for reversing active oxygen and improving skin aging, and relates to the technical field of cosmetics, and the preparation method comprises the following steps: step 1, stirring and reacting dextran and sodium periodate in phosphate buffered saline water in a dark place at normal temperature to obtain oxidized dextran; 2, beta-cyclodextrin CD-NH2 is dissolved in PBS, and the two solutions are heated and stirred for a reaction; 3, ROS response groups and matrix metalloproteinase response polypeptides are added and stirred, and a mixture solution is obtained; 4, dissolving the drug astaxanthin in an organic solvent, adding the drug astaxanthin and tranexamic acid into the mixed solution for loading, and dialyzing at room temperature; and 5, sequentially extruding the solution on a PC film for multiple times by using a liposome extruder to obtain the nano hydrogel with ROS response performance. The problems that an existing formula is poor in stability, low in transdermal absorption rate and insufficient in synergistic effect are solved.

The invention discloses a preparation method and application of injectable bone repair hydrogel with an antioxidant function, and relates to the technical field of biomedical materials. The preparation method comprises the following steps: preparing Cu / Zn HA powder; the preparation method comprises the following steps: dissolving phenyl-2, 4, 6-trimethylbenzoyl lithium phosphinate in phosphate buffer saline water, and stirring to obtain an initiator solution; cu / Zn HA powder is added into the initiator solution, ultrasonic treatment is carried out after reaction, and a mixed solution is obtained; adding epigallocatechin gallate powder into the mixed solution, stirring and dissolving, then adding methacrylated gelatin, continuously stirring, injecting into a mold, and carrying out illumination crosslinking to obtain the injectable bone repair hydrogel. The injectable bone repair hydrogel disclosed by the invention has better proliferation promoting property, osteoinductivity and mechanical strength, is an ideal material suitable for subchondral bone repair, and has a good clinical application prospect.

The application provides a composite preservative for fecal microorganisms and metabolites thereof and a same-tube multi-omics joint detection integrated technology. The composite preservative contains main components of cetyltrimethylammonium bromide (CTAB), ammonium chloride (NH4Cl) and phosphate buffered saline (PBS), can keep the stability of microorganisms and metabolites thereof in a fecal sample during storage and transportation at room temperature, and guarantees the extraction efficiency of deoxyribonucleic acid (DNA) of the microorganisms in the fecal sample. The application also eliminates the influence of the preservative components on metabolite detection by adding a treatment reagent, significantly improves the response and ionization efficiency of the fecal sample metabolites in mass spectrometry, and thus improves the sensitivity and accuracy of the detection results. The application not only solves the timeliness problem of fecal sampling, but also provides a technical basis and idea for the feasibility study of the same-tube multi-omics of the fecal sample, and has a broad research and clinical application prospect.

The disclosure provides marine polysaccharide vector-based anthocyanin nanoparticles, using marine polysaccharides, food-borne fluorescent nanoparticles and sodium hyaluronate as a composite vector to be combined with anthocyanin, and is prepared by a method: S1, dissolving sodium alginate in phosphate buffered saline, and successively adding EDC, HOBt and the food-borne fluorescent nanoparticles to obtain a fluorescence-alginate complex; S2, adding the fluorescence-alginate complex into a mixture of DMF and DCM, and then adding DIC and DMAP; S3, adding the anthocyanin and hyaluronic acid, and then removing a solvent; and S4, removing unreacted small molecules, and carrying out freeze drying.