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Angiogenesis-facilitating temperature-sensitive hydrogel powder and temperature-sensitive hydrogel prepared from same

A hydrogel powder, temperature-sensitive technology, applied in medical science, absorbent pads, bandages, etc., to achieve the effects of convenient mass production and market use, low environmental requirements, and easy operation

Active Publication Date: 2014-12-24
杭州倍荣生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no research on decellularization of human placenta after freezing for the preparation of hydrogel at home and abroad

Method used

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  • Angiogenesis-facilitating temperature-sensitive hydrogel powder and temperature-sensitive hydrogel prepared from same
  • Angiogenesis-facilitating temperature-sensitive hydrogel powder and temperature-sensitive hydrogel prepared from same
  • Angiogenesis-facilitating temperature-sensitive hydrogel powder and temperature-sensitive hydrogel prepared from same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Embodiment 1 Preparation of thermosensitive hydrogel powder of the present invention

[0097] 1. Decellularization of human placenta

[0098] (1) Take the human placenta (fresh or frozen) that has been delivered normally, has undergone serological testing, and removed the amnion, pre-frozen at -40°C, cut into thin slices with a thickness of about 1mm (about 2cm×2cm), and washed with a large amount of normal saline until The solution has no blood red;

[0099] (2) soak in 0.5% SDS physiological saline solution for 8 hours with shaking (change the solution 4 times in the middle), and rinse with deionized water;

[0100] (3) After 30 minutes of treatment with 0.1% peracetic acid and 20% ethanol mixed solution, rinse with deionized water;

[0101] (4) Freeze-drying (the freeze-drying temperature is -80° C., the vacuum degree is 0.12 mbar, and the time is 24 hours) to obtain decellularized human placenta tissue, namely DPM.

[0102] Keep the materials obtained in the prev...

Embodiment 2

[0107] Embodiment 2 Preparation of thermosensitive hydrogel powder of the present invention

[0108] 1. Decellularization of human placenta

[0109] (1) Take the human placenta (fresh or frozen) that has been delivered normally, has undergone serological testing, and removed the amnion, pre-frozen at -40°C, cut into thin slices with a thickness of about 1mm (about 2cm×2cm), and washed with a large amount of normal saline until The solution has no blood red;

[0110] (2) Soak in 1% SDS physiological saline solution for 8 hours with shaking (change the liquid 4 times in the middle, the shaking frequency is 100rpm), rinse with deionized water;

[0111] (3) After 30 minutes of treatment with 0.1% peracetic acid and 20% ethanol mixed solution, rinse with deionized water;

[0112] (4) freeze-drying (the freeze-drying temperature is -50° C., the vacuum degree is 0.1 mbar, and the time is 48 hours), and the decellularized human placenta tissue, ie, DPM, is obtained.

[0113] Keep t...

Embodiment 3

[0118] Embodiment 3 Preparation of thermosensitive hydrogel powder of the present invention

[0119] 1. Decellularization of human placenta

[0120] (1) Take the human placenta (fresh or frozen) that has been delivered normally, has undergone serological testing, and removed the amnion, pre-frozen at -40°C, cut into thin slices with a thickness of about 1mm (about 2cm×2cm), and washed with a large amount of normal saline until The solution has no blood red;

[0121] (2) Soak in 0.5% SDS physiological saline solution for 15 hours with shaking (4 times in the middle, the frequency of shaking is 100 rpm), rinse with deionized water;

[0122] (3) After 30 minutes of treatment with 0.1% peracetic acid and 20% ethanol mixed solution, rinse with deionized water;

[0123] (4) freeze-drying (the freeze-drying temperature is -60° C., the vacuum degree is 0.2 mbar, and the time is 36 hours), and the decellularized human placenta tissue, ie, DPM, is obtained.

[0124] Keep the material...

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Abstract

The invention discloses a preparation method of angiogenesis-facilitating temperature-sensitive hydrogel. The preparation method comprises the following steps: under an ice-bath condition, adding the temperature-sensitive hydrogel powder provided by the invention and used as a raw material into a precooled PBS (phosphate buffer saline) solution, distilled water or DMEM (dulbecco's modified eagle medium), wherein the ratio of the temperature-sensitive hydrogel powder to the precooled PBS solution, distilled water or DMEM is 30mg:1ml, uniformly stirring, and placing the mixture in an environment with the temperature of 37 DEG C, so as to obtain the angiogenesis-facilitating temperature-sensitive hydrogel. The invention further discloses novel temperature-sensitive hydrogel powder and a preparation method thereof. The temperature-sensitive hydrogel powder can be stored for a long time at room temperature, the sterilization method for the temperature-sensitive hydrogel powder is simple, and the temperature-sensitive hydrogel powder can be conveniently produced in a large-scale manner and put into a market; the temperature-sensitive hydrogel prepared from the temperature-sensitive hydrogel powder has good temperature sensitiveness and biocompatibility, can be degraded in a body, has an angiogenesis-facilitating effect, and has a good application prospect in regenerative medicine and clinical treatment.

Description

technical field [0001] The invention relates to a temperature-sensitive hydrogel powder and a temperature-sensitive hydrogel prepared therefrom, belonging to the field of biological materials. Background technique [0002] Hydrogel is a gel with water as the dispersion medium. It has a water content close to normal tissue and good biocompatibility. Its physical and chemical properties are controllable and it has a porous structure. Its three-dimensional porous structure is very similar to the extracellular matrix. , is conducive to cell growth, proliferation and differentiation, and is also conducive to the maintenance of the activity of biologically active factors. It can be minimally invasively implanted and has broad application prospects in tissue engineering. [0003] Thermosensitive hydrogel can change from liquid state to gel state within the body temperature range, without other catalysts and severe reaction conditions, can repair various irregular-shaped tissue defe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/40A61L15/42A61L15/64
Inventor 罗静聪晁宁宁解慧琪
Owner 杭州倍荣生物科技有限公司
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